Theory And Application Of Microbiological Assay

Theory and Application of Microbiological Assay first presents an overview of microbiological assay, including general principles, basic techniques, capabilities, and limitations. This book also explains when to use a certain microbiological assay for specific analysis, citing examples to further clarify the matter. Some chapters discuss the test organisms and solutions, wherein test solutions are the final dilutions at a series of two or more concentrations that have been prepared from reference standard and unknown for application to the test system. Other chapters explore the various aspects of agar diffusion assays, tube assays, and diffusion assays. The book further looks into the quality control procedures, assay design and evaluation, and assay replication. This book will serve as an introduction to newcomers to the field and as a reference source for established workers in microbiological assay.

A user-friendly guide for the evaluation of microbiological assays, this book provides a lucid explanation of the sources of error in microbiological assay and helps analysts choose efficient assay designs that will minimize those sources of error. The author discusses microbiological assay as a branch of pharmaceutical analysis and distinguishes it from biological assay in general. He draws attention to the microbiological aspects that may not be so obvious to the chemical analyst and to the analytical aspects that may not be so obvious to the microbiologist. The book expands on the guidance given in pharmacopoeias and helps readers choose the assay design most appropriate for the purpose of their assay.

Analytical Microbiology focuses on the processes, methodologies, developments, and approaches involved in analytical microbiology, including microbiological, antibiotic, and amino acid assays and dilution methods. The selection first offers information on the theory of antibiotic inhibition zones, microbiological assay using large plate methods, and dilution methods of antibiotic assays. Discussions focus on serial dilution assay, requirements for accurate assay, microbiological assay of riboflavin, laws of adsorption and partition, mechanisms of antibiotic action, and biological considerations affecting the use of statistical methods. The text then ponders on the elements of photometric assaying and automation of microbiological assays. The manuscript elaborates on antibiotic substances, vitamins, and amino acids. Topics include assay organisms, validity, specificity, reliability, and calculation of results of amino acid assays, bacitracin, chloramphenicol, dihydrostreptomycin, erythromycin, neomycin, and streptomycin. The selection is a dependable reference for researchers interested in analytical microbiology.

Semi-automated method for microbiological vitamin and vitamin and antibiotic assays; An automatic dilution apparatus for use in vitamin and antibiotic assay; Application of automated assay of asparaginase and other ammonia-releasing enzymes to the identification of bacteria; An automatic plating-out machine for microbiological assay employing an 8 X 8 design; Virus concentration by means of soluble ultrafilters; The assay of thermally induced inhibitors derived from nitrite; The assay of nisin in foods; Serological methods for the assay of staphylococcal enterotoxins; Assay of myo-inositol using the yeast Kloeckera apiculata (K. brevis); Notes on the preservation and checking of vitamin assay bacteria; A simple, rapid assay for the measurement of antibiotic concentrations in human serum; Bio-assay of agricultural and horticultural fungicides; Testing biocidal paints; Microbiological assay of chemicals for the protection of wood; Techniques for the assay of effects of herbicides on the soil microflora; The effect of herbicides on cellulose decomposition; Use of micro-algae for the assay of herbicides.

Designed for major and non-major students taking an introductory level microbiology lab course. Whether your course caters to pre-health professional students, microbiology majors or pre-med students, everything they need for a thorough introduction to the subject of microbiology is right here.

This newest addition to the best-selling Microbiology: Laboratory Theory & Application series of manuals provides an excellent value for courses where lab time is at a premium or for smaller enrollment courses where customization is not an option. The Essentials edition is intended for courses populated by nonmajors and allied health students and includes exercises selected to reflect core microbiology laboratory concepts.

Analytical chemists in industry are frequently faced with situations where a basic understanding of microbiology would be an advantage, for instance in the analysis of bacteria in food. Microbiology for the Analytical Chemist has been written specifically for analytical chemists who have little or no knowledge of microbiology, but might be required to interpret microbiological results. This book covers a wide range of microbiological situations in analysis. It deals with the question of establishing when a sample is contaminated, the problems of counting and identifying micro-organisms and establishing what effect they will have on the sample. The book examines the microbial contents of water and food. It also looks at the procedures for disinfecting and preservative testing. Traditional laboratory methods are discussed, and new rapid techniques are also considered. Microbiology for the Analytical Chemist is unusual in that it pulls together those aspects of microbiology which are of interest to analytical chemists and explains them at a basic level using practical situations as examples. This book will also be of interest to analytical chemists in academic or industrial laboratories, where there is no fund of microbiological experience to draw on.

Completely revised and updated Pharmaceutical Microbiologycontinues to provide the essential resource for the 21st centurypharmaceutical microbiologist "....a valuable resource for junior pharmacists graspingan appreciation of microbiology, microbiologists entering thepharmaceutical field, and undergraduate pharmacy students." Journal of Antimicrobial Chemotherapy ".....highly readable. The content is comprehensive, withwell-produced tables, diagrams and photographs, and is accessiblethrough the extensive index." Journal of Medical Microbiology WHY BUY THIS BOOK? Completely revised and updated to reflect the rapid pace ofchange in the teaching and practice of pharmaceuticalmicrobiology Expanded coverage of modern biotechnology, including genomicsand recombinant DNA technology Updated information on newer antimicrobial agents and theirmode of action Highly illustrated with structural formulas of organiccompounds and flow diagrams of biochemical processes