Introduction To Plant Biotechnology 2e

Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.

This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.

Focused on basics and processes, this textbook teaches plant biology and agriculture applications with summary and discussion questions in each chapter. Updates each chapter to reflect advances / changes since the first edition, for example: new biotechnology tools and advances, genomics and systems biology, intellectual property issues on DNA and patents, discussion of synthetic biology tools Features autobiographical essays from eminent scientists, providing insight into plant biotechnology and careers Has a companion website with color images from the book and PowerPoint slides Links with author's own website that contains teaching slides and graphics for professors and students: http://bit.ly/2CI3mjp

PART I PLANT GENOME ORGANIZATION NUCLEAR GENOME CHLOROPLAST AND ITS GENOME MITOCHONDRION AND ITS GENOME TRANSPORTABLE ELEMENTS Part II PLANT GROWTH AND REGULATION OF GENE EXPRESSION GROWTH HORMONES REGULATION OF GENE EXPRESSION DURING PLANT DEVELOPMENT PHYTOCHROMESEED STORAGE PROTEINSINDUCIBLE CONTROL OF GENE EXPRESSION PART III APPLIED PLANT BIOTECHNOLOGY PLANT TISSUE CULTURE TRANSGENIC PLANTS MOLECULAR PHARMING Review Questions Glossary References Index

Plant biotechnology plays a very important role in basic and applied sciences. It is a scientific technique that adapts plants for specific purposes of cross-breeding, extending their growing seasons, adjusting height, colour, and texture, and several other mechanisms. Plant biotechnology helps plant breeders to develop crops with specific beneficial and desirable traits. Thus, it has emerged as an important aspect of agriculture. Plant Biotechnology comprehensively covers different aspects based on the latest outcomes of this field. Topics such as tissue culture, nutrient medium, micronutrients, macronutrients, solidifying agents/supporting systems, and growth regulators have been dealt with extensively. The book also discusses in detail plant genetic engineering for productivity and performance, resistance to herbicides, insect resistance, resistance to abiotic stresses, molecular marker aided breeding, molecular markers, types of markers, and biochemical markers. Different aspects of important issues in plant biotechnology, commercial status and public acceptance, biosafety guidelines, gene flow and IPR have been also thoroughly examined. This book caters to the needs of graduate, postgraduate and researchers.

Plant Biotechnology presents a balanced, objective exploration of the technology behind genetic manipulation, and its application to the growth and cultivation of plants. The book describes the techniques underpinning genetic manipulation and makes extensive use of case studies to illustrate how this influential tool is used in practice.

In vitro Plant Biotechnology: Status and Scope, In vitro Plant Regeneration—An Overview, In vitro Culture Laboratory— Organization and Management, Sterilization Techniques, Plant Cell In vitro Nutrition: Culture Medium, Cell Differentiation and Totipotency, Micropropagation: A Source of Clonal Regeneration, Callus: Induction and Differentiation, Cell Suspension Culture, Single Cell Culture: Technology and Applications, Embryo Culture, Somatic Embryo: Induction and Regeneration, Haploid Production-I (Androgenesis), Haploid Production-II (In vitro Pollination Fertilization and Gynogenesis), Endosperm and Nucellus Culture, Protoplast Technology— Isolation and Regeneration of Protoplast, Protoplast Technology— Somatic Hybridization and Cybridization, Somaclonal Variation: Source and Significance, Biodiversity and Preservation of Germplasm, Artificial (synthetic) Seed Production Technology, Secondary Metabolite Production-I, Secondary Metabolite Production-II, Transgenic Production-I, Transgenic Production-II, Transgenic Production-III, G M Crops and their Impacts, Plastid Engineering, Plant In vitro Biotechnology in Agriculture, Plant In vitro Biotechnology in Forestry, Plant In vitro Biotechnology in Industry.