Accessing Uncultivated Microorganisms

Providing a comprehensive overview and discussing developments in the field, this book details various innovative methods used in microbial ecology and environmental microbiology. It also includes all aspects of microbial diversity from bacteria and fungi to protists.

In 1898, an Austrian microbiologist Heinrich Winterberg made a curious observation: the number of microbial cells in his samples did not match the number of colonies formed on nutrient media (Winterberg 1898). About a decade later, J. Amann qu- tified this mismatch, which turned out to be surprisingly large, with non-growing cells outnumbering the cultivable ones almost 150 times (Amann 1911). These papers signify some of the earliest steps towards the discovery of an important phenomenon known today as the Great Plate Count Anomaly (Staley and Konopka 1985). Note how early in the history of microbiology these steps were taken. Detecting the Anomaly almost certainly required the Plate. If so, then the period from 1881 to 1887, the years when Robert Koch and Petri introduced their key inventions (Koch 1881; Petri 1887), sets the earliest boundary for the discovery, which is remarkably close to the 1898 observations by H. Winterberg. Celebrating its 111th anniversary, the Great Plate Count Anomaly today is arguably the oldest unresolved microbiological phenomenon. In the years to follow, the Anomaly was repeatedly confirmed by all microb- logists who cared to compare the cell count in the inoculum to the colony count in the Petri dish (cf., Cholodny 1929; Butkevich 1932; Butkevich and Butkevich 1936). By mid-century, the remarkable difference between the two counts became a universally recognized phenomenon, acknowledged by several classics of the time (Waksman and Hotchkiss 1937; ZoBell 1946; Jannasch and Jones 1959).

Microbial Ecology of Wastewater Treatment Plants presents different methods and techniques used in microbial ecology to study the interactions and evolution of microbial populations in WWTPs, particularly the new molecular tools developed in the last decades. These molecular biology-based methods (e.g. studies of DNA, RNA and proteins) provide a high resolution of information compared to traditional ways of studying microbial wastewater populations, such as microscopic examination and culture-based methods. In addition, this book addresses the ability of microorganisms to degrade environmental pollutants. Describes application of different Omics tools in Wastewater treatment plants (WWTPs) Demonstrates the role of microorganisms in WWTPs Includes discussions on the microbial ecology of WWTPs Covers the microbial diversity of activated sludge Emphasizes cutting-edge molecular tools

The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Litera

Microbial communities and their functions play a crucial role in the management of ecological, environmental and agricultural health on the Earth. Microorganisms are the key identified players for plant growth promotion, plant immunization, disease suppression, induced resistance and tolerance against stresses as the indicative parameters of improved crop productivity and sustainable soil health. Beneficial belowground microbial interactions with the rhizosphere help plants mitigate drought and salinity stresses and alleviate water stresses under the unfavorable environmental conditions in the native soils. Microorganisms that are inhabitants of such environmental conditions have potential solutions for them. There are potential microbial communities that can degrade xenobiotic compounds, pesticides and toxic industrial chemicals and help remediate even heavy metals, and thus they find enormous applications in environmental remediation. Microbes have developed intrinsic metabolic capabilities with specific metabolic networks while inhabiting under specific conditions for many generations and, so play a crucial role. The book Microbial Interventions in Agriculture and Environment is an effort to compile and present a great volume of authentic, high-quality, socially-viable, practical and implementable research and technological work on microbial implications. The whole content of the volume covers protocols, methodologies, applications, interactions, role and impact of research and development aspects on microbial interventions and technological outcomes in prospects of agricultural and environmental domain including crop production, plan-soil health management, food & nutrition, nutrient recycling, land reclamation, clean water systems and agro-waste management, biodegradation & bioremediation, biomass to bioenergy, sanitation and rural livelihood security. The covered topics and sub-topics of the microbial domain have high implications for the targeted and wide readership of researchers, students, faculty and scientists working on these areas along with the agri-activists, policymakers, environmentalists, advisors etc. in the Government, industries and non-government level for reference and knowledge generation.

Plants create a dynamic micro-biosphere in the soil, around the roots, called as ‘rhizosphere’, which harbors diverse number of microorganisms for sustaining their growth and development. A soil with diverse and multi-traits microbial communities is considered healthy to enhance crop productivity. In the last decades, rhizosphere biology has gained attention due to unraveling of new mechanisms, processes and molecules in the rhizosphere that contributes towards the promotion of plant productivity. The rhizospheric microbes and associated processes are being utilized for harnessing potential of soils in effective and sustainable functioning in the agro-ecosystems. Broadly, the book discusses rhizospheric microbes and their role in modulating functions of soil and crop plant. Specifically, it highlights conventional and modern aspects of rhizosphere microbes such as – microbiome in the rhizosphere, microbes as an indicator and promoter of soil health, rhizosphere microbes as biofertilizer, biostimulator and biofortifyer, microbial signaling in the rhizosphere, recent tools in deciphering rhizobiome, and regulatory mechanisms for commercialization of biofertilizer, biopesticide and biostimulator. The book is useful for agriculture scientist, biotechnologist, plant pathologist, mycologist, and microbiologist, farming community, scientist of R&D organization, as well as teaching community, researcher and student and policy maker.

Encyclopedia of Microbiology, Fourth Edition, Five Volume Set gathers both basic and applied dimensions in this dynamic field that includes virtually all environments on Earth. This range attracts a growing number of cross-disciplinary studies, which the encyclopedia makes available to readers from diverse educational backgrounds. The new edition builds on the solid foundation established in earlier versions, adding new material that reflects recent advances in the field. New focus areas include `Animal and Plant Microbiomes’ and ‘Global Impact of Microbes`. The thematic organization of the work allows users to focus on specific areas, e.g., for didactical purposes, while also browsing for topics in different areas. Offers an up-to-date and authoritative resource that covers the entire field of microbiology, from basic principles, to applied technologies Provides an organic overview that is useful to academic teachers and scientists from different backgrounds Includes chapters that are enriched with figures and graphs, and that can be easily consulted in isolation to find fundamental definitions and concepts

Microbes in Land Use Change Management details the various roles of microbial resources in management of land uses and how the microbes can be used for the source of income due to their cultivation for the purpose of biomass and bioenergy production. Using various techniques, the disturbed and marginal lands may also be restored eco-friendly in present era to fulfil the feeding needs of mankind around the globe. Microbes in Land Use Change Management provides standard and up to date information towards the land use change management using various microbial technologies to enhance the productivity of agriculture. Needless to say that Microbes in Land Use Change Management also considers the areas including generation of alternative energy sources, restoration of degraded and marginal lands, mitigation of global warming gases and next generation -omics technique etc. Land use change affects environment conditions and soil microbial community. Microbial population and its species diversity have influence in maintaining ecosystem balance. The study of changes of microbial population provides an idea about the variation occurring in a specific area and possibilities of restoration. Meant for a multidisciplinary audience Microbes in Land Use Change Management shows the need of next-generation omics technologies to explore microbial diversity. Describes the role of microbes in generation of alternative source of energy Gives recent information related to various microbial technology and their diversified applications Provides thorough insight in the problems related to landscape dynamics, restoration of soil, reclamation of lands mitigation of global warming gases etc. eco-friendly way using versatility of microbes Includes microbial tools and technology in reclamation of degraded, disturbed and marginal lands, mitigation of global warming gases