H P T L C

HPTLC - High Performance Thin-Layer Chromatography

The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.

Chemical Fingerprints of Medicinal Plant- HPTLC Profiling gives an overview of the use of high-performance thin-layer chromatography (HPTLC) for profiling medicinal herbs. Starting with an introduction to HPTLC the book guides the reader through HPTLC instrumentation and laboratory application. Additional notes on the stationary HPTLC phases and hyphenating HTPLC with other analytical techniques are also provided. The book concludes with an exhaustive list of HPTLC fingerprints of many herbs that can be used in medicinal preparations. The book outlines a scientific approach to the identification and selection of herbs for preparing traditional medications. By reading this book, learners will be able to 1) understand the basics of HPTLC, 2) learn how to use the instruments for lab analysis and 3) quickly identify chemical fingerprints of important medicinal herbs. Key Features - A concise overview of HPTLC and associated techniques - A Step-by-step guide to performing HPTLC analysis - Full color illustrations - An exhaustive list of HPTLC fingerprints for 108 medicinal plants, with botanical names - An appendix of TLC solvent systems and reagents for different phytochemical compounds and secondary metabolites - Simple language for easy understanding - Detailed bibliography Chemical Fingerprints of Medicinal Plant- HPTLC Profiling is an essential handbook for scholars in analytical chemistry, plant science and traditional medicine who want to understand the application of HPTLC techniques for research and development in phytomedicine. Readership Scholars in analytical chemistry, plant science and traditional medicine who want to understand the application of HPTLC techniques in phytomedicinal research and development.

High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products. The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as: Am I paying for a high-quality material, but getting a cheap adulterant? Is this raw material worth its price? Does this product comply with the claim on its label? Has the composition of this product changed after being on the shelf for more than a year? Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts, and easy-to-reference text boxes provide summaries of key information.This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs.

Scientific Study from the year 2014 in the subject Biology - Micro- and Molecular Biology, , course: Post Doctoral Research Work, language: English, abstract: Tylophora indica (family Asclepeadaceae) is experimental plant that has been used in the present study. The leaves and roots of Tylophora indica have emetic, cathartic, laxative, expectorant, diaphoretic and purgative properties. It has also been used for the treatment of allergies, cold, dysentery, hay fever and arthritis. It has reputation as an alterative and as a blood purifier, often used in rheumatism and syphilitic rheumatism. Root or leaf powder is used in diarrhea, dysentery and intermittent fever. Dried leaves are emetic diaphoretic and expectorant. It is regarded as one of the best indigenous substitute for ipecacuanha. It is traditionally used as a folk remedy in certain regions of India for the treatment of bronchial asthma, inflammation, bronchitis, allergies, rheumatism and dermatitis. It also seems to be a good remedy in traditional medicine as anti-psoriasis, seborrhea, anaphylactic and leucopenia. The experimental plant of Tylophora indica was procured from Kelkar farm house, Mulund, Mumbai, India (The latitude coordinate of Mumbai is 18 degree 58' 30 North and longitude coordinate is 72 degree 49' 32" East) in the month of April. It has many secondary metabolite viz Tylophorin,Kaempferol and Stigmasterol. Among them tylophorin, an anti asthmatic and anti cancerous phenanthro indolizidine alkaloid is the main constituent of Tylophora indica. The experiments related to Phytochemical studies, Biotechnological studies, Pharmacological studies, BioEnhancement of Chief secondary metabolites and Microbiological studies were carried out which have given the significant results. The Book covers the various aspects and devided into following chapters 1. About Experimental plant 2. BioChemical studies 3. Pharmacological studies 4. Biotechnological studies 5. Enhancement of Secondary metabolites in tissue culture 6. Microbiological studies. Book is suffiecient to illustrate a broad spectrum of various protocols that have been used in all experiments , can be followed by reader easily.