Determining Mycotoxins And Mycotoxigenic Fungi In Food And Feed

Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi – pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area. Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques. With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain. Focuses on the essentials of mycotoxin determination, covering sampling, sample preparation, clean-up and key determination techniques Documents quality assurance and official methods and performance criteria for determining mycotoxins in food and feed Explores the processes of determining mycotoxigenic fungi in food and feed including the identification of genes and gene clusters

This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts.

Mycotoxins are toxins produced by aerobic, microscopic fungus under special conditions of moisture and temperature. They colonize in a variety of foods from harvest to the grocer. Mycotoxins have gained world wide interest in recent years with the revelation of the effect of these toxins on health. A current example is the presence of ochratoxin A, a human carcinogen and nephrotoxin, in wines. The increased concern about fruit safety has led to increased studies throughout the world and enhanced awareness for stringent regulations governing mycotoxin limits in food. Presented in three defined sections, this is the first book to provide comprehensive analysis of the main mycotoxins contaminating fruits and vegetables and their derived products. The first section provides a safety evaluation of mycotoxins in fruits and vegetables, details regarding factors affecting mycotoxin production and diffusion in the fruit tissue, and recent methods for detection of mycotoxigenic fungi and mycotoxins produced by the fungi. The second part takes a critical look at the main individual mycotoxins and the third section focuses on approaches for prevention and control. * The first book dedicated to mycotoxins in fruits and vegetables * Presents mycological, mycotoxicological and phytopathological aspects of fruits and vegetables * Includes an analysis of detection, prevention and control methods for mycotoxigenic fungi and the mycotoxins they produce * Provides a complete risk assessment and safety evaluation of mycotoxins in perishable produce

Anti-Mycotoxin Strategies for Food and Feed Up-to-date information on mycotoxigenic fungi and toxins, with methods to control them in food and feed Anti-Mycotoxin Strategies for Food and Feed focuses on strategies to control the health risk of mycotoxins associated with cereals, pulses, and vegetables during post and pre harvest stages, in addition to foods, food-based products, and feeds. Each chapter is carefully designed to offer information elucidating various strategies, which include physical, chemical, and biological methods. The book provides a combined approach of advanced techniques used against mycotoxigenic fungi, which is helpful in studying the strategies for different mycotoxins. Special attention has been paid to diseases caused by mycotoxigenic fungi and their destructive effect on either preharvest, post-harvest, or storage, and their respective toxins which pose a long-term health risk to humans and animals, if contaminated food or feed enter our food chain. The primary focus of the text is on the recent and advanced approaches and strategies including control, detoxification, degradation and binding methods, with graphical representations of protocols with supporting tables and figures. Anti-Mycotoxin Strategies discusses specific sample topics such as: Advanced anti-fumonisin strategies and strategies for the control of aflatoxigenic Aspergillus species in contaminated food and feed Innovative strategies to decontaminate Ochratoxin A and Zearalenone. Effects of toxic citrinin, patulin and Ergot alkaloids in animals, birds, and humans, and effective mitigation strategies as its control measures. Detoxification and Decontamination strategies of Deoxynivalenol an emetic toxin, Trichothecenes, T-2/HT-2 toxin and nivalenol associated in food and feed as contaminants. Presenting and discussing mycotoxin management strategies at length and enabling readers to reduce or eliminate health hazards to humans and animals, Anti-Mycotoxin Strategies is an essential resource for mycologists, mycotoxicologists, Microbiologists, Food technology professionals in the food, seed, and feed industries, scientists, students, researchers, and farmers / agriculturists.

This Edited Volume Mycotoxins - Impact and Management Strategies is a collection of reviewed and relevant research chapters, offering a comprehensive overview of recent developments in the field of Mycotoxicology. The book comprises of single chapters authored by various researchers and edited by an expert active in this research area. This book is divided into three sections. Section 1 consists of one chapter that gives an overview of the socioeconomic impact of mycotoxins. Section 2 has five chapters that address the prevention and control of aflatoxins both at pre- and post-harvest stages. Section 3 has two chapters that deal with health impact and control in the poultry industry. This publication aims at providing a thorough overview of the latest research efforts in the field and opens new possible research paths for further novel developments in addressing the problem of mycotoxins.

Mycotoxins, toxic compounds produced by fungi, pose a significant contamination risk in both animal feed and foods for human consumption. With its distinguished editors and international team of contributors, Mycotoxins in food summarises the wealth of recent research on how to assess the risks from mycotoxins, detect particular mycotoxins and control them at differing stages in the supply chain. Part one addresses risk assessment techniques, sampling methods, modelling and detection techniques used to measure the risk of mycotoxin contamination and the current regulations governing mycotoxin limits in food. Part two looks at how the risk of contamination may be controlled, with chapters on the use of HACCP systems and mycotoxin control at different stages in the supply chain. Two case studies demonstrate how these controls work for particular products. The final section details particular mycotoxins, from ochratoxin A and patulin to zearalenone and fumonisins. Mycotoxins in food is a standard reference for all those concerned with ensuring the safety of food. Discusses the wealth of recent research in this important area Covers risk assessment, detection of particular mycotoxins and how to control them throughout the supply chain Describes how the risk of contamination can be controlled, including the use of HACCP systems

The production of animal feed increasingly relies on the global acquisition of feed material, increasing the risk of chemical and microbiological contaminants being transferred into food-producing animals. Animal feed contamination provides a comprehensive overview of recent research into animal feed contaminants and their negative effects on both animal and human health. Part one focuses on the contamination of feeds and fodder by microorganisms and animal by-products. Analysis of contamination by persistent organic pollutants and toxic metals follows in part two, before the problem of natural toxins is considered in part three. Veterinary medicinal products as contaminants are explored in part four, along with a discussion of the use of antimicrobials in animal feed. Part five goes on to highlight the risk from emerging technologies. Finally, part six explores feed safety and quality management by considering the safe supply and management of animal feed, the process of sampling for contaminant analysis, and the GMP+ feed safety assurance scheme. With its distinguished editor and international team of expert contributors, Animal feed contamination is an indispensable reference work for all those responsible for food safety control in the food and feed industries, as well as a key source for researchers in this area. Provides a comprehensive review of research into animal feed contaminants and their negative effects on both animal and human health Examines the contamination of feeds and fodder by microorganisms and animal by-products Analyses contamination by persistant organic pollutants, toxic metals and natural toxins

The first book to cover this fast developing field, Masked Mycotoxins in Food will provide a full overview of the issues relating to the toxicology of masked mycotoxins present in food products. Mycotoxins are naturally occurring chemicals produced by moulds that can grow on crops and foodstuffs. Masked mycotoxins are modified mycotoxins, due to this modification many cannot be detected using standard analytical techniques, for example HPLC and ELISA, and further research is needed to understand the health risks and threats from these modified compounds.Masked mycotoxin research is an area of toxicological research that has gained significant interest and momentum in recent years. The aim of this book is to provide a full picture of the topic, from the masked mycotoxin formation in plants to their catabolic fate in humans. The book also provides new insights and will highlight possible gaps in the knowledge base of this relatively new area. Edited and written by World renowned experts working within the field, this book is of interest to toxicologists and biochemists, but also food scientists and agricultural researchers working in industry and academia.