Handbook Of Size Exclusion Chromatography And Related Techniques

Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

The column is the heart of a chromatographic system. For the first time, complete and authoritative information on columns for size exclusion and gel filtration chromatography is available in a single volume containing contributions from every major manufacturer worldwide and many renowned column users. This comprehensive handbook covers the technology, characterization, application, evaluation, maintenance, and quality control of commercial analytical and preparative columns for SEC, GFC, and two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Experienced column users discuss various applications in organic and aqueous mobile phases for synthetic and biopolymers, small solutes, biopharmaceutical therapeutics, and high-temperature SEC.

Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

This work details the practical use of size exclusion chromatography (SEC) in characterizing the molecular weight distribution of important polymeric materials. It addresses problems encountered in the SEC of specific substances, including copolymers, polyamides, polyvinyl alcohol and acetate, lignin derivatives, proteins and starch.

This work details the practical use of size exclusion chromatography (SEC) in characterizing the molecular weight distribution of important polymeric materials. It addresses problems encountered in the SEC of specific substances, including copolymers, polyamides, polyvinyl alcohol and acetate, lignin derivatives, proteins and starch.

Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA. This book... •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems ·Provides practical, hands-on advice based on years of experience ·Will help ensure optimal design, equipment and separation results for your particular task ·Presents system layouts from laboratory to process scale ·Will help you to devise or improve record-keeping and documentation systems

Compiled by the editor of Dekker's distinguished Chromatographic Science series, this reader-friendly reference is as a unique and stand-alone guide for anyone requiring clear instruction on the most frequently utilized analytical instrumentation techniques. More than just a catalog of commercially available instruments, the chapters are wri