Comprehensive Laboratory Manual Of Life Sciences

The present book ‘Comprehensive Laboratory Manual of Life Science’, deals with practical trends in modern biological sciences. It furnishes protocols on recent advances in biotechnological methods and aims to cover three most important aspects of this interdisciplinary stream; such as Microbiology, Biochemistry and Molecular biology. The book contains four sections: 1. Introduction: emphasizes on good laboratory practices and etiquettes for beginners; the do’s and don’ts of working in a laboratory, concepts and terminology, etc. 2. Instruments: Principle and Precautions: explores commonly used equipments employed in different experiments. 3. Experiments: is further divided into three parts: Microbiology with more than 70 experiments, Biochemistry with 62 and Molecular Biology having around 32 detailed protocols, accorded to make the readers proficient in the paramount disciplines of Bio Sciences and Biotechnology. 4. Appendix: at the end, a rather comprehensive section that concludes the book. This book is designed to meet the practical requirements of undergraduate and post graduate students of Life Science, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering by providing worked out solution to the most commonly practiced experiments prescribed by majority of Indian Universities. The latest technological developments in the book will be appealing to the researchers and scientists

Basic Life Science Methods: A Laboratory Manual for Students and Researchers presents forty of the most executed life science assays. The authors use a consistent structure to cover the preparation, execution and analysis of data from each method. Assays include estimation of cholesterol fractions, C-Reactive Protein, Genomic DNA isolation, Agarose Gel Electrophoresis, RT-PCR, DNA solution preparation, how to design primers, and enzyme-linked immunosorbent assay (ELISA). This book provides a complete reference containing step-by-step instructions on how to run life science assays. Laboratory staff can also benefit of the book as a training resource. Provides a practical resource on designing, executing and analyzing experiments and analytical procedures Includes detailed and standardized coverage of basic research methods in the area Presents step-by-step instructions on how to execute a large selection of life sciences experiments

This book aims to provide basic practical guidelines for microbiology and biotechnology students. All experiments have been carefully written in a clear and concise form. Major topics covered include basic microbiology practical's including antibiotic sensitivity test, Gram’s staining, Methylene Blue Reductase (MBRT) Test, Streak plate method and Food technology methods such as determination of detergent in milk, to separate the plant pigments in a food sample. This book also provides basic bioinformatics practical guidelines that can be used in dairy and food microbiology. For undergraduate (B. Sc. & B. Tech) and graduate (M. Sc.) students in various branches of biology, the book presents up-to-date fundamental information about the significant aspects of dairy microbiology as well as food microbiology. Personnel in the food industry who have little to no background in microbiology or need a refresher course in fundamental microbiological concepts and laboratory procedures will also find this book useful.

This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results

cell and molecular biology laboratory manual 2009

This laboratory manual, suitable for biology majors or non-majors, provides a selection of lucid, comprehensive experiments that include excellent detail, illustration, and pedagogy.