Computer Operation For Microscope Photometry

Suitable for both microscopists seeking computer skills and PC enthusiasts interested in light microscopy, this interdisciplinary text explores the capabilities of the computer-assisted light microscope. Written in clear, simple language, the book explains how computer technology expands the usefulness of the light microscope in spectrophotometry, fluorometry, polarimetry, spatial scanning, and related fields. Beginning with the basic features of light microscopy and personal computer interfacing, the text explains how to make photometric measurements and covers spectrophotometry, stepper motors, and server motors. Polarized light and video image analysis complete this introduction to the field. While software examples are provided to illustrate specific techniques, most operations are described as generalized algorithms that can be adapted to any appropriate high-level language, and used with almost any configuration of the microscope. The book suggests new experiments to inspire further study. Promising new areas of interest, such as the use of fluorescence and polarization, are also included. Computers have radically changed the field of light microscopy in recent decades. Computer Operations for Microscope Photometry helps you master the new techniques.

Over the last dozen years there has been a great development in the study of matter on the micro-scale by means of the light microscope, which has coincided with the rapid growth of auto mation and the use of computers. The manufacturers have responded to this challenge, and there is now available a large choice of modules for use with the microscope, both on the biological and non-biological side. This book is on microscope photometry. Photometry on the macro-scale can be applied to features of not less than about one millimetre in size. Below this lies the realm of microscope photometry which will be discussed both in theory and in practice. As a general rule no discussion of technical design of equipment is included, as such information is available in the handbooks of the particular manufacturers of equipment. It attempts to cover all the uses of the optical microscope photometer with its auxiliary equipment, and the first chapter describes the topics covered. In using the word' light' we apply its meaning to extend from UV to near IR. No attempt has been made to list all the works consulted. For each subject treated in this book a list is given of books and papers considered to be of key importance; in these will be found more detailed bibliographies.

Over the last dozen years there has been a great development in the study of matter on the micro-scale by means of the light microscope, which has coincided with the rapid growth of auto mation and the use of computers. The manufacturers have responded to this challenge, and there is now available a large choice of modules for use with the microscope, both on the biological and non-biological side. This book is on microscope photometry. Photometry on the macro-scale can be applied to features of not less than about one millimetre in size. Below this lies the realm of microscope photometry which will be discussed both in theory and in practice. As a general rule no discussion of technical design of equipment is included, as such information is available in the handbooks of the particular manufacturers of equipment. It attempts to cover all the uses of the optical microscope photometer with its auxiliary equipment, and the first chapter describes the topics covered. In using the word' light' we apply its meaning to extend from UV to near IR. No attempt has been made to list all the works consulted. For each subject treated in this book a list is given of books and papers considered to be of key importance; in these will be found more detailed bibliographies.

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