Biomedical Applications Of Synchrotron Infrared Microspectroscopy

Publication of a multi-author textbook on the biomedical applications of synchrotron infrared microspectroscopy was a central element in the workplan of the EU project DASIM (Diagnostic Applications of Synchrotron Infrared Microspectroscopy). The project involved nearly 70 scientists and clinicians from 9 European countries, including all synchrotron facilities that have or are planning an infrared beamline. Together with its international associates from the USA, Canada and Australia, the project brought together essentially all recognized experts in the field. The project aims were to coordinate international research effort and to disseminate the relevant information amongst biological researchers and health care professionals and this multi-author textbook was conceived as the most important measure towards the aim of dissemination. The field of biomedical applications of synchrotron IR microspectroscopy, which has recently seen unprecedented growth, is extremely interdisciplinary, involving synchrotron physicists, spectroscopists, biologists and clinicians, with associated difficulties in getting these experts to understand each other. This multi-author book, from leading world experts, presents all aspects of the field in language that all the disparate experts involved can understand. It demystifies the subject both for clinicians and biologists who find synchrotron physics difficult to understand and for physicists who find medical/biological terminology incomprehensible. The book focuses specifically on biomedical IR spectroscopy using synchrotron light sources with particular emphasis on understandable presentation of necessary background knowledge, digestible summaries of research progress and above all as a practical 'how to do it' guide for those working in or wishing to enter the field of biomedical synchrotron IR microspectroscopy and imaging. Key features of the book include:- * a 'Fundamentals' section, explaining the basics of synchrotrons and FTIR spectroscopy as well as the needs of clinicians and biologists with respect to these technologies * a 'Technical Aspects' section, going into depth on optical issues, sample preparation and study design/data analysis * case studies bringing together these 2 elements through practical examples * Raman microspectroscopy, as an alternative approach, is explored in depth * the foreword is written by Henry Mantsch and Gwynn Williams, the two undisputed experts in the fields of biomedical FTIR spectroscopy and synchrotron IR microspectroscopy respectively

Publication of a multi-author textbook on the biomedical applications of synchrotron infrared microspectroscopy was a central element in the workplan of the EU project DASIM (Diagnostic Applications of Synchrotron Infrared Microspectroscopy). The project involved nearly 70 scientists and clinicians from 9 European countries, including all synchrotron facilities that have or are planning an infrared beamline. Together with its international associates from the USA, Canada and Australia, the project brought together essentially all recognized experts in the field. The project aims were to coordinate international research effort and to disseminate the relevant information amongst biological researchers and health care professionals and this multi-author textbook was conceived as the most important measure towards the aim of dissemination. The field of biomedical applications of synchrotron IR microspectroscopy, which has recently seen unprecedented growth, is extremely interdisciplinary, involving synchrotron physicists, spectroscopists, biologists and clinicians, with associated difficulties in getting these experts to understand each other. This multi-author book, from leading world experts, presents all aspects of the field in language that all the disparate experts involved can understand. It demystifies the subject both for clinicians and biologists who find synchrotron physics difficult to understand and for physicists who find medical/biological terminology incomprehensible. The book focuses specifically on biomedical IR spectroscopy using synchrotron light sources with particular emphasis on understandable presentation of necessary background knowledge, digestible summaries of research progress and above all as a practical 'how to do it' guide for those working in or wishing to enter the field of biomedical synchrotron IR microspectroscopy and imaging. Key features of the book include:- * a 'Fundamentals' section, explaining the basics of synchrotrons and FTIR spectroscopy as well as the needs of clinicians and biologists with respect to these technologies * a 'Technical Aspects' section, going into depth on optical issues, sample preparation and study design/data analysis * case studies bringing together these 2 elements through practical examples * Raman microspectroscopy, as an alternative approach, is explored in depth * the foreword is written by Henry Mantsch and Gwynn Williams, the two undisputed experts in the fields of biomedical FTIR spectroscopy and synchrotron IR microspectroscopy respectively

Although infrared spectroscopy has been applied with success to the study of important biological and biomedical processes for many years, key advances in this vibrant technique have led to its increasing use, ranging from characterisation of individual macromolecules (DNA, RNA, lipids, proteins) to human tissues, cells and their components. Infrared spectroscopy thus has a significant role to play in the analysis of the vast number of genes and proteins being identified by the various genomic sequencing projects. Whilst this book gives an overview of the field it highlights more recent developments, such as the use of bright synchrotron radiation for recording infrared spectra, the development of two-dimensional infrared spectroscopy and the ability to record infrared spectra at ultrafast speeds. The main focus is on the mid-infrared region, since the great majority of studies are carried out in this region but there is increasing use of the near infrared for biomedical applications and a chapter is devoted to this part of the spectrum. Major advances in theoretical analysis have also enabled better interpretation of the infrared spectra of biological molecules and these are covered. The editors, Professor Andreas Barth of Stockholm University, Stockholm, Sweden and Dr Parvez I. Haris of De Montfort University, Leicester, U.K., who both have extensive research experience in biological infrared spectroscopy per se and in its use in the solution of biophysical problems, have felt it timely therefore to bring together this book. The book is intended for use both by research scientists already active in the use of biological infrared spectroscopy and for those coming new to the technique. Graduate students will also find it useful as an introduction to the technique.

This comprehensive overview of biomedical applications of vibrational spectroscopy focuses on methodologies that are most relevant to biodiagnostics. After a few introductory chapters that summarize the current status of the field, the reference covers current spectroscopic applications; new spectroscopic directions; and study design and the analysis of vibrational spectral fingerprints from complex biological and clinical samples . With chapters contributed by leading international experts, Biomedical Vibrational Spectroscopy is a core resource.

Although infrared spectroscopy has been applied with success to the study of important biological and biomedical processes for many years, key advances in this vibrant technique have led to its increasing use, ranging from characterization of individual macromolecules (DNA, RNA, lipids, proteins) to human tissues, cells and their components. Infrared spectroscopy thus has a significant role to play in the analysis of the vast number of genes and proteins being identified by the various genomic sequencing projects. Whilst this book gives an overview of the field, it highlights more recent developments, such as the use of bright synchrotron radiation for recording infrared spectra, the development of two-dimensional infrared spectroscopy and the ability to record infrared spectra at ultra fast speeds.

The latest advances in vibrational spectroscopic biomedical imaging Written by expert spectroscopists, Vibrational Spectroscopic Imaging for Biomedical Applications discusses recent progress in the field in areas such as instrumentation, detector technology, novel modes of data collection, data analysis, and various biomedical applications. This full-color volume covers various IR imaging techniques, including transmission reflection, transflection, and attenuated total reflection (ATR) imaging, and Raman imaging. The efficient use of vibrational spectroscopy in clinical applications is emphasized in this state-of-the-art guide. Coverage includes: Automated breast histopathology using mid-IR spectroscopic imaging Synchrotron-based FTIR spectromicroscopy and imaging of single algai cells and cartilage Preparation of tissues and cells for infrared and Raman spectroscopy and imaging Evanescent wave imaging sFTIR, Raman, and surface-enhanced Raman spectroscopic imaging of fungal cells Widefield Raman imaging of cells and tissues Resonance Raman imaging and quantification of carotenoid antioxidants in the human retina and skin Raman microscopy for biomedical applications--efficient diagnosis of tissues, cells, and bacteria The current sate of Raman imaging in clinical application Vibrational spectroscopic imaging of microscopic stress patterns in biomedical materials Tissue imaging with coherent anti-Stokes Raman scattering microscopy

Fourier Transform Infrared microspectroscopy (FTIR) was first developed by William Coblentz in 1905 for analytical purposes. It has been established as a powerful analytical method to analyze a wide range of materials. The most convenient way to analyze the molecular structure was to prepare KBr pellets with small amount of chemical species. Currently, the development of the Universal Attenuated Total Reflectance (UATR) allows the use of ZnSe-Diamond crystal to acquire FTIR spectra directly from the sample with no special preparation. These traditional FTIR analyses have been made with devices capable of performing single measurements, thus, providing a single IR spectrum of the sample. Recent major technological development in FTIR instrumentation was development of microscopes and imaging systems. These devices are now capable of imaging larger sample area, providing not only spectroscopic information but also spatial distributional information. In addition, the development of Focal Point Array (FPA) has made FTIR imaging an emerging area of chemical imaging research. The aim of this book is to summarize in a single document the research work that is being performed using UATR and IR imaging in selected emerging applications in plant materials and biological samples. This book provides the readers new knowledge, updates information, emerging applications, and understanding of the potential use of FTIR Microspectroscopy.

Computational Optical Biomedical Spectroscopy and Imaging covers recent discoveries and research in the field by some of the best inventors and researchers in the world. It also presents useful computational methods and applications used in optical biomedical spectroscopy and imaging. Topics covered include: New trends in immunohistochemical, genome, and metabolomics imaging Computer-aided diagnosis of interstitial lung diseases based on CT image analysis Functional near-infrared spectroscopy and its applications in neurosciences Applications of vibrational spectroscopic imaging in personal care studies Induced optical natural fluorescence spectroscopy for Giardia lamblia cysts Nanoimaging and polarimetric exploratory data analysis Fluorescence bioimaging with applications to chemistry Medical imaging instrumentation and techniques The book also discusses future applications, directions, opportunities, and challenges of optical biomedical spectroscopy and imaging in technical industry, academia, and government. This valuable resource introduces key concepts of computational methods used in optical biomedical spectroscopy and imaging in a manner that is easily understandable to beginners and experts alike.