Singlet Oxygen Detection And Imaging

Singlet Oxygen, the lowest electronically excited state of molecular oxygen, is highly reactive and involved in many chemical and biological processes. It is one major mediator during photosensitization, which has been used by mankind since ancient times, even though the mechanisms behind it were understood only about half a century ago. The combination of high reactivity and very long natural lifetime allows for direct optical detection of singlet oxygen and its interactions using its characteristic phosphorescence at around 1270 nm. Since this emission is very weak, optical detection was technically very challenging for a long time. Therefore, even today, most laboratories only exploit the high reactivity to observe the interaction with sensor molecules, rather than singlet oxygen emission itself. However, in recent years highly sensitive optical detection was developed, the authors being major contributors. This book is dedicated to the detection of singlet oxygen, discussing possibilities, pitfalls and limits of the various methods with a special focus on time-resolved phosphorescence and the kinetics of singlet oxygen generation and decay including involved and related processes, discussing investigated systems with various complexity from solutions over in vitro to in vivo. The long-standing paradigm that singlet oxygen phosphorescence is a benchmark for detection systems rather than an option for process observation is still ubiquitous and this book hopes to contribute in overcoming this still prevailing bias.

Meeting the desire for a comprehensive book that collects and curates the vast amount of knowledge gained in the field of singlet oxygen, this title covers the physical, chemical and biological properties of this reactive oxygen species and also its increasingly important applications across chemical, environmental and biomedical areas.The editors have a long and distinguished background in the field of singlet oxygen chemistry and biomedical applications, giving them a unique insight and ensuring the contributions attain the highest scientific level.The book provides an up to date reference resource for both the beginner and experienced researcher and crucially for those working across disciplines such as photochemistry, photobiology and photomedicine.

Reactive Oxygen Species—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Superoxides. The editors have built Reactive Oxygen Species—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Superoxides in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Reactive Oxygen Species—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Meeting the desire for a comprehensive book that collects and curates the vast amount of knowledge gained in the field of singlet oxygen, this title covers the physical, chemical and biological properties of this reactive oxygen species and also its increasingly important applications across chemical, environmental and biomedical areas.The editors have a long and distinguished background in the field of singlet oxygen chemistry and biomedical applications, giving them a unique insight and ensuring the contributions attain the highest scientific level.The book provides an up to date reference resource for both the beginner and experienced researcher and crucially for those working across disciplines such as photochemistry, photobiology and photomedicine.

The application of imaging techniques in plant and agricultural sciences had previously been confined to images obtained through remote sensing techniques. Technological advancements now allow image analysis for the nondestructive and objective evaluation of biological objects. This has opened a new window in the field of plant science. Plant Image Analysis: Fundamentals and Applications introduces the basic concepts of image analysis and discusses various techniques in plant imaging, their applications, and future potential. Several types of imaging techniques are discussed including RGB, hyperspectral, thermal, PRI, chlorophyll fluorescence, ROS, and chromosome imaging. The book also covers the use of these techniques in assessing plant growth, early detection of disease and stress, fruit crop yield, plant chromosome analysis, plant phenotyping, and nutrient status both in vivo and in vitro. The book is an authoritative guide for researchers and those teaching in the fields of stress physiology, precision agriculture, agricultural biotechnology, and cell and developmental biology. Graduate students and professionals using machine vision in plant science will also benefit from this comprehensive resource.

Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.