Dental Stem Cells Regenerative Potential

This book focuses on the basic aspects of dental stem cells (DSCs) as well as their clinical applications in tissue engineering and regenerative medicine. It opens with a discussion of classification, protocols, and properties of DSCs and proceeds to explore DSCs within the contexts of cryopreservation; epigenetics; pulp, periodontal, tooth, bone, and corneal stroma regeneration; neuronal properties, mesenchymal stem cells and biomaterials; and as sources of hepatocytes for liver disease treatment. The fifteen expertly authored chapters comprehensively examine possible applications of DSCs and provide invaluable insights into mechanisms of growth and differentiation. Dental Stem Cells: Regenerative Potential draws from a wealth of international perspectives and is an essential addition to the developing literature on dental stem cells. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is indispensable for biomedical researchers interested in bioengineering, dentistry, tissue engineering, regenerative medicine, cell biology and oncology.

This book provides evidence-based information in the field of regenerative dentistry discussing the most recent advances, current clinical applications, limitations and future directions. The coverage encompasses the regeneration of alveolar bone, the dentine-pulp complex, enamel, the periodontium and other tissues associated with the oral cavity. A full description is provided of regenerative approaches in dentistry including regenerative endodontics and tooth repair, regenerative periodontics, regenerative assisted orthodontics, regenerative approaches in oral medicine, and dental tissue derived stem cells and their potential applications. The book is written by an international team of leading experts. It will be beneficial for students, practitioners and researchers in the fields of endodontics, periodontics and implantology.

This book explores Dental Stem Cell (DSC) biology, from a review of basic concepts for cell culture, to isolation, self-renewal, multipotency and differentiation, regulation by molecular medicine, and prospective research areas for regenerative medicine. The first seven chapters delve into basic DSC properties, vital signaling pathways involved in differentiation, pluripotency, iPS cell development from DSCs, and genetic engineering approaches of DSCs in accordance with the current literature. A comprehensive review of possible clinical applications and in vitro/in vivo studies follows, illustrating the future of DSC research for in the tissue engineering field. The text also discusses the political, ethical, social, and legal ramifications of the use of dental stem cells. Expertly authored and drawing from a multitude of international perspectives, Dental Stem Cells is an invaluable addition to Springer’s Stem Cell Biology and Regenerative Medicine series. It is essential reading for advanced graduate students, basic researchers, and clinical investigators in the fields of stem cell therapy, biological sciences of dentistry, and regenerative medicine.

Project Report from the year 2018 in the subject Medicine - Medical Frontiers and Special Areas, grade: 1, Egerton University, language: English, abstract: This paper will provide a comprehensive review on the origin and types of MSCs in the dental tissue and the oral cavity. It will also discuss their therapeutic mechanisms that make them useful in dentistry and dental treatments. Over the past few decades, stem cell research has gained extensive scientific inquiry. This aspect is attributable to the significance of stem cells in tissue engineering. It is apparent that tissue regeneration has emerged as a reliable medical approach for the treatment of tissue disorders and injuries. Initially, embryonic stem cells were preferred as candidates for regenerative medicine because these cells can be induced to replicate in a pluripotent state. However, stem cell research involving embryonic stem cells has attracted immense controversy. It is also associated with legal and ethical issues, thus limiting the use of embryonic stem cells in regenerative medicine. Fortunately, the discovery of mesenchymal stem cells (MSCs), also referred to as adult stem cells, has restored promise for the development of stem cell therapies. Unlike embryonic stem cells, MSCs are free from legal and ethical concerns. MSCs are usually pluripotent progenitor cells that are generated in an array of tissues in both foetal and adult life. It is reported that these progenitor cells differentiate into cell types of the tissues that generate them, although studies indicate that they can differentiate cell types of other tissues. Currently, MSCs are used for regenerative therapies for a number of tissue disorders and injuries including bone regeneration. For instance, MSCs generated by the dental pulps and the oral cavity tissues have been found to possess the potential for dental tissue regeneration. These cells have also been found to useful in non-dental tissue repair.

This volume covers all aspects of embryonic stem cell differentiation, including mouse embryonic stem cells, mouse embryonic germ cells, monkey and human embryonic stem cells, and gene discovery. * Early commitment steps and generation of chimeric mice * Differentiation to mesoderm derivatives * Gene discovery by manipulation of mouse embryonic stem cells

Dental caries, periodontitis, tooth loss, and bone resorption are considered prevalent health problems that have direct affect on the quality of life. While, advances in stem cell biology and biotechnology have sparked hope for devastating maladies, such as diabetes, cardiovascular diseases, etc., it also provides a strategy of regenerative therapy for dental tissues. From the prospective of tissue engineering, it is of utmost importance to understand and emulate the complex cell interactions that make up a tissue or organ. Unlike other tissues in the body, dental tissues are unique in their development, function, and even in their maintenance throughout life. The harmonized stimulations of biology and mechanical regulators to promote cellular activities have matured our understanding of the value of regenerative therapy of dental tissue versus the reparative treatment. In this book, we review the current knowledge available to regenerate alveolar bone, periodontal structure, and pulp/dentin complex. The book provides researchers with detailed information about development and functional characteristics of the dental unit with detailed protocols covering a comprehensive range of various approaches to engineer dental tissues: to use isolated cells or cell substitutes as cellular replacement, to use acellular biomaterials capable of inducing tissue regeneration, and/or to use a combination of cells, biomaterial and growth factors. We are well aware, with the concept changes in the field toward in-vitro biomimetics of in-vivo tissue development. The theoretical frame work integrating these concepts of developmental biology and developmental engineering is yet to be emphasized and implemented. Until this happens, we consider this book of regenerative dentistry as a call for scientists to achieve, researchers to innovate, practitioners to apply, and students to learn the art and science of regenerative therapy in dentistry. Table of Contents: Introduction to Regenerative Dentistry / Tissue Engineering Alveolar Bone / Tissue Engineering of the Periodontal Tissues / Dynamics for Pulp-Dentin Tissue Engineering in Operative Dentistry

This volume looks at the state-of-the-science in stem cells, discusses the current challenges, and examines the new directions the field is taking. Dr. Turksen, editor-in-chief of the journal "Stem Cell Reviews and Reports," has assembled a volume of internationally-known scientists who cover topics that are both clinically and research-oriented. The contents range from sources of stem cells through their physiological role in health and disease, therapeutic applications in regenerative medicine, and ethics and society. An initial overview and a final summary bookend the contents into a cohesive and invaluable volume.