Molecular Diagnosis And Epidemiology Of Human Pathogens

Medical Microbiology deals with the study of microorganisms that colonize or infect humans. It studies the pathogenesis and epidemiology of these microorganisms with practical applications in diagnosis, treatment and prevention of diseases (infectious diseases, infection-related cancer, dysbiosis related diseases, etc.,). In the past two decades, its expansion is essentially due to the great advances in related fields such as immunology, genetics, bioinformatics, science, technology, engineering and mathematics. New scientific knowledge and understanding make possible new applications. Nevertheless, it becomes a challenge due to lack of expertise to analyze fast-growing data and to translate them in clinical practice and medical education. The pace and sophistication of advances in all scientific disciplines applied to medical microbiology call for a comprehensive and integrative understanding of the overall knowledge development in the different related fields (immunology, genetics, bioinformatics, science, technology, engineering and mathematics, etc.,). In light of this, the main goal of this topical collection is to bridge the gap between all these fields. Each article in this compilation is a stand‐alone text based on an integrative review, which highlights advances in molecular diagnosis and epidemiology of human pathogens. It also helps researchers and practitioners to access and scrutinize the fast expanding knowledge. This research topic in medical microbiology provides an updated core of basic knowledge critical to clinical practice in medicine. It is designed for physicians, researchers, educators, residents, Lab technicians, undergraduate and graduate students. Each article deals with an important human pathogen. It uses a multidisciplinary approach integrating new advances, especially in genetics, bioinformatics, science, technology, engineering and mathematics. It provides an update of the current state-of-the-art scientific knowledge applied to molecular diagnosis and epidemiology of human pathogens. Rapid detection, accurate identification and typing of causative pathogens are necessary to optimize treatment, to trace pathogens and to study microbial population dynamics.

The first in a series of four books, this comprehensive work details the molecular detection of major human viral, bacterial, fungal, and parasitic pathogens. Focusing on sample preparation and molecular detection procedures, each chapter presents a concise review of the pathogen concerned including its taxonomy, biology, epidemiology and pathogenesis; a description of clinical sample collection preparation procedures; a summary of molecular detection methods; representative molecular detection protocols; and a discussion on the challenges, limitations, and advantages for the current methods as well as further research required to improve diagnosis.

In a unique way, the laboratory professionalist's knowledge about the molecular diagnostics of infectious diseases is connected with the clinician's practical experience. The increasing prevalence of infectious diseases makes correct diagnosis, ordering the right tests, quality control and correct interpretation of the results even more important. The possible pathogens are discussed in the second part according to the specific symptoms or to where the infection is located in the body. For clinicians and laboratory professionalists Up-to-date diagnostics of all infectious diseases Molecular diagnostics, diseases, choice of methods Specific pathogens in alphabetic order Tables and flow charts

Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites. The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens. This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes: A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.

The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains. A wide range of techniques has been applied to the investigation of outbreaks of tra- missible disease, and these have been critical in unraveling the route of spread of pat- gens for humans, animals, and plants. The choice of a molecular method will depend on the type of questions to be addressed, on the degree of genetic diversity of the species to be analyzed, and on the mechanisms responsible for generation of the diversity. The applications of molecular methods, singly or in combination, have greatly contributed in the past two decades to basic microbial science and public health control strategies. Molecular Epidemiology of Microorganisms: Methods and Protocols brings together a series of methods-based chapters with examples of application to some of the most important microbes. Both traditional and novel techniques are described, and the type of information that can be expected to be obtained by their application is indicated.

Molecular Medical Microbiology was the first book to synthesise the many new developments in both molecular and clinical research in a single comprehensive resource. The molecular age has brought about dramatic changes in medical microbiology, and great leaps in our understanding of the mechanisms of infectious disease. This 3rd edition of Molecular Medical Microbiolog 3rd , completely updated, reviewed, and expanded with an extra volume will again be the must-have on every shelf of every hospital lab and university department. Molecular Medical Microbiology, 3rd is a very timely and helpful update for microbiologists, students and clinicians in the ear of increasing use of molecular techniques, changing epidemiology and prevalence and increasing resistance of many pathogenic bacteria. Written by experts in the field, chapters include cutting edge information, and clinical overviews for each major bacterial group, in addition to the latest updates on vaccine development, molecular technology and diagnostic technology and expanded with an extra 4th volume dedicated to virology. Completely updated, revised comprehensive and accessible reference on molecular medical microbiology Full color presentation throughout In-depth discussion of individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology, and diagnostic technology More than 100 chapters covering all major groups of bacteria Written by an international panel of authors who are experts in their respective disciplines

This updated second edition of Molecular Typing in Bacterial Infections, presented in two volumes, covers both common and neglected bacterial pathogenic agents, highlighting the most effective methods for their identification and classification in the light of their specific epidemiology. New chapters have been included to add new species, as well as another view of how bacterial typing can be used. These books are valuable resources for the molecular typing of infectious disease agents encountered in both research and hospital clinical laboratory settings, as well as in culture collections and in the industry. Each of the 21 chapters provides an overview of specific molecular approaches to efficiently detect and type different bacterial pathogens. The chapters are grouped in five parts, covering respiratory and urogenital pathogens (Volume I), and gastrointestinal and healthcare-associated pathogens, as well as a new group of vector-borne and Biosafety level 3 pathogens including a description of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology (Volume II). Comprehensive and updated, Molecular Typing in Bacterial Infections provides state-of-the-art methods for accurate diagnosis and for the correct classification of different types which will prove to be critical in unravelling the transmission routes of human pathogens.

While the vast majority of our food supplies are nutritious and safe, foodborne pathogen-related illness still affects millions of people each year. Large outbreaks of foodborne diseases — such as the recent salmonella outbreak linked to various peanut butter products — continue to be reported with alarming frequency. All-Encompassing Guide to Detection Techniques One of the most effective ways to control and prevent human foodborne infections is to implement a pathogen surveillance system. Addressing the biology, epidemiology, and pathogenesis of more than 60 microorganisms, Molecular Detection of Foodborne Pathogens demonstrates how to implement a surveillance system that uses state-of-the-art molecular techniques to quickly detect and identify bacterial, fungal, viral, and parasitic pathogens concerned. Included in each chapter: Concise review of the selected pathogen in respect to its biology, epidemiology, and pathogenesis Summary of molecular detection methods available Description of clinical/food sample collection and preparation procedures Selection of robust, effective, step-by-step detection protocols Discussion of the current challenges and ongoing research needs to further extend utility and performance of molecular diagnostic methods With proven, ready-to-use protocols (including commercial kits), this globally pertinent resource demonstrates how speedy and effective detection methods can lead to more lives saved and a consistently safe, quality, and trustworthy food supply.