Laboratory Practices In Microbiology

Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more. Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms. Gives complete explanations for all steps in experiments, thus helping readers easily understand experimental procedures Includes certain subjects that tend to be disregarded in other microbiology laboratory books, including microorganisms in the environment, pure culture methods, wet-mount and hanging drop methods, biochemical characteristics of microorganisms, osmotic pressure effects on microorganisms, antiseptic and disinfectants effects on microorganisms, and more Provides groupings and characterizations of microorganisms Functions as a representative reference book for the field of microbiology in the laboratory

Ever-increasing public interest and concern over food safety, as well as commercial pressure to improve food quality and extend product shelf life, have greatly increased the responsibility and accountability of all those involved in the microbiological examination of foods and food-related samples. In order to maintain the consistently high standards of laboratory practice that are required in food microbiology, all staff must be suitably trained to understand what they are to do, how they are to do it and why they must do it in a prescribed way. Properly trained laboratory staff are a valuable asset, whether they work in a food industry, public health, research or contract testing laboratory, and they make a significant contribution to the reliability of the results obtained from microbiological examinations of food samples. This book is an essential training aid and reference for all trainees in food microbiology laboratories, as well as their teachers, their trainers and all those attending food microbiology training courses. It provides an up-to-date, comprehensive working knowledge of all areas of basic food microbiology, with particular focus and emphasis on laboratory-based, practical aspects. Information and comment is provided on:- groups of microorganisms of importance in food microbiology: factors affecting the growth, survival and death of microorganisms in foods food spoilage, food-borne illness and food preservation applications of microbiology in the food industry laboratory design, equipment, operation and practice laboratory accreditation, performance monitoring and systems for documentation use of laboratory equipment, basic techniques and obtaining samples conventional methods for microbiological examination confirmation tests and how they work, and an introduction to 'alternative' microbiological methods Each topic is accompanied by further information sources that will help in the development of high standards for the next and future generations of practical food microbiologists. Provides a fully up-to-date working knowledge of all aspects of food microbiology with a particular focus on practical laboratory aspects. Focuses on laboratory methodology and how to get good results.

This new edition includes an update on HIV disease/AIDS, recently developed HIV rapid tests to diagnose HIV infection and screen donor blood, and current information on antiretroviral drugs and the laboratory monitoring of antiretroviral therapy. Information on the epidemiology and laboratory investigation of other pathogens has also been brought up to date. Several new, rapid, simple to perform immunochromatographic tests to assist in the diagnosis of infectious diseases are described, including those for brucellosis, cholera, dengue, leptospirosis, syphilis and hepatitis. Recently developed lgM antibody tests to investigate typhoid fever are also described. The new classification of salmonellae has been introduced. Details of manufacturers and suppliers now include website information and e-mail addresses. The haematology and blood transfusion chapters have been updated, including a review of haemoglobin measurement methods in consideration of the high prevalence of anaemia in developing countries.

Basic methods; Techniques in applied microbiology; Schemes for the identification of micro-organisms; Recipes for stains reagents and media; Probability tables for the estimation of bacterial numbers by the dilution tube technique.

A practical and well-illustrated guide to microbiological, haematological, and blood transfusion techniques.

Anaerobic Bacteriology: Clinical and Laboratory Practice, Third edition discusses the importance of the non-sporing anaerobic bacteria as a significant cause of infection in man. This edition updates the anaerobic methodology, systematics, and ecological and pathogenetic associations of the non-sporing anaerobes. The descriptive bacteriology of the non-clostridial anaerobes and clinical syndromes produced by them in man are also considered. Other topics discussed include the anaerobic jar, inoculation of media, and antibiotic susceptibility testing of anaerobes. The histotoxic clostridia of infected wounds, anaerobic cocci, and infections related to the gastrointestinal tract are also elaborated. This text likewise covers the uterine gas gangrenous infections and other clostridial infections. This book is a good source for medical practitioners, clinicians, and medical students concerned with anaerobic bacteria.