Experimental Techniques In Host Plant Resistance

The earliest land-plants evolved around 450 million years ago from aquatic plants devoid of vascular systems. The diversification of flowering plants (angiosperms) during the Cretaceous period is associated with speciation in insects. Early insect herbivores were mandibulate, but the evolution of vascular plants led to the co-evolution of other forms of herbivory, such as leaf feeding, sap-sucking, leaf mining, tissue borer, gall forming and nectar-feeding. Plant defense against biotic stress is an adaptive evolution by plants to increase their fitness. Plants use a variety of strategies to defend against damage caused by herbivores. Plant defense mechanisms are either inbuilt or induced. Inbuilt mechanisms are always present within the plant, while induced defenses are produced or mobilized to the site where a plant is injured. Induced defense mechanisms include morphological, physiological changes and production of secondary metabolites. Host plant resistance (HPR) is one of the eco-friendly methods of pest management. It protects the crop by making it less suitable or tolerant to the pest. While books on theoretical aspects of HPR are available, an exclusive book on the practical aspects is lacking. There is a wide gap between the theory and the experimental procedures required for conducting studies on plant resistance for the post graduate students and young researchers. A dire need for a book on practical aspects was strongly felt. Initially a practical manual was prepared which eventually evolved into the present book. We hope this book provides information on major aspects of screening crop germplasm, sampling techniques, genetic and biochemical basis of HPR, behavioural studies on pheromone and plant volatiles, and some of the recent approaches in HPR. Further, the references provide the scientific articles and books as additional information to readers and workers alike.

The earliest land-plants evolved around 450 million years ago from aquatic plants devoid of vascular systems. The diversification of flowering plants (angiosperms) during the Cretaceous period is associated with speciation in insects. Early insect herbivores were mandibulate, but the evolution of vascular plants led to the co-evolution of other forms of herbivory, such as leaf feeding, sap-sucking, leaf mining, tissue borer, gall forming and nectar-feeding. Plant defense against biotic stress is an adaptive evolution by plants to increase their fitness. Plants use a variety of strategies to defend against damage caused by herbivores. Plant defense mechanisms are either inbuilt or induced. Inbuilt mechanisms are always present within the plant, while induced defenses are produced or mobilized to the site where a plant is injured. Induced defense mechanisms include morphological, physiological changes and production of secondary metabolites. Host plant resistance (HPR) is one of the eco-friendly methods of pest management. It protects the crop by making it less suitable or tolerant to the pest. While books on theoretical aspects of HPR are available, an exclusive book on the practical aspects is lacking. There is a wide gap between the theory and the experimental procedures required for conducting studies on plant resistance for the post graduate students and young researchers. A dire need for a book on practical aspects was strongly felt. Initially a practical manual was prepared which eventually evolved into the present book. We hope this book provides information on major aspects of screening crop germplasm, sampling techniques, genetic and biochemical basis of HPR, behavioural studies on pheromone and plant volatiles, and some of the recent approaches in HPR. Further, the references provide the scientific articles and books as additional information to readers and workers alike.

Most books on epidemiology have treated the subject from a statistical, mathematical or computer applicational point of view. However, experiments must be performed first to provide the data for models which in turn can then be proven by further experimentation. This mutual interplay of theory and empirics gives epidemiology its scientific thrust and charm. This book provides a choice of methods for varying applications and objectives, covering all important aspects for the designing of experiments. Furthermore, the reader is supplied with solutions to his experimental problems and many "tricks of the trade". The newcomer to the field will also profit by this methodology guide.

What is plant resistance to insects? How is plant resistance to insects obtained? How can plant resistance to insects be utilized?

This comprehensive book is the first illustrated volume to provide detailed discussions of all plant genera regarding techniques developed to evaluate plant resistance to insects. Many of the book's references have never before appeared in a volume on this subject. The authors systematically discuss techniques used to evaluate different types of insect behavior and plant morphological and phytochemical factors responsible for plant resistance and susceptibility to insects.

Disease Resistance in Plants, Second Edition, looks at genetic, epidemiologic, biochemical, and biometric principles for developing new cultivars possessing genetic resistance to diseases. It examines the nature of disease resistance and resistance genes, and it highlights the importance of stabilizing selection, sugar, biotrophy, and necrotrophy to obtain the greatest possible yields. Organized into 17 chapters, this volume begins with an overview of disease resistance in plants and the ways to develop disease-resistant variants. It then discusses unspecific resistance; the resistance gene paradox; susceptibility and resistance within narrow host taxa; phenotypic variation and gene numbers in host plants; discontinuous variation and cytoplasmic inheritance; and experimental difficulties in partitioning variance. The reader is also introduced to epistasis and the structure of virulence in pathogens; the notion of physiological race; how the pathogen adapts to the host; mutation in the pathogen from avirulence to virulence; horizontal and vertical resistance to disease and its epidemiological effects; and the link between protein polymorphism and vertical resistance. In addition, the book discusses genes for susceptibility in the host versus genes for avirulence (or virulence) in the pathogen; sink-induced loss of resistance; high-sugar disease processes and biotrophy; slow rusting of cereal crops; plant resistance against endemic disease; and the accumulation of resistance genes in heterogeneous host populations. This book will be useful to plant pathologists and plant breeders.

The 38 chapters of this Field Manual provide the tools required for planning experiments with entomopathogens and their implementation in the field. Basic tools include chapters on the theory and practice of microbial control agents, statistical design of experiments, equipment and application strategies. The major pathogen groups are covered in individual chapters (virus, bacteria, protozoa, fungi, nematodes). Subsequent chapters deal with the impact of naturally occurring and introduced exotic pathogens and inundative application of microbial control agents. The largest section of the Manual is composed of 21 chapters on the application and evaluation of entomopathogens in a wide range of agricultural, forest, domestic and aquatic habitats. Mites and slugs broaden the scope of the book. Supplementary techniques and media for follow-up laboratory studies are described. Three final chapters cover the evaluation of Bt transgenic plants, resistance to insect pathogens and strategies to manage it, and guidelines for evaluating the effects of MCAs on nontarget organisms. Readership: Researchers, graduate students, practitioners of integrated pest management, regulators, those doing environmental impact studies. The book is a stand-alone reference, but is also complementary to the laboratory-oriented Manual of Techniques in Insect Pathology and similar comprehensive texts.