Separate Lifetimes

Now in its fourth printing, this book contains Irving Townsend's classic animal essays first published in Harper's Magazine, Country Journal and Reader's Digest. Separate Lifetimes has continued to surprise and delight animal lovers since 1986.

During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Excited State Lifetime Measurements attempts to assist in clarifying and unifying the many characteristics and definitions of excited state lifetime measurements. The contents of this book are derived from a series of lectures presented to a research group in the University of New Mexico in 1967. The relevance as well as the methods and measurements of data treatment of excited state lifetimes are featured in this book. The first three chapters provide a brief discussion on concepts and applications of excited state lifetime measurements. Experimental methods and systems are also introduced in these chapters. Chapter 4 delves into more complex systems (serial decay kinetics and resonance energy transfer) while Chapter 5 focuses on the method of least squares fitting, its uses, and misuses. Chapters 6 to 8 mainly discuss the convolution integral and its different applications while Chapter 9 gives a more detailed presentation of instrumentation. The last two chapters discuss special errors and approaches to new methodologies regarding the study of the excited state lifetime measurements. The book will be useful to students and scientists including analytical chemists, photochemists, photobiologists, spectroscopists, and physicists.

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

In this major work, Jeffrey Hopkins, on e of the world's foremost scholar-practitioners of Tibetan Buddhism, offers a clear exposition of the Prasangika-Madhyamaka view of emptiness as presented in the Ge-luk-ba tradition of Tibetan Buddhism. In bringing this remarkable and complex philosophy to life, he describes the meditational practices by which emptiness can be realized and shows throughout that, far from being merely abstract, these teachings can be vivid and utterly practical. Presented in six parts, this book is indispensable for those wishing to delve deeply into Buddhist thought.

Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Self-Trapped Excitons discusses the structure and evolution of the self-trapped exciton (STE) in a wide range of materials. It includes a comprehensive review of experiments and extensive tables of data. Emphasis is given throughout to the unity of the basic physics underlying various manifestations of self-trapping, with the theory being developed from a localized, atomistic perspective. The topics treated in detail in relation to STE relaxation include spontaneous symmetry breaking, lattice defect formation, radiation damage, and electronic sputtering.