Introduction To Spatial Mapping Of Biomolecules By Imaging Mass Spectrometry

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Provides the introductory foundations of imaging mass spectrometry for those new to the technique Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work Includes case studies summarizing the imaging MS techniques developed for the class of molecules

The separation of a mixture into its individual components is one of the most fundamental procedures in analytical and industrial chemistry. This classic book in analytical chemistry provides a comprehensive yet systematic outline of all known separation methods. Through its detailed treatment of the basic principles of separation possibilities, it not only covers what is currently known, but also represents a treasure trove of methods that are still awaiting further development. It is clearly structured and contains interesting examples, further reading and a detailed index. An indispensable book for advanced students of natural sciences (chemistry, biochemistry, food chemistry, pharmacy, clinical chemistry, environmental sciences) and technology (chemical engineering, chemical-physical measurement & biotechnology), as well as teachers of these disciplines.

This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.

Mass spectrometry has emerged as a powerful tool for analyzing and identifying biomolecules of interest, including small molecules, pharmaceutical compounds, and tissue biomarkers - such as proteins and glycans. While most mass spectrometry based analytical techniques focus on liquid-chromatography coupled with tandem mass spectrometry for biomolecule analysis, mass spectrometry imaging (MSI) provides a unique advantage to map spatial distribution within an anatomical context of the tissue of interest. We use a high-resolution accurate mass MALDI Orbitrap LTQ XL mass spectrometer, that offers enhanced mass spectral resolution and high accuracy mass measurements critical for molecular identification, to perform MSI on thin tissue sections. In this thesis, I will highlight specific examples where the information gained from imaging is critical to understanding the question of interest: 1) Development of pathology-directed biomarker analysis of clinically relevant tissues 2) Pushing throughput for MSI in vitro drug penetration analysis of three-dimensional cell cultures including spheroid and organoid systems and 3) Analysis of lipids in insulator regions of the electric eel for the development of bioelectric batteries.

This NATO-ASI installment is designed to provide an advanced overview for doctoral and post-doctoral candidates of the state-of-the-art technologies for bio-detection. The main objective of the work aims at providing readers with the latest developments necessary to successfully understand the CBRN Agents and their associated biotechnologies. The core methods focused on are mass spectrometry (including chromatographic and electrophoretic separation) and comparisons to spectroscopic, immunological and molecular analysis of chemical, biological and nuclear agents.

This second edition details new and updated chapters on key methodologies and breakthroughs in the mass spectrometry imaging (MSI) field. Chapters guide readers through nano-Desorption Electrospray Ionisation (nDESI), Matrix Assisted Laser Desorption Ionisation-2 (MALDI-2), Laser Ablation - Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) ,Imaging Mass Cytometry (IMC) with a variety of diverse samples including eye tissue, crop analysis, 3D cell culture models, and counterfeit goods analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Imaging Mass Spectrometry: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

This volume constitutes the proceedings of the 9th International Work-Conference on IWBBIO 2020, held in Maspalomas, Gran Canaria, Spain, in June 2022. The total of 75 papers presented in the proceedings, was carefully reviewed and selected from 212 submissions. The papers cover the latest ideas and realizations in the foundations, theory, models, and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.