Glycoconjugate Research

Glycoconjugate Research, Volume II contains the proceedings of the Fourth International Symposium on Glycoconjugates held in Woods Hole, Massachusetts in 1977. The papers explore the biosynthesis and regulation of glycoconjugates, particularly those of cell membranes, and glycolipid storage diseases. This volume is comprised of 95 chapters divided into three sections. After reviewing the regulatory mechanisms underlying glycoprotein synthesis, it turns to enzymic modifications of sialic acids in the course of glycoconjugate biosynthesis. The following chapters focus on the biosynthesis and characterization of lipid-linked sugars in the outer membrane of liver mitochondria; effect of bacitracin on the biosynthesis of dolichol derivatives in calf pancreas microsomes; secretion of proteoglycans by chondrocytes; and heterogeneity of arterial proteoglycans. The biosynthesis of elastin by chondroblasts in monolayer cultures is also considered, along with the phosphorylation of proteoglycans in human articular cartilage. The final chapter describes a double-antibody radioimmunoassay for soluble and cell-surface blood group Ii antigens. This book will be a useful resource for biochemists.

Glycoconjugate Research, Volume I contains the proceedings of the Fourth International Symposium on Glycoconjugates, held in Woods Hole, Massachusetts, in September 1977. Contributors focus on the state of knowledge in the field of glycoconjugates ranging from polysaccharides and glycoproteins to glycolipids, proteoglycans, and all their varieties in plants, animals, and microorganisms. This text is organized into three sections and consists of 87 chapters. After an introductory chapter where the use of glycosidases for the structural analysis of complex carbohydrates is described, the discussion turns to other methods used for elucidating the structure of complex carbohydrates. The chapters that follow focus on the primary structure and conformation of glycans that are N-glycosically linked to peptide chains; isolation of monofuco-disialoganglioside from human and bovine brains; and partial chemical characterization of activated peptides isolated from a single cell suspension of rat colonic secretory cells. The last two sections explore cartilage proteoglycans and the structure-function relationships of glycoconjugates, touching on topics such as link proteins; the biochemical aspects of malignancy in human chondrosarcomas; defensive response of plants to a microbial oligosaccharide with a pheromone-like activity; and the interaction of saccharides with ricin. This book is intended for students and practitioners of chemistry and biochemistry; biology and microbiology; and physiology.

Glycoconjugate Research, Volume I contains the proceedings of the Fourth International Symposium on Glycoconjugates, held in Woods Hole, Massachusetts, in September 1977. Contributors focus on the state of knowledge in the field of glycoconjugates ranging from polysaccharides and glycoproteins to glycolipids, proteoglycans, and all their varieties in plants, animals, and microorganisms. This text is organized into three sections and consists of 87 chapters. After an introductory chapter where the use of glycosidases for the structural analysis of complex carbohydrates is described, the discuss...

Glycoconjugates: Advances in Research and Application: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Glycoconjugates in a concise format. The editors have built Glycoconjugates: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Glycoconjugates in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Glycoconjugates: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Recent Trends in Carbohydrate Chemistry: Synthesis and Biomedical Applications of Glycans and Glycoconjugates covers biomedically relevant bacterial cell wall carbohydrates including recent findings on biosynthetic aspects, advances in the chemical assembly of bacterial lipopolysaccharide fragments and teichoic acids, and modern NMR approaches to unravel structural details. The first part introduces and provides the relevant background for synthetic glycoconjugate vaccines. The second section focuses on synthetic carbohydrate-based vaccines of therapeutic potential that are licensed or under development. This second volume of Recent Trends in Carbohydrate Chemistry is ideal for researchers working as synthetic organic chemists, as well as those interested in glycoconjugation, protein chemists, immunologists, and microbiologists, in academia as well as in industry. Highlights important features of bacterial glycoproteins Illustrates modern chemical synthesis and structural analysis of bacterial glycans Demonstrates the importance of carbohydrate chemistry for the synthesis of lipopolysaccharides and teichoic acid Covers recent findings on glycan ligation Gives an overview of the most recent developments on carbohydrate-based vaccines

18. 2 Principle of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. 3 Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 Measurement of Binding Constant for the Interaction Between Protein and Native Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ The Application of Capillary Affinity Electrophoresis to the Analysis _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 Principle of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. 3 Determination of Association Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. 4 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 General considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. 5 Limitations of the Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 Application of CAE to the Analysis of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. 3 Sample Detection and Sample Recovery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and staining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Sample detection by blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. 4 Analysis of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring sample mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical analysis of data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 Interpreting ACE patterns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 Reverse ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. 5 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII List of Contributors Nebojsa Avdalovic John T. Gallagher Dionex Corporation Cancer Research Campaign Department of Medical Oncology 445 Lakeside Drive University of Manchester Sunnyvale, CA 94086 Christie CRC Research Centre Klaus Biemann Wilmslow Road Department of Chemistry Manchester M20 4BX Massachusetts Institute of Technology UK Cambridge, MA 02139-4307 USA Geoffrey R.

Glycotechnology brings together in one place important contributions and up-to-date research results in this fast moving area. Glycotechnology serves as an excellent reference, providing insight into some of the most challenging research issues in the field.

Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.