Entomopathogenic Bacteria From Laboratory To Field Application

Entomopathogenic bacteria (Bacillus thuringiensis and B. sphaericus) are increasingly used as biopesticides to control larval insect populations which are either agricultural or forestry pests and to reduce those which as adults are vectors of severe human diseases. This new book, the first since 1993 to address all aspects of entomopathogenic bacteria, provides undergraduate and graduate students as well as research scientists with a complete, modern view of this important group of bacteria. The authors, chosen for their sustained contributions to the field, cover both fundamental and applied research in this area. The main topics include bacterial ecology and taxonomy, toxin diversity, activity and mode of action, regulation and environment of the genes, safety and ecotoxicology, production and field application of the bacteria, and outbreaks of resistant populations. The book concludes with the most recent data obtained on transgenic biotechnology and addresses environmental impact issues.

This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains

This volume provides background theory and practical protocols for bioassays of bacteria, viruses, fungi and nematodes that can be used as biological control agents against insect pests of agricultural and medical importance.

The main scientists working with enhancing fungal, bacterial, virus and insect biological control agents on different targets present the latest progress in overcoming the barrier of insufficient virulence. This multi-disciplinary group review their own work and that of others, and describe the approaches being used, the successes and the barriers yet to be overcome. There is no up-to-date equivalent work describing biocontrol, let alone enhanced biocontrol.

The field of microbial insecticides encompasses the highly diverse life forms bacteria, fungi, nematodes and viruses. They play an essential role in the management of pests in cultivated crops and play a crucial role in the life of farmers and agricultural industry. This book provides a single reference volume with appeal to all levels and fields, including those working in research, teaching, government and industries, assemblages in plant communities.

Mosquitoes and black flies are a constant threat to health and comfort, yet the modern chemical pesticides used to control them have cre ated serious ecological problems. Populations of resistant mosquitoes and black flies have evolved, beneficial insects and natural predators have been destroyed, and environmental pollution has increased worldwide. Therefore, scientists have energetically sought new, environmentally safe technologies to combat mosquitoes and black flies and the diseases they carry. Among the most effective alternative means of controlling these pests are the highly spe cific microbial agents derived from Bacillus tburingiensis or Bacillus spbaericus. The microbial control of mosquitoes and black flies is a very important, rapidly developing area of science. Entomologists and microbiologists have already achieved spectacular successes using B. tburingiensis and B. spbaericus against these pests. Recent discoveries of new bacterial isolates specific to new hosts and recent genetic improvements in these isolates have created the potential for wide-scale use of these biological control agents. Efficient microbial control of mosquitoes and black flies can now be achieved, but a proper knowledge of factors relating to the safe and effective use of these biological control agents is necessary. The efficacy of B. tburingiensis and B. spbaericus is influenced by the inherent differential tol erance of the target mosquitoes or black flies, by the formulation technology and application of these agents, and by environmental factors, especially sun light and temperature.

This book provides basic information and different protocols associated with the Entomopathogenic nematodes (EPNs) and their symbiotic bacteria. Entomopathogenic nematodes (EPNs) of the genera Steinernema and Heterorhabditis and their associated bacterial symbionts Xenorhabdus and Photorhabdus aid nematode infective juveniles (IJs) in infecting and killing their insect hosts, creating a unique tripartite complex of host-vector-symbiont interactions. Due to this insect killing capability, EPNs are used as biological control agents of economically important insect pests. They are also a model system to study host-parasite interactions. It provides a systematic approach to various nematode procedures including pathogenicity, reproduction, foraging behaviour. It gives a brief outline on historical aspects, nematode-bacterium complex, biology and chemical ecology of EPNs. It concisely describes host insect rearing, nematode sampling and storage, isolation techniques, counting, handling and staining of nematodes, characterization including morphological, molecular and ecological studies, mass production, virulence bioassay, field application and efficacy. The book also includes methods and techniques for their associated symbiotic bacteria. This book serves as a laboratory manual and assists the readers to undertake advanced research in different aspects related to nematodes. It is useful for researchers in the fields of nematology, microbiology, bacteriology and entomology.