Embryogenesis In Plants

This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.

In vitro Embryogenesis in Plants is the first book devoted exclusively to this topic. As the ultimate demonstration of totipotency in plants, somatic and haploid embryogenesis is of vital importance to all those working on or interested in basic and applied aspects of plantlet information and regeneration. The text includes comprehensive reviews written by experts, on all facts of in vitro and in vivo embryogenesis. Some chapters deal with the morphogenic, structural and developmental, physiological and biochemical, and molecular biological aspects of the subject. Chapters are also devoted to haploid embryogenesis, asexual embryogenesis in nature, zygotic embryogenesis, and zygotic embryo culture. Detailed tables summarizing successful somatic embryogenesis in all vascular plants are also included. This book, therefore, brings together previously scattered information to provide an indispensable reference book for both active researchers, graduate students and anyone interested in this aspect of tissue culture technology and plant development.

Vegetative Morphologie, Morphologie.

Somatic embryogenesis, the initiation of embryos from previously differentiated somatic cells, is a unique process in plants. This volume expands our view of a subject that is important for plant biotechnology, genetics, cell biology, development, and agricultural applications. All chapters present the latest research progress, including functional genomic, genetic, and proteomic approaches. A special focus is placed on the effects of stress, environment, and plant growth regulators on embryogenesis. The role of genes such as Leafy Cotyledons and Baby Boom in defining and maintaining cell competence is discussed.

Step-by-step reproducible laboratory procedures written by the top experts in the field offering their time-tested hints, tricks, and tips. Presents detailed outlines of embryonic systems models along with cutting-edge cellular, genetic, and molecular mechanism. Provides the most comprehensive collection of protocols on plan embryo development.

The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com mercial companies. Generally, tissue culture of woody plants has been recal citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalurn album), Citrus, Mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propa gation for large-scale production of plants or "emblings" or "somatic embryo plants", b) protoplast work, c) cryopreservation, d) genetic transformation, and e) artificial or manufactured seed production.

This book discusses basic and applied aspects of somatic embryogenesis, one of the most powerful tools in plant biotechnology. It is divided into three parts; Part I includes topics such as the history of this research field, how differentiated plant cells can (re)acquire totipotency, molecular features, as well as the epigenetics and proteomics of somatic embryogenesis. Part II covers the somatic embryogenesis of different crops, such as Agave spp. maize, Cocos nucifera, Bixa orellana, Capsicum spp., Coffea spp., Musa spp., Pinus spp., and Arabidopsis thaliana. Various applications, like scale-up propagation and genetic engineering are discussed in detail in Part III. The book will appeal to plant scientists, plant breeders and experts working in industry.

The revolutionary progress made in this fascinating field of sexual reproduction inspired this generously illustrated volume. It includes 21 chapters written by experts, covering all aspects of the embryology of angiosperms, ranging from development, isolation, and structure of gametes to endosperm and seed development.