Determination Of The Size And Shape Of Protein Molecules

Analytical Methods of Protein Chemistry, Volume 3: Determination of the Size and Shape of Protein Molecules provides information pertinent to the analysis and isolation of protein. This book deals with the measurement of the macromolecular properties of proteins. Organized into seven chapters, this volume begins with an overview of the theory and practice of the electron microscope to allow an understanding of the type of object that may be examined. This text then describes the methods of making protein molecules conform to such an ideal, which are the techniques of specimen preparation. Other chapters consider the determinations of osmotic pressures of proteins. This book discusses as well the experimental basis for the theory of the diffusion process in liquids. The final chapter deals with the technical problem characteristics of light-scattering. This book is a valuable resource for electron microscopists, protein chemists, biologists, physicist, physico-chemists, scientists, and research workers.

Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.

Protein Structure deals with the chemistry and physics of biologically important molecules—the proteins—particularly the determination of the structure of various proteins, their thermodynamics, their kinetics, and the mechanisms of different reactions of individual proteins. The book approaches the study of protein structure in two ways: firstly, by determining the general features of protein structure, the overall size, and shape of the molecule; and secondly, by investigating the molecule internally along with the various aspects of the internal configuration of protein molecules. It describes in detail experimental methods for determining protein structure in solution, such as the hydrodynamic method, the thermodynamic optical method, and the electrochemical method. The book then explains the results of experiments carried out on insulin, lysozyme, and ribonuclease. The text notes that the experiments, carried out on native and denatured proteins as well as on derivatives prepared by chemical modification (e.g., by methylation, iodination, acetylation, etc.), can lead to greater understanding of secondary and tertiary structures of proteins of known sequence. The book is suitable for biochemists, micro-biologists, cellular researchers, or investigators involved in protein structure and other biological sciences related to muscle physiologists, geneticists, enzymologists, or immunologists.

In the last fifteen years there has been a revolution in the techniques available for the analysis and isolation of proteins. Every time a new technique has been introduced, numerous papers have appeared describing modifications to it and the research worker who wishes to employ these methods is faced with a very serious problem in deciding which particular variant to use. These volumes are intended to provide the fullest practical detail so that any scientist can follow the procedure by using this book alone and without having recourse to the original literature. The techniques which are described in full are ones in which all the authors have had first-hand experience, and the descriptions contain those small but important points which save so much time. In the first volume, separation and isolation procedures are discussed; the second concerns its analysis and reactivity, and the third volume with the measurement of the macromolecular properties of proteins.

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid