Principles Of Bioseparations Engineering

Bioseparations engineering deals with the scientific and engineering principles involved in large-scale separation and purification of biological products. It is a key component of most chemical engineering/biotechnology/bioprocess engineering programmes. This book discusses the underlying principles of bioseparations engineering written from the perspective of an undergraduate course. It covers membrane based bioseparations in much more detail than some of the other books on bioseparations engineering. Based largely on the lecture notes the author developed to teach the course, this book is especially suitable for use as an undergraduate level textbook, as most other textbooks are targeted at graduate students.

Multidisciplinary resource for graduate studies and the biotechnology industry Knowledge of the genetic basis of biological functioning continues to grow at an astronomical rate, as do the challenges and opportunities of applying this information to the production of therapeutic compounds, specialty biochemicals, functional food ingredients, environmentally friendly biocatalysts, and new bioproducts from renewable resources. While genetic engineering of living organisms transforms the science of genomics into treatments for cancer, diabetes, and heart disease, or products for industry and agriculture, the science and technology of bioseparations are the keys to delivering these products in a purified form suitable for use by people. The methods, theory, and materials that reduce the science of bioseparations to practice, whether in the laboratory or the plant, are the subjects of Bioseparations Engineering. Examples address purification of biomolecules ranging from recombinant proteins to gene therapy products, with footnotes detailing economics of the products. Mechanistic analysis and engineering design methods are given for: * Isocratic and gradient chromatography * Sedimentation, centrifugation, and filtration * Membrane systems * Precipitation and crystallization Topics addressed within this framework are: stationary phase selection; separations development; modeling of ion exchange, size exclusion, reversed phase, hydrophobic interaction, and affinity chromatography; the impact of regulatory issues on chromatography process design; organization of separation strategies into logical sequences of purification steps; and bridges between molecular biology, combinatorial methods, and separations science. A result of teaching and developing the subject matter over ten years, Bioseparations Engineering is an ideal text for graduate students, as well as a timely desk book for process engineers, process scientists, researchers, and research associates in the pharmaceutical, food, and life sciences industries.

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field of bioseparations. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. New to this second edition are updated discussions of moment analysis, computer simulation, membrane chromatography, and evaporation, among others, as well as revised problem sets. Unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations, and is the premier text in the field.

This systematically organized and well-balanced book compresses within the covers of a single volume the theoretical principles and techniques involved in bio-separations, also called downstream processing. These techniques are derived from a range of subjects, for example, physical chemistry, analytical chemistry, bio-chemistry, biological science and chemical engineering. Organized in its 15 chapters, the text covers in the first few chapters topics related to chemical engineering unit operations such as filtration, centrifugation, adsorption, extraction and membrane separation as applied to bioseparations. The use of chromatography as practiced at laboratory as well as industrial scale operation and related techniques such as gel filtration, affinity and pseudoaffinity chromatography, ion-exchange chromatography, electrophoresis and related methods have been discussed. The important applications of these techniques have also been highlighted.

Industrial Bioseparations offers comprehensive coverage of bioseparations including all unit operations. This new book offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today. It is laid out in a methodical way with preliminary chapters covering general approaches to bioseparations for commercially important biomacromolecules, thermodynamics and mass transfer principles, and following chapters addressing unit operations such as filtration and chromatography. Lab experiments are included which emphasize obtaining scale up parameters as well as commonly used operating conditions are included.

Separation Process Principles with Applications Using Process Simulator, 4th Edition is the most comprehensive and up-to-date treatment of the major separation operations in the chemical industry. The 4th edition focuses on using process simulators to design separation processes and prepares readers for professional practice. Completely rewritten to enhance clarity, this fourth edition provides engineers with a strong understanding of the field. With the help of an additional co-author, the text presents new information on bioseparations throughout the chapters. A new chapter on mechanical separations covers settling, filtration and centrifugation including mechanical separations in biotechnology and cell lysis. Boxes help highlight fundamental equations. Numerous new examples and exercises are integrated throughout as well.

The bioseparation engineering of today includes downstream process engineering such as waste water, material and gas treatment. Taking this tendency into account, bioseparation engineers gathered in Japan as a special research group under the main theme of "Recovery and Recycle of Resources to Protect the Global Environment". The scope of this book is based on the conference, and deals not only with recent advances in bioseparation engineering in a narrow sence, but also the environmental engineering which includes waste water treatment and bioremediation. The contributors of this book cover many disciplines such as chemical engineering, analytical chemistry, biochemistry, and microbiology. Bioseparation Engineering will stimulate young engineers and scientists who will develop bioseparation engineering further in the 21st century, and contribute to a world-wide attention to the global environment