Microorganisms And Enzymes For Lignocellulosic Biorefineries

Enzymes and Microorganisms for Lignocellulosic Biorefinery comprehensively deals with the enzymes and microorganisms for lignocellulosic degradation, challenges in the engineering of lignocellulolytic enzymes and mining and engineering for better enzymes. The book discusses commonly used bioprocesses for lignocellulosic biorefinery, including separated hydrolysis and fermentation, simultaneous saccharification and fermentation and consolidated bioprocessing. Among these methods, construction of microbial co-culturing systems via consolidated bioprocessing is regarded as a potential strategy to efficiently produce biochemicals and biofuels, providing theoretical direction for constructing efficient and stable biorefinery process system in the future. The book discusses construction of high-performance enzyme cocktails, and presents progress witnessed in engineering lignocellulolytic enzymes and enzyme-producing microorganisms and future perspectives in the context of developing cost-effective lignocellulose conversion processes. Presents drivers for biorefinery industry development Discusses global drivers towards the advancements of lignocellulosic biorefineries, technical and operational challenges for industrialization towards overcoming them Discusses the biorefinery value chain, its economical, and technical considerations Provides SWOT analysis and future directions

This volume summarizes our current knowledge on different biomass-converting enzymes and their potential use in converting biomass into simple sugar to generate bioenergy and other value added co-/by products. It consists of 13 chapters and is divided into three parts: cellulases; hemicellulases; and lignocellulose oxidoreductases. Written by international experts, the contributions offer clear and concise descriptions of both standard and new technologies. It is an invaluable reference resource for undergraduates, post-graduates, researchers and practitioners in the field of microbial enzymes for biofuel and biorefinery applications.

The book describes how plant biomass can be used as renewable feedstock for producing and further processing various products. Particular attention is given to microbial processes both for the digestion of biomass and the synthesis of platform chemicals, biofuels and secondary products. Topics covered include: new metabolic pathways of microbes living on green plants and in silage; using lignocellulosic hydrolysates for the production of polyhydroxyalkanoates; fungi such as Penicillium as host for the production of heterologous proteins and enzymes; bioconversion of sugar hydrolysates into lipids; production of succinic acid, lactones, lactic acid and organic lactates using different bacteria species; cellulose hydrolyzing bacteria in the production of biogas from plant biomass; and isoprenoid compounds in engineered microbes.

The agricultural and forestry processing wastes (lignocellulosics) are an important material resource and energy source. However, if untreated they can pose a danger to the environment and potentially valuable resources. Microorganisms contribute significantly to solving the problem of biomass degradation, its recycling and conservation. In the recent years, an increasing interest shown by the textile, food, feed & pulp, and paper industries in the microbial and enzymatic processes has triggered in-depth studies of lignocellulolytic microorganisms and their enzymes. Moreover, the advent of recombinant DNA technology in the late 1970s further paved the way for developing technologies based on lignocellulolytic microbes and enzymes. Lignocellulose Biotechnology presents a comprehensive review of the research directed towards environmentally friendly agricultural and forest by-products. The book comprises 22 chapters, divided in four sections. It deals with a wide range of topics including biodiversity of lignocellulose degrading microorganisms and their enzymes, molecular biology of biodegradation of lignin, characterization of lignocellulolytic enzymes, bioconversion of plant biomass to produce enzymes, animal feed, bioethanol and industrial applications of lignocellulolytic enzymes. The chapters dealing with industrial applications also address current biotechnological approaches in lignocellulose bioconversion to value added products. This book is essential for students, researchers, scientists, and engineers working in the fields of environmental microbiology, environmental biotechnology, life sciences, waste management, and biomaterials.

Bioethanol has been recognized as a potential alternative to petroleum-derived transportation fuels. Even if cellulosic biomass is less expensive than corn and sugarcane, the higher costs for its conversion make the near-term price of cellulosic ethanol higher than that of corn ethanol and even more than that of sugarcane ethanol. Conventional process for bioethanol production from lignocellulose includes a chemical/physical pre-treatment of lignocellulose for lignin removal, mostly based on auto hydrolysis and acid hydrolysis, followed by saccharification of the free accessible cellulose portions of the biomass. The highest yields of fermentable sugars from cellulose portion are achieved by means of enzymatic hydrolysis, currently carried out using a mix of cellulases from the fungus Trichoderma reesei. Reduction of (hemi)cellulases production costs is strongly required to increase competitiveness of second generation bioethanol production. The final step is the fermentation of sugars obtained from saccharification, typically performed by the yeast Saccharomyces cerevisiae. The current process is optimized for 6-carbon sugars fermentation, since most of yeasts cannot ferment 5-carbon sugars. Thus, research is aimed at exploring new engineered yeasts abilities to co-ferment 5- and 6-carbon sugars. Among the main routes to advance cellulosic ethanol, consolidate bio-processing, namely direct conversion of biomass into ethanol by a genetically modified microbes, holds tremendous potential to reduce ethanol production costs. Finally, the use of all the components of lignocellulose to produce a large spectra of biobased products is another challenge for further improving competitiveness of second generation bioethanol production, developing a biorefinery.

This reference book provides advanced knowledge about lignocellulosic biomass production and its application in biomass hydrolysis. Lignocellulosic biomass is the most abundant, ubiquitous, and renewable raw material in the world. Though biomass can be deconstructed by other means, biological ways through enzymes are eco-friendly and sustainable. Biomass Hydrolyzing Enzymes: Basics, Advancements, and Applications discusses the different enzymes used for degrading biomass into its monomeric components. It covers important topics like biorefineries, hydrolysis of algal mass, kinetic modelling for hydrolysis, inhibitory effects, and more. Key Features Highlights recent developments in biorefineries, specific enzymes, inhibitor tolerance, and enhanced efficiencies Provides details on various kinds of biomass hydrolysis including algal biomass Includes the best practices for getting economic and efficient high conversions of biomass Covers strategies to be adopted for increasing the production of highly efficient enzymes Explores the advancements in lignocellulosic biomass hydrolysis The book is suitable for researchers and students in biotechnology, applied microbiology, and environmental sciences.

This book offers a complete introduction for novices to understand key concepts of biocatalysis and how to produce in-house enzymes that can be used for low-cost biofuels production. The authors discuss the challenges involved in the commercialization of the biofuel industry, given the expense of commercial enzymes used for lignocellulose conversion. They describe the limitations in the process, such as complexity of lignocellulose structure, different microbial communities’ actions and interactions for degrading the recalcitrant structure of lignocellulosic materials, hydrolysis mechanism and potential for bio refinery. Readers will gain understanding of the key concepts of microbial catalysis of lignocellulosic biomass, process complexities and selection of microbes for catalysis or genetic engineering to improve the production of bioethanol or biofuel

This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.