Commercial Scale Tissue Culture For Horticulture And Plantation Crops

This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops. This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercial plant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors. This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.

In 1980, a conference on tissue culture of fruit crops was held at Beltsville to summarize the current status of this technology and to stimulate interest in it among research scientists, students, and commercial producers in the U. S. Interest in that conference and the proceedings from it far exceeded the expectations of the organizing committee. Since that time, micropropagation of fruit crops in the U. S. has increased significantly, but still lags far behind applications to production of ornamental plants. Within the past two years, a number of new laboratories have been established and some of the existing laboratories have expanded to a size far larger than any previously anticipated. Creation of new laboratories capable of producing more than 400,000 plants per week will test the ingenuity of laboratory managers and the skills of marketing departments. In recent years, numerous symposia have been held on various aspects of biotechnology and genetic engineering. Although micro propagation is the key to providing large numbers of genetically engineered plants, it is a topic that has been relegated to a minor position, or ignored completely, at such meetings. Accordingly, the time seemed propitious for a conference devoted solely to all aspects of micropropagation as applicable to horticultural crops.

This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.

This symposium is the third in a series featuring the propaga tion of higher plants through tissue culture. The first of these symposia, entitled "A Bridge Between Research and Application," was held at the University in 1978 and was published by the Technical Information Center, Department of Energy. The second symposium, on "Emerging Technologies and Strategies," was held in 1980 and pub lished as a special issue of Environmental and Experimental Botany. One of the aims of these symposia was to examine the current state of-the-art in tissue culture technology and to relate this state of technology to practical, applied, and commercial interests. Thus, the third of this series on development and variation focused on embryogenesis in culture: how to recognize it, factors which affect embryogenesis, use of embryogenic systems, etc.; and variability from culture. A special session on woody species again emphasized somatic embryogenesis as a means of rapid propagation. This volume emphasizes tissue culture of forest trees. All of these areas, we feel, are breakthrough areas in which significant progress is expected in the next few years.

Handbook containing basic information on tissue-culture techniques and their application in the nursery industry. Geared towards people interested in establishing a commercial tissue- culture laboratory and/or handling tissue-cultured plants in a nursery.

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

This reference is a timely compilation of studies of genome size and genetic stability of regenerated plants. It presents 13 book chapters that cover recent advancements in CRISPR/Cas-based genome editing, the use of molecular markers to analyze somaclonal variation in tissue culture, and genetic stability assessment in various plant species, including medicinally valuable plants like Valeriana and Coffea. The book also highlights the role of flow cytometry in investigating polyploidy and provides valuable insights into genetic fidelity assessment of micropropagated woody plants and orchids.The contributors have shed light on the intra-specific and inter-specific genome and chromosome number variation with reference to gene duplication and DNA sequence loss. Molecular techniques for detecting ploidy levels and genetic homogeneity in regenerated plantlets are also discussed. Additional highlights of the book include brief guidelines for experimental protocols for flow cytometry and molecular markers, coverage of a wide range of plants, and supporting references. This is an excellent reference for biologists, geneticists, and plant scientists exploring genetic homogeneity and genome size variation in diverse plant groups.