Dna Arrays
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DNA Arrays
Author | : Jang B. Rampal |
Publsiher | : Springer Science & Business Media |
Total Pages | : 268 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 9781592592340 |
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Microarray technology provides a highly sensitive and precise te- nique for obtaining information from biological samples, with the added advantage that it can handle a large number of samples simultaneously that may be analyzed rapidly. Researchers are applying microarray technology to understand gene expression, mutation analysis, and the sequencing of genes. Although this technology has been experimental, and thus has been through feasibility studies, it has just recently entered into widespread use for advanced research. The purpose of DNA Arrays: Methods and Protocols is to provide instruction in designing and constructing DNA arrays, as well as hybridizing them with biological samples for analysis. An additional purpose is to p- vide the reader with a broad description of DNA-based array technology and its potential applications. This volume also covers the history of DNA arrays—from their conception to their ready off-the-shelf availability—for readers who are new to array technology as well as those who are well versed in this field. Stepwise, detailed experimental procedures are described for constructing DNA arrays, including the choice of solid support, attachment methods, and the general conditions for hybridization. With microarray technology, ordered arrays of oligonucleotides or other DNA sequences are attached or printed to the solid support using au- mated methods for array synthesis. Probe sequences are selected in such a way that they have the appropriate sequence length, site of mutation, and T .
DNA Arrays
Author | : Elena V. Grigorenko |
Publsiher | : CRC Press |
Total Pages | : 183 |
Release | : 2001-12-21 |
Genre | : Science |
ISBN | : 9781420038859 |
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A quantum leap in technology took place a few years ago with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. DNA Arrays: Technologies and Experimental Strategies offers a view of different aspects of this rapidly developing technology, in
DNA Microarrays and Gene Expression
Author | : Pierre Baldi,G. Wesley Hatfield |
Publsiher | : Cambridge University Press |
Total Pages | : 238 |
Release | : 2011-04-28 |
Genre | : Science |
ISBN | : 1139437607 |
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Massive data acquisition technologies, such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. Using the mRNA of a given cell, at a given time, under a given set of conditions, DNA microarrays can provide a snapshot of the level of expression of all the genes in the cell. Such snapshots can be used to study fundamental biological phenomena such as development or evolution, to determine the function of new genes, to infer the role individual genes or groups of genes may play in diseases, and to monitor the effect of drugs and other compounds on gene expression. Originally published in 2002, this inter-disciplinary introduction to DNA arrays will be of value to anyone with an a interest in this powerful technology.
DNA Microarrays Gene Expression Applications
Author | : B.R. Jordan |
Publsiher | : Springer Science & Business Media |
Total Pages | : 139 |
Release | : 2013-12-01 |
Genre | : Medical |
ISBN | : 9783642565175 |
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This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book.
Design and Analysis of DNA Microarray Investigations
Author | : Richard M. Simon,Edward L. Korn,Lisa M. McShane,Michael D. Radmacher,George W. Wright,Yingdong Zhao |
Publsiher | : Springer Science & Business Media |
Total Pages | : 205 |
Release | : 2006-05-09 |
Genre | : Medical |
ISBN | : 9780387218663 |
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The analysis of gene expression profile data from DNA micorarray studies are discussed in this book. It provides a review of available methods and presents it in a manner that is intelligible to biologists. It offers an understanding of the design and analysis of experiments utilizing microarrays to benefit scientists. It includes an Appendix tutorial on the use of BRB-ArrayTools and step by step analyses of several major datasets using this software which is available from the National Cancer Institute.
DNA Microarrays
Author | : Mark Schena |
Publsiher | : Practical Approach Series |
Total Pages | : 236 |
Release | : 1999 |
Genre | : Medical |
ISBN | : 0199637768 |
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DNA Microarrays: A Practical Approach is the first comprehensive overview of an exciting and powerful new technology. DNA microarrays, or biochips, are small glass chips embedded with ordered rows of DNA, providing a massive parallel platform for data gathering and representing a fundamental technical advance in biomedical research. Such biochips gather data at an unprecedented rate by enabling the use of advanced fabrication, detection, and data mining technologies. Written and edited by experts in the field, this book provides fascinating insight into this remarkable advancement. It opens with an introduction to the technology of DNA microarrays, emphasizing the methodological fundamentals of biochips, and continues with descriptions of the use of confocal scanning in microarray detection and techniques for the efficient cloning and screening of differentially expressed genes. The chapters address many topics, among them assay optimization, antisense scanning arrays, the manufacture of molecular arrays, and gene expression analysis. Also addressed are the uses of expression data in bioinformatics, of active microelectronic arrays for DNA hybridization analysis, and of microarray technology in pharmacogenomics. DNA Microarrays: A Practical Approach is ideal for researchers investigating patterns of gene expression (and the relationship with disease), and it is essential for any researcher interested in the use of biochips.
DNA Arrays in Neurobiology
Author | : Anonim |
Publsiher | : Elsevier |
Total Pages | : 218 |
Release | : 2004-12-10 |
Genre | : Medical |
ISBN | : 9780080495514 |
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DNA array technology is a technique for studying gene expression by comparing samples of different genes. The result is an enormous amount of data that must be carefully analyzed in order for it to be useful and meaningful. This book examines both data analysis and techniques for ensuring optimal experimental conditions. The array approach has applications in a number of model systems, including development, learning and drug abuse. In addition, the technique has applications in a number of neurological disorders such as Alzheimer's disease, schizophrenia, multiple sclerosis, and neurological cancers.
DNA Microarrays
Author | : Ulrike Nuber |
Publsiher | : Taylor & Francis |
Total Pages | : 320 |
Release | : 2007-01-24 |
Genre | : Science |
ISBN | : 9781134243990 |
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"DNA Microarrays" introduces the reader to the different types of DNA microarrays and their various applications. Furthermore, several data analysis methods are described. It is written for scientists who are entering the field of DNA microarrays, as well as for those already familiar with the technology, but interested in new applications and methods.