Practical Plant Virology

Viruses require a special approach to establish their presence in a diseased plant since they are not visible, even under a light microscope. This manual describes in detail a variety of protocols for determining the properties and identity of a virus and its behavior in infected plants. A Springer Lab Manual.

Viruses require a special approach to establish their presence in a diseased plant since they are not visible, even under a light microscope. This manual describes in detail a variety of protocols for determining the properties and identity of a virus and its behavior in infected plants. A Springer Lab Manual.

Written for advanced undergraduate students, this book is a practical, in-depth guide to plant virology. Beginning with an introduction to viruses and their classification, the text describes virus pathology, including how viruses enter and move through plant cells and induce disease. Subsequent chapters discuss how viruses spread in the field and how to measure this. Throughout, the book remains reader-friendly, using focus boxes for clear, easy to obtain information, enabling students to quickly access relevant information but supply sufficient detail for advanced studies. In addition to basic information on virus biology there is an additional focus on applied virology, ideal for students undertaking agricultural studies for whom study of disease and its control is essential.

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Applied Plant Virology: Advances, Detection, and Antiviral Strategies provides an overview on recent developments and applications in the field of plant virology. The book begins with an introduction to important advances in plant virology, but then covers topics including techniques for assay detection and the diagnosis of plant viruses, the purification, isolation and characterization of plant viruses, the architecture of plant viruses, the replication of plant viruses, the physiology of virus-infected hosts, vectors of plant viruses, and the nomenclature and classification of plants. The book also discusses defense strategies by utilizing antiviral agents and management strategies of virus and viroid diseases. With contributions from an international collection of experts, this book presents a practical resource for plant virologists, plant pathologists, horticulturalists, agronomists, biotechnologists, academics and researchers interested in up-to-date technologies and information that advance the field of plant virology. Covers the detection, control and management of plant viruses Discusses antiviral strategies, along with mechanisms of systemic induced resistance to enhance the defense of plants against viruses Provides contributory chapters from expert plant virologists from different parts of the world

For the past twenty years I have worked as an applied plant virologist, attempting to identify and control virus diseases in field crops. During the last ten years it has been my privilege to present short courses in plant virology to final-year students studying plant pathology, micro biology and general botany. Throughout the period I have been lecturing, it has been possible to recommend several excellent 'library' books for further reading in plant virology, but there has been no publication covering applied plant virology that a student might consider purchasing. With teaching requirements in mind this book has been written to provide a concise introduction to applied plant virology based on the experiences I have gained working on virus diseases, both in an applied laboratory and in the field. The text concentrates on introducing the reader to aspects of plant virology that would be encountered every day by an applied virologist trying to identify viruses and develop control measures for virus diseases of crop plants. Although a brief introduction to virus structure and its terminology is given in the opening chapter of the book, no attempt is made to cover in detail the more fundamental aspects of virus structure, biochemistry and replication. Similarly, the symptoms caused by individual viruses are not described, although the various types of symptoms that plant viruses cause and which might be encountered by a student or research worker are described.

This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.