Bioluminescence Chemiluminescence

A compilation of authoritative articles presented in a manner which will enable them to be used as a primary source of the most significant, global research in bioluminescence and chemiluminescence.

Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical Applications of Bioluminescene and Chemiluminescence in San Diego, California on August 26-28, 1980. Part I deals with chemilunescence and excited states including topics on the spectroscopy of the solvent cage — generation and characteristics of the excited state and the three features of chemi- and bioluminescence. Part II deals with events prior to producing luminol-dependent chemiluminescence; this part also examines the effects of stimulants on membrane potential. Part III discusses bacterial bioluminescence and analyzes the properties of a lumazine protein from a bioluminescent bacterium. This part also analyzes accessory enzymes responsible for such bacterial bioluminescence. Part IV examines the chemistry of firefly bioluminescence and presents the formula of three reactions catalyzed by firefly luciferase. Part V analyzes bioluminescence from other sources such as the earthworm and land snails. Part VI discusses the applications of bioluminescence in clinical chemistry, soil science, and marine biology. Part VII deals with the future uses of bioluminescence, while Part VIII is an abstract of other papers dealing with this subject. This collection can be helpful for biologists, zoologists, micro-biologists, marine biologists, and researchers dealing with bio-chemistry and related disciplines.

Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels of light continue to push the limits of detection, allowing ready monitoring in real time of intricate subcellular processes within living cells. This book affords a glimpse of the state of the art of a rapidly advancing field, and presents to users of these methods a detailed reference of current activities in the field.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Outlines the use of luminescent reporter technologies to monitor gene expression and protein trafficking Describes the luminescence-based clinical assay technologies Details the basic biochemistry, biophysics, and chemistry of light-emitting reactions that are critical for applications Includes explanations of the instrumentation used for detection and quantification of low level light Shows the new applications of luminescence-based technologies that result largely from broad advances in recombinant DNA technologies and nonscale methods

Light-emitting reactions occur in some living organisms, and are also now extensively exploited by industry and various branches of biomedical science. Luminescence from the natural world, particularly from marine organisms, is increasingly being harnessed by genetic and chemical manipulation to enhance the quality of human life. This volume contains cutting-edge contributions from most of the world's leading researchers in this field. It presents an up-to-date compilation of the range of biomedical, strategic and ecological applications of chemiluminescence and bioluminescence. It documents and highlights the rapid advance in knowledge concerning both the mechanisms and the uses of luminescence, and covers all the important developments of recent years. Contents: Marine Bioluminescence; Firefly Bioluminescence; Chemiluminescence; Applications of Bioluminescence; Applications of Chemiluminescence; Immunoassay and DNA Probe Assays; Cellular Luminescence; Reporter Genes in Cell Biology and Analysis; Luminescence in Science and Education; Instrumentation and Imaging of Luminescence. Readership: Biomedical specialists, biochemists, marine biologists and geneticists.

The Proceedings of the 12th International Symposium on Bioluminescence (BL) and Chemiluminescence (CL) contains up-to-date information on the latest developments in BL and CL presented by scientists from around the world.Light-emitting reactions are now a vital component of many key technologies in research and in routine analytical laboratories OCo replacing radionuclides in many situations. This volume presents a compilation of the latest developments from key experts and leading-edge researchers in this area."

Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence. The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays. The proceedings have been selected for coverage in: • Biochemistry & Biophysics Citation Index™ • Chemistry Citation Index™ • Index to Scientific & Technical Proceedings® (ISTP® / ISI Proceedings) • Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings) • CC Proceedings — Engineering & Physical Sciences • CC Proceedings — Biomedical, Biological & Agricultural Sciences Contents:Bioluminescence and Mating Behavior in Pony Fish, Leiognathus nuchalis (N Azuma et al.)Importance of Firefly Luciferase C-terminal Domain in Binding of Luciferyl-Adenylate (K Ayabe et al.)Effect of Oxygen and Hydrogen Ion on the Modulation of the Bioluminescence from Luminous Bacteria (H Karatani et al.)Superoxide or Singlet Oxygen: The Chemiluminescence of Cypridina Luciferin Analogues in Photodynamic Solutions (M Bancírová & I Šnyrychová)On the Role of Singlet-Oxygen Dimol Chemiluminescence in Dioxirane Reactions (W Adam et al.)On the CIEEL Mechanism of Triggerable Dioxetanes: Does the Electron Jump or Is It Charge Transfer? (W Adam & A V Trofimov)Single-Molecule Imaging of Protein in Living Cells by Pin-Fiber Video-Microscopy (Y Hirakawa et al.)Construction of a Novel Bioluminescence Bacterial Biosensor for Real-Time Monitoring of Cytotoxic Drugs Activity (H M Alloush et al.)The Chemiluminescent Measurement of the Black and Green Tea Antioxidant Capacity and the Comparison with Their Antimicrobial Activity (M Bancírová & I Šnyrychová)Use of Bioluminescent Salmonella typhimurium DT104 to Monitor Uptake and Intracellular Survival Within a Human Cell-Line (J E Angell et al.)Tandem Bioluminescent Enzyme Immunoassay for BDNF and NT-4/5 (S Akahane et al.)Use of the Peroxyoxalate Chemiluminescent Reaction in Acetone in the Presence of Nile Red for the Analysis of Glucose (P Castro-Hartmann et al.)A New Assay for Determining Pyrophosphate Using Pyruvate Phosphate Dikinase and Its Application to DNA Analysis (H Arakawa et al.)and other papers Readership: Scientists in basic luminescene research, analytical chemists and biochemists. Keywords:Chemiluminescene;Bioluminescence;Luciferase;Luciferin;ATP;Bioanalysis;Green Fluroscent Protein (GFP);Imaging;Clinical AnalysisKey Features:Up-to-date coverage of the latest developments in bioluminescence and chemiluminescenceComprehensive coverage of fundamental and applied aspects of bioluminescence and chemiluminescenceLatest experimental procedures and protocols in bioluminescence and chemiluminescence