Mesenchymal Stem Cells Basics And Clinical Application Ii

Engineered MSCs from Patient-Specific iPS Cells, by Irina Eberle, Mohsen Moslem, Reinhard Henschler, Tobias Cantz Fate of Intravenously Injected Mesenchymal Stem Cells and Significance for Clinical Application, by Beate Wagner, Reinhard Henschler The Implications of Stem Cell Applications for Diseases of the Respiratory System, by Mei Ling Lim, Philipp Jungebluth, Paolo Macchiarini Potential of Mesenchymal Stem Cell Applications in Plastic and Reconstructive Surgery, by Birgit Weyand, Peter M. Vogt General Principles for the Regeneration of Bone and Cartilage, by Michael Jagodzinski, C. Haasper Adult Mesenchymal Stem Cells Explored in the Dental Field, by K. M. Fawzy El-Sayed, C. Dörfer, F. Fändrich, F. Gieseler, M. H. Moustafa, H. Ungefroren Mesenchymal Stem Cell Therapy and Lung Diseases, by Khondoker M. Akram, Sohel Samad, Monica Spiteri, Nicholas R. Forsyth Mesenchymal Stem Cells as Cellular Immunotherapeutics in Allogeneic Hematopoietic Stem Cell Transplantation, by Claudia Papewalis, Daniela Topolar, Barbara Götz, Stefan Schönberger, Dagmar Dilloo New Cell-Based Therapy Paradigm: Induction of Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells into Pro-Inflammatory MSC1 and Anti-inflammatory MSC2 Phenotypes, by Aline M. Betancourt Interactions Between Mesenchymal Stem Cells and Dendritic Cells, by Grazia Maria Spaggiari, Lorenzo Moretta MSC and Tumors: Homing, Differentiation, and Secretion Influence Therapeutic Potential, by Naomi D’souza, Jorge Sans Burns, Giulia Grisendi, Olivia Candini, Elena Veronesi, Serena Piccinno, Edwin M. Horwitz, Paolo Paolucci, Pierfranco Conte, Massimo Dominici Sources of Mesenchymal Stem Cells: Current and Future Clinical Use, by Michela Pozzobon, Martina Piccoli, Paolo De Coppi Role of the EU Framework in Regulation of Stem Cell-Based Products, by Giovanni Migliaccio, Cristina Pintus

Prospective Isolation and Characterization of Human Bone Marrow-Derived MSCs, by A. Harichandan, K. Sivasubramaniyan, H.-J. Bühring Urine as a Source of Stem Cells, by Christina Benda, Ting Zhou, Xianming Wang, Weihua Tian, Johannes Grillari, Hung-Fat Tse, Regina Grillari-Voglauer, Duanqing Pei, Miguel A. Esteban Expansion of Mesenchymal Stem/Stromal Cells under Xenogenic-Free Culture Conditions, by Sven Kinzebach, Karen Bieback Adipose-Derived Mesenchymal Stem Cells: Biology and Potential Applications, by Danielle Minteer, Kacey G Marra, J Peter Rubin Potential for Osteogenic and Chondrogenic Differentiation of MSC, by Antonina Lavrentieva, Tim Hatlapatka, Anne Neumann, Birgit Weyand, Cornelia Kasper Potential for Neural Differentiation of Mesenchymal Stem Cells, by Letizia Ferroni, Chiara Gardin, Ilaria Tocco, Roberta Epis, Alessandro Casadei, Vincenzo Vindigni, Giuseppe Mucci, Barbara Zavan Migratory Properties of Mesenchymal Stem Cells, by Thomas Dittmar, Frank Entschladen Dissecting Paracrine Effectors for Mesenchymal Stem Cells, by Stefania Bruno, Federica Collino, Ciro Tetta, Giovanni Camussi Proteomics Approaches in the Identification of Molecular Signatures of Mesenchymal Stem Cells, by Yin Xiao, Jiezhong Chen Does the Adult Stroma Contain Stem Cells?, by Richard Schäfer

Over the past decade, significant efforts have been made to develop stem cell-based therapies for difficult to treat diseases. Multipotent mesenchymal stromal cells, also referred to as mesenchymal stem cells (MSCs), appear to hold great promise in regards to a regenerative cell-based therapy for the treatment of these diseases. Currently, more than 200 clinical trials are underway worldwide exploring the use of MSCs for the treatment of a wide range of disorders including bone, cartilage and tendon damage, myocardial infarction, graft-versus-host disease, Crohn’s disease, diabetes, multiple sclerosis, critical limb ischemia and many others. MSCs were first identified by Friendenstein and colleagues as an adherent stromal cell population within the bone marrow with the ability to form clonogenic colonies in vitro. In regards to the basic biology associated with MSCs, there has been tremendous progress towards understanding this cell population’s phenotype and function from a range of tissue sources. Despite enormous progress and an overall increased understanding of MSCs at the molecular and cellular level, several critical questions remain to be answered in regards to the use of these cells in therapeutic applications. Clinically, both autologous and allogenic approaches for the transplantation of MSCs are being explored. Several of the processing steps needed for the clinical application of MSCs, including isolation from various tissues, scalable in vitro expansion, cell banking, dose preparation, quality control parameters, delivery methods and numerous others are being extensively studied. Despite a significant number of ongoing clinical trials, none of the current therapeutic approaches have, at this point, become a standard of care treatment. Although exceptionally promising, the clinical translation of MSC-based therapies is still a work in progress. The extensive number of ongoing clinical trials is expected to provide a clearer path forward for the realization and implementation of MSCs in regenerative medicine. Towards this end, reviews of current clinical trial results and discussions of relevant topics association with the clinical application of MSCs are compiled in this book from some of the leading researchers in this exciting and rapidly advancing field. Although not absolutely all-inclusive, we hope the chapters within this book can promote and enable a better understanding of the translation of MSCs from bench-to-bedside and inspire researchers to further explore this promising and quickly evolving field.

This book provides a comprehensive review of the properties of various stem cell types, the mechanisms of their behaviors and their potential clinical application. Stem cells have a great capacity of self-renewal and differentiation. They represent new paradigms for disease treatment in the field of regenerative medicine since the day they were discovered. As stem cell research is complicated and making progress rapidly, it is important to have expertise in this field to share their views and perspectives. This book provides a wonderful platform for those who are interested in stem cells to learn from and communicate with experts. Particularly, it highlights the roles of stem cell based therapy for a variety of diseases. Furthermore, this book gives a detailed introduction to the great works related to stem cells in China. The readers could gain a profound knowledge of the state-of-art research done by scientists in the field of stem cells. Overall, this book will be a valuable reference resource for both experienced investigators pursuing stem cell research as well as those are just entering into this field. Dr. Robert Chunhua Zhao, a Cheung Kong Professor of Stem Cell Biology, is Professor of Cell Biology at the Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College (PUMC), Beijing, China. He is Director of the Center for Tissue Engineering, PUMC and Chief Scientist of the National Basic Research Program of China (“973 Program”). He also serves as Regional Editor of Stem Cells and Development.

Mesenchymal stem cells (MSCs), a type of adult stem cells, have attracted the attention of scientists and physicians alike due to their unique biological properties and potential for disease treatment. As stem cell research is complex and progressing rapidly, it is important that the experts in this field share their views and perspectives. This book, co-edited by leading global researchers, is divided into three major sections and covers a broad range of topics concerning MSCs during their transition from benchside to bedside. The book is intended for researchers and clinicians in the field of stem cells. Dr. Robert Chunhua Zhao, MD. Ph.D is Cheung Kong Professor of Stem Cell Biology, Professor of Cell Biology at the Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, China Director of Center for Tissue Engineering, PUMC Chief scientist of 973 program Regional Editor of Stem Cells and Development.

This book focuses on mesenchymal stem cells (MSCs) of animal origin, including their isolation, characterization, and clinical applications. After briefly discussing the historical development of the field of stem cell research, it describes the basic properties and nature of stem cells particularly in relation to MSCs. In turn, it reviews materials and methods used to isolate MSCs from various sources, culture expansion, characterization and long-term storage. It also explores the therapeutic efficacy, immunomodulation and anti-inflammatory, and differentiation properties of MSCs. Importantly, the book discusses the applications of genetic engineering to enhance the efficacy and potential of MSCs in regenerative medicine. The book largely addresses the potential applications of mesenchymal stem cells in therapies for important species of domesticated animals including sheep, goats, cattle, buffalo, cats, dogs and horses. Finally, the book presents an abridgement of challenges and future prospects of stem cell research and application in medicine, in general and veterinary sciences, in particular.

Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types such as bone cells, cartilage cells, muscle cells and fat cells. They are also called medicinal signaling cells. MSCs are characterized by plastic adherent properties under normal culture conditions and a fibroblast-like morphology. Differentiation capacity, immunomodulatory effects and antimicrobial poperties are a few functions of mesenchymal stem cells. Their clinical applications include muscle repair and treatment of auto-immune diseases. From theories to research to practical applications, case studies related to all contemporary topics of relevance to mesenchymal stem cells have been included in this book. Different approaches, evaluations, methodologies and advanced studies on this field have been included herein. This book is a vital tool for all researching or studying mesenchymal stem cells as it gives incredible insights into emerging trends and concepts.