Neuroscience Protocols

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.

Short Protocols in Neuroscience: Systems and Behavioral Methods provides a portable and streamlined at-the-bench resource of systems and behavorial methods from the acclaimed Current Protocols in Neuroscience. It covers areas such as electrophysiological analysis of neural cells, the chemistry and pharmacology of the nervous system, behavioral analysis, and animal models of neurologic and psychiatric disorders.

Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step

AACN Protocols for Practice: Monitoring Neuroscience Patients provides clinicians at the point of care with the latest research findings in patient care in a format which is easy to understand and integrate into clinical practice. Each protocol guides clinicians in the appropriate selection of patients, use and application of management principles, initial and ongoing monitoring, discontinuation of therapies or interventions, and selected aspects of quality control.