Histophatologic Techniques

This book provides a concise definition of the fundamental principles and tech-niques involved in histotechnology. A chapter on Laboratory Risk Management emphasizes the importance of instrumentation, quality control and safety precautions in the working place. The methods involved in tissue processing, histologic staining and cytology are discussed. The use of microwave as a non-chemical way of tissue stabilization and fixation, rapid tissue processing, archival material, enzyme histochemistry and molecular biological techniques such as in-situ hybridization and polymerase chain reaction techniques are described. This book also includes a brief course in immunology, covering antigen-antibody reactions, and the required steps in immunostaining.

This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

This book is a complete guide to histopathology techniques for trainees. Beginning with an introduction to tissue examination, the next chapters discuss fixation and fixatives, tissue processing and embedding, decalcification, microtomy and section cutting, and frozen section and cryostat. The following sections cover different staining procedures, immunohistochemistry, and automation in histopathology, concluding with chapters on biological waste management and quality management. Each chapter includes a self-assessment exercise with short notes and answers, and the comprehensive text is further enhanced by nearly 350 clinical photographs, diagrams and tables. Key points Complete guide to histopathology techniques for trainees Provides detail on different staining procedures, immunohistochemistry, and automation Features self-assessment exercises with notes and answers Highly illustrated with clinical photographs, diagrams and tables

ABOUT THE AUTHORShortly after graduating from the College of Medicine, University of Santo Tomas in Manila Philippines, Dr. Jocelyn H. Bruce-Gregorios (a.k.a. Dr. Joy Bruce) was appinted as a faculty member at De Ocampo Memorial School's Medical Technology Department and later joined the Departments of Physiology and Pharmacology at the University of Santo Tomas Faculty of Medicine and Surgery. She wrote the first edition of "Histopathologic Techniques" before migrating to the United States in 1975. She completed three years of residency training in Anatomic Pathology, one year of clinical training in Neurology, and three years of Neuropathology Fellowship training at the Cleveland Metropolitan General Hospital in Ohio with subspecialty in Electron Microscopy at the Cleveland Clinic. She worked as neuropathologist at Jackson Memorial Hospital in Miami, became a tenured Full Professor at the University of Miami-Miller School of Medicine, and taught pathology residents, medical students and histotechnologists for more than 25 years before retiring in Florida. She was co-author of several Pathology and Neurology book chapters, has given numerous formal presentations at scientific conventions in the U.S., and has published more than ABOUT THE BOOKThis book provides a concise presentation of the fundamental knowledge and principles of histopathologic techniques, and includes new technological methods in histotechnology. A chapter on Risk Management and Safety in the Laboratory emphasizes the importance of instrumentation, quality control and safety precautions when dealing with histologic material. Chapters on histochemistry, rapid processing techniques, and cytology serve as a guide in processing and staining specimen. The use of microwave as a non-chemical method of tissue stabilization and fixation, rapid tissue processing, archival material, immunohistochemistry and molecular biological techniques such as in-situ hybridization and polymerase chain reaction techniques are presented. This book also includes a brief course in immunology, covering topics of antigens, antibodies, antigen-antibody reactions, and an explanation of required steps in immunostaining procedures, introduced at a level which assumes the participant has no previous knowledge of chemistry or immunology.

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

In this manual, author has included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. [Ed.].

本书内容全面,涵盖了组织学技术的方方面面,既包括传统的常用技术,又介绍了近年来新发展的,有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术;对于结缔组织、糖类、脂类、蛋白质、核酸、色素及矿物质、淀粉、神经内分泌颗粒、微体、骨等相关的组绢学技术也予专门章节讲述;另外还涉及了显微镜样品定量、实验室安全及管理,以及人体工程学等相关内容。