Cell Based Biosensors

Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations help clarify key topics throughout the book.

This handbook is an interdisciplinary and comprehensive reference covering all aspects of cell biosensors. It is divided into four main sections which are led and organized by numerous international experts. The scope of coverage includes: Fundamentals and genetics for biosensor applications Transducers, Materials and Systems Markets, innovation and education Application of biosensors in business Biosensor research is an exciting hybrid world where biologists, chemists, physicists, engineers and computer engineers come together. This handbook will serve as an invaluable living resource for all researchers in academia and industry working with cell biosensors.

Advances in genomics and combinatorial chemistry during the past two decades inspired innovative technologies and changes in the discovery and pre-clinical development paradigm with the goal of accelerating the process of bringing therapeutic drugs to market. Written by William Kisaalita, one of the foremost experts in this field, 3D Cell-Based Bio

Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology

This book covers the full scope of biochemical sensors and offers a survey of the principles, design and applications of the most popular types of biosensing devices. It is presented in 19 chapters, written by 20 distinguished scientists as well as their co-workers. The topics include the design of signal transducers, signal tags and signal amplification strategies, the structure of biosensing interfaces with new biorecognition elements such as aptamers and DNAzymes, and different newly emerging nanomaterials such as Au nanoclusters, carbon nitride, silicon, upconversion nanoparticles and two-dimensional materials, and the applications in wearable detections, biofuel cells, biomarker analyses, bioimaging, single cell analysis and in vivo sensing.By discussing recent advances, it is hoped this book will bridge the common gap between research literature and standard textbooks. Research into biochemical sensors and their biomedical applications is proceeding in a number of exciting directions, as reflected by the content. This book is published in honor of the 90th birthday of Professor Shaojun Dong, who performed many pioneering studies on modified electrodes and biochemical sensors.

The main challenge in biosensor development is their application for various practical tasks to provide a continuous and reliable flow of information about the indicators of natural and industrial processes and the surroundings, so enabling adequate feedback and control. Biosensors can provide essential information, as the quality of life depends mainly on our knowledge about what we breathe, what we eat and how our bodies are able to metabolize the material, which we contact. This book includes 14 chapters, written by 52 authors and is focused on the applications of biosensors for monitoring the parameters of environment, the quality of food and biomarkers of health.

SOME ADVANTAGES OF CELL-BASED BIOSENSORS: Isolation, purification, and immobilization of enzymes are often very difficult; these can be omitted with cell-based biosensors. Some enzymes can lose their activity during isolation or immobilization; this risk is eliminated by the use of whole cells. Enzymes in the cell's natural environment are usually extremely stable. Multi-step enzyme reactions in intact cells can be used, making it possible to avoid the preparation of complicated artificial multi-enzyme systems. Coenzymes and activators are often present in the cells, and thus, it is not necessary to add them into the system; the cell itself usually cares for their effective regeneration.

Real-time and reliable detection of molecular compounds and bacteria is essential in modern environmental monitoring. For rapid analyses, biosensing devices combining high selectivity of biomolecular recognition and sensitivity of modern signal-detection technologies offer a promising platform. Biosensors allow rapid on-site detection of pollutants and provide potential for better understanding of the environmental processes, including the fate and transport of contaminants.This book, including 12 chapters from 37 authors, introduces different biosensor-based technologies applied for environmental analyses.