Dna And Rna Isolation Techniques For Non Experts

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

In this book, the authors present current research in the study of DNA binding and extraction methods, applications, and limitations. Topics discussed in this compilation include DNA binding to cationic polymers and its implications on gene therapy; metal based anticancer drug-DNA binding; non-covalent DNA-AuNP's interactions; prospects of nanoparticle DNA binding; extraction and purification of DNA from USAB reactor sludge samples; isolation of genomic DNA from microbes and microbial communities; dengue virus RNA extraction challenges and isolating bacterial and viral nucleic acids from clinical samples for diagnostic testing.

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

Medical Microbiology deals with the study of microorganisms that colonize or infect humans. It studies the pathogenesis and epidemiology of these microorganisms with practical applications in diagnosis, treatment and prevention of diseases (infectious diseases, infection-related cancer, dysbiosis related diseases, etc.,). In the past two decades, its expansion is essentially due to the great advances in related fields such as immunology, genetics, bioinformatics, science, technology, engineering and mathematics. New scientific knowledge and understanding make possible new applications. Nevertheless, it becomes a challenge due to lack of expertise to analyze fast-growing data and to translate them in clinical practice and medical education. The pace and sophistication of advances in all scientific disciplines applied to medical microbiology call for a comprehensive and integrative understanding of the overall knowledge development in the different related fields (immunology, genetics, bioinformatics, science, technology, engineering and mathematics, etc.,). In light of this, the main goal of this topical collection is to bridge the gap between all these fields. Each article in this compilation is a stand‐alone text based on an integrative review, which highlights advances in molecular diagnosis and epidemiology of human pathogens. It also helps researchers and practitioners to access and scrutinize the fast expanding knowledge. This research topic in medical microbiology provides an updated core of basic knowledge critical to clinical practice in medicine. It is designed for physicians, researchers, educators, residents, Lab technicians, undergraduate and graduate students. Each article deals with an important human pathogen. It uses a multidisciplinary approach integrating new advances, especially in genetics, bioinformatics, science, technology, engineering and mathematics. It provides an update of the current state-of-the-art scientific knowledge applied to molecular diagnosis and epidemiology of human pathogens. Rapid detection, accurate identification and typing of causative pathogens are necessary to optimize treatment, to trace pathogens and to study microbial population dynamics.