Tree Transgenesis

Tree improvement is necessary to meet the growing demand for renewable wood resource and the time is ripe for a critical evaluation of the chances and challenges of tree transgenesis. This book provides an up-to-date review of the present state of genetic engineering of trees. Biosafety and risk assessment are treated in detail, and future experimental tasks are discussed. The book provides a sound basis for decision-making processes in politics.

Annotation. "This volume on Transgenic Trees, comprising 22 chapters, deals with the genetic transformation of fruit and forest trees." "It is of special interest to advanced students, teachers and research workers in the field of forestry, horticulture, molecular biology, plant tissue culture, botany, and plant biotechnology in general."--BOOK JACKET. Title Summary field provided by Blackwell North America, Inc. All Rights Reserved.

Forest trees cover 30% of the earth's land surface, providing renewable fuel, wood, timber, shelter, fruits, leaves, bark, roots, and are source of medicinal products in addition to benefits such as carbon sequestration, water shed protection, and habitat for 1/3 of terrestrial species. However, the genetic analysis and breeding of trees has lagged behind that of crop plants. Therefore, systematic conservation, sustainable improvement and pragmatic utilization of trees are global priorities. This book provides comprehensive and up to date information about tree characterization, biological understanding, and improvement through biotechnological and molecular tools.

This book provides up-to-date information on the environmental impact of transgenic trees on genetically modified tree (GMT) communication strategy. It is useful to public/private organisations as well as to private and public research bodies and universities worldwide since it reports on the global status of GMT research and policy. A high number of genetically modified trees (GMTs) with altered or novel characteristics have been produced in the last 15 years. However, their very low public acceptance is a basic problem in their commercialization. Breeders anticipate economic and ecological benefits, like reduced product costs and less pressure on native forests, while opponents fear risks, such as unintended spread of GMTs. But what is true? To answer this question, the COST Action FP0905 focused on key aspects related to GMTs: (a) biological characterization; (b) assessment of possible environmental impacts; (c) socio-economic implications and public acceptance/concerns; (d) providing science-based information to communicate with the public.

The book is written for policy experts, life scientists, government and business leaders, biotechnology writers and social activists. Few decision-makers realize the unprecedented degree to which transgenic technology is now possible for forests on a commercial scale. Only a handful of the 550 living conifer species is used for commodity value and even fewer species are being developed for transgenic plantations. Transgenic field trials started within the last decade but no transgenic pine plantations exist in 2005. But emergence of transgenic forest trees is still so recent that dialogue about the pros and cons is confined to the scientific community. And dialogue must move out into the public domain. The goal of this volume is to provide content for public deliberations about the genetic composition of future forests. Its Section I is composed of provocative and opposing views on the question of transgenic conifer plantations. Sections II and III follow with research advances on relevant conifer genomics and ecology research, respectively. Section IV forecasts rates of technology adoption for different case studies. Finally, Section V compares the status of regulatory oversight of transgenic forest trees between Canada and the United States.

The introduction of novel genes into plants by genetic transformation holds great promise for plant breeding, and many crop species have been rendered virus-resistant by expression of viral sequences. However, it is essential to also evaluate the potential risks associated with this new technology. Among the types of genetically modified plants that could represent potential ecological risks, ones expressing viral sequences pose questions of particular interest. In this volume special attention is given to recombination in plants expressing sequences of RNA or DNA viruses, heterologous encapsidation or other forms of complementation in plants expressing coat protein genes, potential deleterious effects of satellite RNAs associated with cucumber mosaic virus, and sexual transmission of virus resistance genes to potentially weedy relatives.

Since the first transgenic plants were produced back in the early 1980s, there have been substantial developments towards the genetic engineering of most crops of our world. Initial studies using isolated plant cells and removing their cell walls to form protoplasts, offered the possibility of transferring genetic material by Agrobacterium-mediated gene transfer, chemical agents or electrical charges. However, in those cases were isolated protoplasts could be transformed, often, a shoot regeneration system was not available to induce the production of transgenic plants and any such regenerated plants were subject to mutation or chromosomal of cultured plant organs, such as leaf abnormalities. By the mid-1980s, the use disks, offered the convenience of combining gene transfer, plant regeneration and selection of transformants in a single system. This approach, enabled the production of stable, phenotypically-normal, transgenic potato and tomato plants in culture. By the late 1980s, the use of biolistics offered a means of inserting foreign genes into plant cells which where inaccessible to Agrobacterium infection. Even today, this technology is now standard practice for the production of some transgenic plants.

Forest trees cover 30% of the earth's land surface, providing renewable fuel, wood, timber, shelter, fruits, leaves, bark, roots, and are source of medicinal products in addition to benefits such as carbon sequestration, water shed protection, and habitat for 1/3 of terrestrial species. However, the genetic analysis and breeding of trees has lagged