Nonisotopic Immunoassay
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Nonisotopic Immunoassay
Author | : That T. Ngo |
Publsiher | : Springer Science & Business Media |
Total Pages | : 487 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 9781468454666 |
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The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.
The Immunoassay Handbook
Author | : David Wild |
Publsiher | : Elsevier |
Total Pages | : 968 |
Release | : 2005-06-20 |
Genre | : Medical |
ISBN | : 0080445268 |
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Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
Ligand Assay
Author | : John Langan,Jeremy J. Clapp |
Publsiher | : Unknown |
Total Pages | : 286 |
Release | : 1981 |
Genre | : Biochemistry |
ISBN | : 0893520942 |
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Nonisotopic Alternatives to Radioimmunoassay
Author | : Lawrence A. Kaplan,Amadeo J. Pesce |
Publsiher | : Unknown |
Total Pages | : 368 |
Release | : 1981 |
Genre | : Medical |
ISBN | : UOM:39015000795982 |
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Advances in Clinical Chemistry
Author | : Anonim |
Publsiher | : Academic Press |
Total Pages | : 319 |
Release | : 1985-11-20 |
Genre | : Science |
ISBN | : 9780080566221 |
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Advances in Clinical Chemistry
Non Isotopic Immunoassays and Their Applications
Author | : G. P. Talwar |
Publsiher | : Stosius Incorporated/Advent Books Division |
Total Pages | : 436 |
Release | : 1983-06-01 |
Genre | : Electronic Book |
ISBN | : 0706919009 |
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Immunochemistry of Solid Phase Immunoassay
Author | : John E. Butler |
Publsiher | : CRC Press |
Total Pages | : 334 |
Release | : 1991-07-23 |
Genre | : Science |
ISBN | : 0849353947 |
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Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.
Enzyme Immunoassay
Author | : Edward T. Maggio |
Publsiher | : CRC Press |
Total Pages | : 305 |
Release | : 2018-01-18 |
Genre | : Health & Fitness |
ISBN | : 9781351080293 |
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The purpose of this book is to focus attention on some of these ideas and concepts. In doing so, it has captured a glimpse of the past and it attempts a projection of the future, but mostly it reveals an overview of the field as it exists as the present time. It aims to serve to spawn further growth in ideas and encourage applications to increasingly broader segments of both clinical and general analytical chemistry fields.