Electron Microscopy Of Diatom Cells

DIATOM MICROSCOPY The main goal of the book is to demonstrate the wide variety of microscopy methods being used to investigate natural and altered diatom structures. This book on Diatom Microscopy gives an introduction to the wide panoply of microscopy methods being used to investigate diatom structure and biology, marking considerable advances in recent technology including optical, fluorescence, confocal and electron microscopy, surface-enhanced Raman spectroscopy (SERS), atomic force microscopy (AFM) and spectroscopy as applied to diatoms. Each chapter includes a tutorial on a microscopy technique and reviews its applications in diatom nanotechnology and diatom research. The number of diatomists, diatom research, and their publications are increasing rapidly. Although many books have dealt with various aspects of diatom biotechnology, nanotechnology, and morphology, to our knowledge, no volume exists that summarizes advanced microscopic approaches to diatoms. Audience The intended audience is academic and industry researchers as well as graduate students working on diatoms and diatom nanotechnology, including biosensors, biomedical engineering, solar panels, batteries, drug delivery, insect control, and biofuels.

The book "The Transmission Electron Microscope" contains a collection of research articles submitted by engineers and scientists to present an overview of different aspects of TEM from the basic mechanisms and diagnosis to the latest advancements in the field. The book presents descriptions of electron microscopy, models for improved sample sizing and handling, new methods of image projection, and experimental methodologies for nanomaterials studies. The selection of chapters focuses on transmission electron microscopy used in material characterization, with special emphasis on both the theoretical and experimental aspect of modern electron microscopy techniques. I believe that a broad range of readers, such as students, scientists and engineers will benefit from this book.

This volume of Nova Hedwigia Beihefte includes papers covering a broad range of topics in diatom and chrysophyte research - including historical analyses of taxa names and name changes - through descriptions of novel taxa; observations of fine details of valve and cell morphology and ultrastructure, in many cases in an ecological and/or biostratigraphic context; to attempts at clarifying some hard-to-resolve questions on the evolutionary history of diatoms. Although there is no single common scientific thread connecting all contributions, they are all influenced, in some way, by the work of R. M. Crawford. Some highlight the still substantial extent of undiscovered terrain in the realm of diatom (and microalgal) ultrastructure, regarding variations on the fine construction of both frustules and organic cell components, an area of research in which R. M. Crawford laid important groundwork. Other papers add to our understandingof individual taxonomic groups in which R. M. Crawford raised a heightened interest, such as the genus Corethron which features the most elaborate movements of cell wall parts during cell division in diatoms, or the diverse genera of melosiroid diatoms where the work of R. M. Crawford was instrumental in recognizing the extent of (functional-)morphological and ecological variability. Combining diverse perspectives and methods, this volume will be of interest to researchers of diatoms, chrysophytes and other microalgal groups. The volume is dedicated to Richard M. Crawford, who spent a lifetime researching dinoflagellates and diatoms, particularly their morphology and taxonomy, utilizing various microscopical techniques. He is perhaps best known for his pioneering works on the dinoflagellate genera Oxyrrhis and Woloszynskia, and the centric diatoms, Aulacoseira, Melosira, Ellerbeckia, Paralia, and Corethron. He was one of the contributors to the famous blue book (The Diatoms: Biology & Morphology of the Genera) and was the curator to the Hustedt Diatom Collection in Bremerhaven.

Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.

In the continuing quest to explore structure and to relate struc tural organization to functional significance, the scientist has developed a vast array of microscopes. The scanning electron microscope (SEM) represents a recent and important advance in the development of useful tools for investigating the structural organization of matter. Recent progress in both technology and methodology has resulted in numerous biological publications in which the SEM has been utilized exclusively or in connection with other types of microscopes to reveal surface as well as intracellular details in plant and animal tissues and organs. Because of the resolution and depth of focus presented in the SEM photograph when compared, for example, with that in the light microscope photographs, images recorded with the SEM have widely circulated in newspapers, periodicals and scientific journals in recent times. Considering the utility and present status of scanning electron microscopy, it seemed to us to be a particularly appropriate time to assemble a text-atlas dealing with biological applications of scanning electron microscopy so that such information might be presented to the student and to others not yet familiar with its capabilities in teaching and research. The major goal of this book, therefore, has been to assemble material that would be useful to those students beginning their study of botany or zoo logy, as well as to beginning medical students and students in advanced biology courses.