Who Laboratory Manual For The Examination And Processing Of Human Semen

Semen analysis may be useful in both clinical and research settings, for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility regulation and other interventions. This manual provides updated, standardized, evidence-based procedures and recommendations for laboratory managers, scientists and technicians to follow in examining human semen in a clinical or research setting. Detailed protocols for routine, optional and research tests are elaborated. The fifth edition includes new information on sperm preparation for clinical use or specialized assays and on cryopreservation, an expanded section on quality control in the semen analysis laboratory and evidence-based reference ranges and reference limits for various semen characteristics. The methods described are intended to improve the quality of semen analysis and the comparability of results from different laboratories.

The definitive and essential source of reference for all laboratories involved in the analysis of human semen.

"Semen analysis may be useful in both clinical and research settings, for investigating male fertility status as well as monitoring spermatogenesis during and following male fertility regulation and other interventions. This manual provides updated, standardized, evidence-based procedures and recommendations for laboratory managers, scientists and technicians to follow in examining human semen in a clinical or research setting. Detailed protocols for routine, optional and research tests are elaborated. The fifth edition includes new information on sperm preparation for clinical use or specialized assays and on cyropreservation, an expanded section on quality control in the semen analysis laboratory and evidence-based reference ranges and reference limits for various semen characteristics. The methods described are intended to improve the quality of semen analysis and the comparability of results from different laboratories." --Publisher.

Infertility is an important public health problem affecting around 15% of couples worldwide. A male factor contributes to about 50% of all cases of infertility. Male infertility could be related to a variety of congenital or acquired causes that lead to abnormal reproductive potential and poor sperm quality and function. To this date, semen analysis remains the cornerstone of male infertility evaluation. Clinicians widely reference the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen, which provides a detailed description of a variety of semen tests. However, the manual does not provide a sufficient clinical context for the various tests that are described. This book is intended to bridge the gap between the laboratory manual and the clinician by explaining in detail the test indication, its clinical utility, interpretation of results, and presenting a decision algorithm based on the test results. Thebook has 19 chapters grouped under seven sections that follow the chapters of the sixth edition of the WHO manual. Each chapter is written and reviewed by a team of senior clinicians, thus bringing a perspective that is truly clinically relevant. This book will be of great value to all clinicians involved in the management of male infertility and will also be of interest to laboratory scientists involved in semen testing by providing them with clinical perspectives of the test they perform. Human Semen Analysis: From the WHO Manual to the Clinical Management of Infertile Men is a useful guide for utilizing the WHO manual, interpreting test results, and deciding what subsequent clinical actions to take.

These proceedings of the 2018 XIII International Symposium on Spermatology focus on comparative biology, and encourages discussion and the exchange of ideas. The aim of this Symposium was to provide a unique opportunity and bring together scientists from a wide spectrum of research fields – human, domestic animals and other mammals, vertebrates, insects, and plants. The underlying focus is on the function of the spermatozoon – a common feature for sexual reproduction, but extremely varied. By exploring the variability, a better understanding of male reproductive functions can develop. These proceedings address the mechanisms of physiology and pathophysiology, rather than diagnosis and treatment. The symposium featured keynote lectures by invited speakers, followed by presentations on specific aspects of the general topic of the session. Experimental studies are given priority over clinical studies of patient populations. The proceedings comprise both keynote speakers' texts and selected free communications. Posters were considered for publication in the proceedings, and the volume includes exhibited materials on the work of prominent spermatologists, highlighting their important past achievements in the field.

This state-of-the-art laboratory manual includes 20 clinical protocols used daily for the investigation of the infertile male, presented with easy to understand, step-by-step methodology. The protocols are arranged from routine to advanced laboratory procedures common to clinical practice, including computer-assisted semen analysis, sperm preparation for IUI by density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation test by TUNEL method, among others. The methodology in each protocol follows best practice guidelines made clearer by professionally hand-drawn illustrations covering most of the important steps and equipment. The authors, hailing from the world-renowned Andrology Center at Cleveland Clinic, have over 50 years of combined first-hand experience in managing very busy diagnostic and research facilities in male infertility and andrology. The book will be an indispensable resource for thousands of laboratory technologists, clinicians and reproductive professionals (andrologists, embryologist, etc.) engaged in the diagnosis and management of infertile men around the world.

Focusing on modern sperm function testing, this guide is essential in selecting sperm that will produce viable and healthy embryos.

Preceded by A practical guide to basic labratory andrology / Lars Bjèorndahl... [et al.]. 2010.