Surface Plasmon Resonance

Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.

This is a comprehensive treatment of the field of SPR sensors, in three parts. Part I introduces principles of surface plasmon resonance bio-sensors, electromagnetic theory of surface plasmons, theory of SPR sensors and molecular interactions at sensor surfaces. Part II examines the development of SPR sensor instrumentation and functionalization methods. Part III reviews applications of SPR biosensors in the study of molecules, and in environmental monitoring, food safety and medical diagnostics.

In this book, experts in the field present a wide variety of applications involving commercially available SPR instruments. The book fills a need for well-described, hands-on SPR experimental protocols, and it highlights the backgrounds of vital issues.

This book introduces the fundamentals and applications of the localized surface plasmon resonance (LSPR) property of noble metallic nanoparticles, with an emphasis on the biosensing applications of plasmonic nanoparticles, especially in living cell imaging and photothermal therapy. It provides an overview of the different operating principles of plasmonic sensors, particularly the single-nanoparticle-based detections, and a series of creative biosensors based on the modulation of different parameters of nanoparticles (particle size, shape, composition and surrounding medium) for label-free detection. The interparticle coupling effect, plasmon resonance energy transfer, electron transfer on plasmonics surface are also covered in this book. This book is intended for graduate students and researchers working in the interdisciplinary field combining chemistry, biology, material science and nanophotonics. Yi-Tao Long is a Professor at the School of Chemistry and Molecular Engineering, East China University of Science and Technology, China.

2D Materials for Surface Plasmon Resonance-based Sensors offers comprehensive coverage of recent design and development (including processing and fabrication) of 2D materials in the context of plasmonic-based devices. It provides a thorough overview of the basic principles and techniques used in the analysis and design of 2D material-based optical sensor systems. Beginning with the basic concepts of plasmon/plasmonic sensors and mathematical modelling, the authors explain the fundamental properties of 2D materials, including Black Phosphorus (BP), Phosphorene, Graphene, Transition metal dichalcogenides (TMDCs), MXene's and SW-CNT. It also details the applications of these emerging materials in clinical diagnosis and their future trends. This text will be useful for practising engineers, undergraduate and postgraduate students. Key Features Presents the fundamental concepts of 2D material assisted fibre optic and prism based SPR sensor in a student-friendly manner. Includes the recent synthesis and characterization techniques of 2D materials. Provides computational results of recently discovered electronic and optical properties of the 2D materials along with their effectiveness in the field of plasmonic sensors. Presents emerging applications of novel 2D material-based plasmonic sensors in the field of chemical, bio-chemical and biosensing.

SPR is real-time, label-free measurements of binding kinetics and affinity. This has distinct advantage over radioactive or fluorescent labelling methods, in terms of: 1) ligand-analyte binding kinetics, that can be probed without the costly and time-consuming labeling process that may interfere with molecular binding interactions; 2) binding rates and affinity can be measured directly and 3) low affinity interactions in high protein concentrations for can be characterized with less reagent consumption than other equilibrium measurement techniques; 4) Label-free detection of molecular interactions presents an attractive alternative. This is particularly true for protein targets where labels can compromise protein function. Label-free methods are also desirable because they are typically compatible with real-time detection, enabling the determination of rates of association. The success of SPR biosensors was indicated by the growing number of commercially available instruments. Since Biacore AB (originally Pharmacia Biosensor AB) launched the first commercial SPR biosensor on market in 1990, there have been many more competing SPR instruments including IASys (Affinity Sensors), SPR-670 (Nippon Laser Electronics), IBIS (IBIS Technology BV), TISPR (Texas Instruments), etc.

Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events and with the biosensor field expanding more applications are being found. In response to the market, an update to the original title which was published in 2008 is now appropriate. With over fifty percent of the material being updated, this book provides a total system description including optics, fluidics and sensor surfaces. Spanning theory, instrumentation and applications, it covers all the relevant issues for the practicing researcher. Unlocking the potential for SPR by showing highly exciting and unique opportunities for unraveling the functional relationships of complex biological processes, it is intended for a wide audience. A comprehensive and accessible source it contains expanded tutorial details to inspire students and guide them in this technology.

Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations of each technique are presented. Case studies illustrate the application of each technique in real applied examples. Finally, the book covers recent developments in areas such as electron microscopy with discussions of their possible impact on future drug discovery. This is a go-to volume for biophysicists, analytical chemists and medicinal chemists providing a broad overview of techniques of contemporary interest in drug discovery.