Analytical Techniques In Biotechnology

Biotechnology is a rapidly progressing field of science and technology. It integrates principles and applications from diverse fields such as genomics, immunology, and many more. This book strives to equip the reader with thorough knowledge about various techniques of applied biotechnology, its applications in the areas of medicine, agriculture, environment, etc., along with details about the latest equipment and analytical tools which would help the reader gain an in-depth perspective about the subject. This book elucidates new techniques and their applications in a multidisciplinary approach and is apt for students of biotechnology and associated disciplines of study.

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - Protein sequence and composition analysis - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

The aim of this book is to present a range of analytical methods that can be used in formulation design and development and focus on how these systems can be applied to understand formulation components and the dosage form these build. To effectively design and exploit drug delivery systems, the underlying characteristic of a dosage form must be understood--from the characteristics of the individual formulation components, to how they act and interact within the formulation, and finally, to how this formulation responds in different biological environments. To achieve this, there is a wide range of analytical techniques that can be adopted to understand and elucidate the mechanics of drug delivery and drug formulation. Such methods include e.g. spectroscopic analysis, diffractometric analysis, thermal investigations, surface analytical techniques, particle size analysis, rheological techniques, methods to characterize drug stability and release, and biological analysis in appropriate cell and animal models. Whilst each of these methods can encompass a full research area in their own right, formulation scientists must be able to effectively apply these methods to the delivery system they are considering. The information in this book is designed to support researchers in their ability to fully characterize and analyze a range of delivery systems, using an appropriate selection of analytical techniques. Due to its consideration of regulatory approval, this book will also be suitable for industrial researchers both at early stage up to pre-clinical research.

Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

ANABIOTEC '92 focused on the further integration of biotechnology and analytical chemistry. The results of this symposium clearly demonstrated that a substantial progress could be reported in the application of both conventional and new analytical techniques, the latter essentially based on natural analytical tools such as biomolecules. The main themes covered during this meeting are fermentation monitoring, chromatography, instrumental analysis, biosensors and bioanalysis.