Proteomics And Systems Biology

Proteomics and Systems Biology, Volume 127 in the Advances in Protein Chemistry and Structural Biology series, outlines current proteomic methodologies and discuss the challenges in future applications of systems biology in a number of biomedical/bioscience subjects. In last few decades, advances in genomics, proteomics, metabolomics, glycomics, venomics, etc., have produced vast large-scale datasets that need to be analyzed with a single main objective of understanding biological systems as a whole. Such understanding will allow us to predict and characterize the dynamic properties of biological systems. Integrates experimental and computational methods for understanding biological systems as a whole Contains timely chapters written by well-renowned authorities in their field Includes well supported content that is accompanied by a number of high-quality illustrations, figures and tables, hence it targets a wide audience of specialists, researchers and students

This detailed book highlights the diverse techniques and applications of proteomics in an accessible, informative, and concise manner. The collection features sample preparation from distinct extraction, quantification, enrichment, modification, as well as interactome methodology for the in-depth exploration of biological systems, and the application of proteomics to clinical, infectious disease, and agricultural practices. Moreover, cutting-edge bioinformatics tools, encompassing machine learning and data integration strategies, are explored, as are techniques expanding beyond proteomics into the realm of metabolomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls. Comprehensive and practical, Proteomics in Systems Biology: Methods and Protocols emphasizes the importance of proteomics and demonstrates a plethora of approaches for investigating diverse biological entities from a systems perspective.

This book constitutes the thoroughly refereed post-proceedings of two joint RECOMB 2006 satellite events: the Second Annual Workshop on Systems Biology, RSB 2006, and the First Biennial Workshop on Computational Proteomics, RCP 2006, held in San Diego, CA, USA in December 2006. The papers cover various aspects of systems biology and explore the use of computational mass spectrometry in various proteomic applications.

This book explains omics at the most basic level, including how this new concept can be properly utilized in molecular and systems biology research. Most reviews and books on this topic have mainly focused on the technicalities and complexity of each omics’ platform, impeding readers to wholly understand its fundamentals and applications. This book tackles such gap and will be most beneficial to novice in this area, university students and even researchers. Basic workflow and practical guidance in each omics are also described, such that scientists can properly design their experimentation effectively. Furthermore, how each omics platform has been conducted in our institute (INBIOSIS) is also detailed, a comprehensive example on this topic to further enhance readers’ understanding. The contributors of each chapter have utilized the platforms in various manner within their own research and beyond. The contributors have also been interactively integrated and combined these different omics approaches in their research, being able to systematically write each chapter with the conscious knowledge of other inter-relating topics of omics. The potential readers and audience of this book can come from undergraduate and postgraduate students who wish to extend their comprehension in the topics of molecular biology and big data analysis using omics platforms. Furthermore, researchers and scientists whom may have expertise in basic molecular biology can extend their experimentation using the omics technologies and workflow outlined in this book, benefiting their research in the long run.

Proteomics, like other post-genomics tools, has been growing at a rapid pace and has important applications in numerous fields of science. While its use in animal and veterinary sciences is still limited, there have been considerable advances in this field in recent years, in areas as diverse as physiology, nutrition and food of animal origin processing. This is mainly as a consequence of a wider availability and better understanding of proteomics methodologies by animal and veterinary researchers. This book provides a comprehensive, state-of-the-art account of the status of farm-animal proteomics research, focusing on the principles behind proteomics methodologies and its specific applications and offering clear example.

This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.

This book constitutes the thoroughly refereed post-proceedings of two joint RECOMB 2006 satellite events: the Second Annual Workshop on Systems Biology, RSB 2006, and the First Biennial Workshop on Computational Proteomics, RCP 2006, held in San Diego, CA, USA in December 2006. The papers cover various aspects of systems biology and explore the use of computational mass spectrometry in various proteomic applications.

Confidently face the challenges of proteomics research specific to plant science with the information in Plant Proteomics, which will introduce you to the techniques and methodologies required for the study of representative plant species. Read about proteomics studies in Arabidopsis, rice, and legumes and find information about common technologies like mass spectrometry and gel electrophoresis. Discover expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology, understand how to conduct proteomics studies in developing countries and underfunded laboratories, and gain access to guidelines for sample preparation.