Biochemical Protozoology As A Basis For Drug Design

A compilation of articles on protozoological biochemistry which reviews the subject area and offers information on current research. Included in the Topics Covered Are Energy Metabolism Of Anaerobic Parasitic Protists, proteinases of African

This volume is the specially commissioned supplement to the journal Parasitology, volume 114.

The study of parasitic organisms at the molecular level has yielded fascinating new insights of great medical, social, and economical importance, and has pointed the way for the treatment and prevention of the diseases they cause. Biochemistry and Molecular Biology of Parasites presents an up-to-date account of this modern scientific discipline in a manner that allows and encourages the reader to place the biochemistry and molecular biology of these organisms in their biological context. The chapters are cross-referenced and grouped in an arrangement that provides a fully integrated whole, and permits the reader to create a composite of the biochemical function of these organisms. Individual chapter includes those devoted to metabolism, in both aerobic and anaerobic protozoa; antioxidant mechanisms; parasite surfaces; organelles; invasion mechanisms; and chemotherapy. The helminths are discussed not only from the point of view of their cellular biochemistry and metabolism, but also with respect to both their integrated functions such as neurochemistry, structure and functions of surfaces, and reproduction. Written by expert investigators, this book will be of interest to all experienced researchers, graduate students, and to the newcomer eager to become familiar with the biochemistry and molecular biology of parasites.

Written and edited by experts in the field, this book brings together the current state of the art in phenotypic and rational, target-based approaches to drug discovery against pathogenic protozoa. The chapters focus particularly on virtual compounds and high throughput screening, natural products, computer-assisted drug design, structure-based drug design, mechanism of action identification, and pathway modelling. Furthermore, state-of the art "omics" technologies are described and currently studied enzymatic drug targets are discussed. Mathematical, systems biology-based approaches are introduced as new methodologies for dissecting complex aspects of pathogen survival mechanisms and for target identification. In addition, recently developed anti-parasitic agents targeting particular pathways, which serve as lead compounds for further drug development, are presented.

To retain their usefulness, cultures that manufacture economically valuable products must be uncontaminated, viable, and genetically stable. Maintaining Cultures for Biotechnology and Industry gives practical advice necessary to preserve and maintain cells and microorganisms important to the biotechnology and pharmaceutical industries in ways that ensure they will continue to be able to synthesize those valuable metabolites. This book covers not just those strains currently being used but also those yet to be discovered and engineered. This text is essential for anyone working with cultures who wants to avoid the frustration of losing strains and needs to be able to devise and evaluate new strategies for preservation. Written by hands-on experts in their respective fields Contains helpful tables and protocols for preserving or maintaining cells, cultures and viruses Discusses means to preserve cells by freezing, lyophilization, drying, cyoprotection, spore storage, continuous propagation and subculturing when absolutely necessary, and others Gives information needed to test cultures for stable retention of important characteristics Gives principles needed to devise and evaluate preservation strategies for newly identified and newly engineered cells and organisms Lists culture sources for each class of organism Includes information for characterizing and monitoring recombinant organisms, especially important because of their propensity for genetic stability Discusses the history of the continually evolving field of culture preservation Examines the importance of genetically stable cultures as it relates to maintaining patent positions

Mick North's daughter Sophie was one of the children killed in the massacre at Dunblane Primary School. Dunblane: Never Forget is a personal account of Mick's life before and after the massacre and includes a critical assessement of the events that led to the tragedy and those that have followed. He begins by recalling his arrival in Stirling and how he and Barbara moved to Dunblane while awaiting the birth of their only child. A few months later Barbara was diagnosed with cancer and the family had to deal with her terminal illness. After the death of Barbara, father and daughter became an inseparable team until that dreadful day in 1996. North writes about how he and the other families dealt with public sympathy, the anti-handgun campaign, the memorial service, the television programmes, newspaper interviews and the creation of the Dunblane Memorial Garden. He describes how it felt to attend a public inquiry into the murder of his child, criticises the local police force and details the ambivalent attitude of the Dunblane community.