Spectral Techniques In Proteomics
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Spectral Techniques In Proteomics
Author | : Daniel S. Sem |
Publsiher | : CRC Press |
Total Pages | : 464 |
Release | : 2007-03-30 |
Genre | : Medical |
ISBN | : 9781420017090 |
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Facilitating the innovation, development, and application of new spectroscopic methods in proteomics, Spectral Techniques in Proteomics provides a broad overview of the spectroscopic toolbox that can be used, either with proteome or sub-proteome mixtures or with individual/purified proteins studied in parallel. It gives a modest overview of
Neuroproteomics
Author | : Oscar Alzate |
Publsiher | : CRC Press |
Total Pages | : 356 |
Release | : 2009-10-26 |
Genre | : Medical |
ISBN | : 9781420076264 |
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In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
Modern Proteomics Sample Preparation Analysis and Practical Applications
Author | : Hamid Mirzaei,Martin Carrasco |
Publsiher | : Springer |
Total Pages | : 533 |
Release | : 2016-12-14 |
Genre | : Science |
ISBN | : 9783319414485 |
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This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.
Computational Methods for Mass Spectrometry Proteomics
Author | : Ingvar Eidhammer,Kristian Flikka,Lennart Martens,Svein-Ole Mikalsen |
Publsiher | : John Wiley & Sons |
Total Pages | : 296 |
Release | : 2008-02-28 |
Genre | : Medical |
ISBN | : 0470724293 |
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Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data. Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.
Mass Spectrometry Data Analysis in Proteomics
Author | : Rune Matthiesen |
Publsiher | : Springer Science & Business Media |
Total Pages | : 322 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 9781597452755 |
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This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. The volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.
New and Emerging Proteomic Techniques
Author | : Dobrin Nedelkov,Randall W. Nelson |
Publsiher | : Springer Science & Business Media |
Total Pages | : 237 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 9781597450263 |
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Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Introduction to Proteomics
Author | : Daniel Liebler |
Publsiher | : Springer Science & Business Media |
Total Pages | : 210 |
Release | : 2001-12-04 |
Genre | : Medical |
ISBN | : 9780896039919 |
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Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.
Proteome Characterization and Proteomics
Author | : Timothy D. Veenstra,Richard D. Smith |
Publsiher | : Academic Press |
Total Pages | : 445 |
Release | : 2003-10-01 |
Genre | : Science |
ISBN | : 9780080569154 |
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The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies. Discusses proteomics in the postgenomic age Includes various strategies for quantitative proteomics Covers the role of MS in structural functional proteomics and proteomics in drug discovery and bioinformatics