Enzyme Technology

Today, enzyme technology, amalgamating enzymology with biotechnology, has become a household name in practically all branches of the contemporary science and technology. The book Principles of Enzyme Technology provides an exhaustive presentation of enzyme technology. The text is organised into four parts out of which the first three are more inclined towards imparting the conceptual aspects of the subject, whereas the fourth part accentuates more on the escalating applications of enzymes in industry, be it food, textile or pharmaceutical. Thus, the book offers a balanced insight into the immense world of enzymes in a single readable volume. HIGHLIGHTS OF THE BOOK • Inclusion of a chapter on Enzyme Engineering and Technology makes the book more future-oriented, highlighting the wonders that the modern science can make. • The textual presentation is very lucid, illustrative and organised in a manner that it is not based solely on the complexity of the subject but also on its usefulness. • Adequate number of references, listing of literature for further reading and problems (both multiple choice and thought based) given at the end of each chapter make the book an ideal tool for learning enzyme technology. Primarily intended as a text for the students of biotechnology, biochemistry and other life science branches, this book will be of immense use to the professionals as well as researchers for teaching and references.

Publisher Description

This book gives a broad account of enzymology and aim to put the current knowledge into perspective. The chapters follow a progression from the properties of isolated enzymes to the behaviour of enzymes in increasingly complex systems, leading up to the cell. Included is the discussion on the importance of enzymes in medicine and industry. This book discusses the behaviour of isolated enzymes, dealing in turn with isolation methods, structural characterization, kinetics, catalytic action and control of activity, immobilization methods and various applications of enzymes. The methods for isolation and characterization of enzymes are now well-established procedures, so the rate at which three-dimensional structures and mechanisms are being determined is increasing dramatically. Ultimately it is necessary to know the behaviour of enzymes in living cells. This involves in part a synthesis of the information obtained from the study of isolated enzymes, but it also requires detailed knowledge of the molecular morphology of the cell, which in turn requires methods for making measurements on intact cells. The study and application of enzymes have assumed increasing importance both in medicine and in industry and a discussion of these aspects is therefore given prime importance.

This textbook provides a clear and authoritative guide to the principles and practice of the utilization of enzymes in biotechnology. Enzymes have increasingly important applications in the food and pharmaceutical industry, in medicine, and as biosensors.

Advances in Enzyme Catalysis and Technologies intends to provide the basic structural and functional descriptions, and classification of enzymes. The scientific information related to the recombinant enzyme modifications, discovery of novel enzymes and development of synthetic enzymes are also presented. The translational aspects of enzyme catalysis and bioprocess technologies are illustrated, by emphasizing the current requirements and future perspectives of industrial biotechnology. Several case studies are included on enzymes for biofuels application, micro algal biorefineries, high-value bioactive molecules production and enzymes for environmental processes, such as enzymatic bioprocessing for functional food development, biocatalytic technologies for the production of functional sweetener, etc. Provides a conceptual understanding of enzyme catalysis, enzyme engineering, discovery of novel enzymes, and technology perspectives Includes comprehensive information about the inventions and advancement in enzyme system development for biomass processing and functional food developmental aspects Gives an updated reference for education and understanding of enzyme technology

Enzyme : An Introduction • Enzyme Structure • Enzyme Specificity & Catalysis • Purification & Characterization Of Enzymes • Enzyme Assay • Enzyme Engineering • Enzyme Microenvironment : Catalysis In Non-Aqueous Solvent • Bioenergetics • Introduction To Metabolism • Knzyme Kinetics • Single Substrate Enzyme Inhibition • Kinetics Of Multisubtrate Enzymes • Enzyme'S Regulation And Cooperativity • Enzymes Immbilsation Techniques• Enzyme Biosensor

This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.