Genetic And Metabolic Engineering For Improved Biofuel Production From Lignocellulosic Biomass

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Describes technological advancements for bioethanol production from lignocellulosic waste Provides a roadmap for the production and utilization of 2G biofuels Introduces the strategic role of metabolic engineering in the development of 2G biofuels Discusses technological advancements, life cycle assessment and prospects Explores novel potential lignocellulosic biomass for 2G biofuels

This book reviews state of the art regarding strategies for generating and improving microbial strains designed for utilizing renewable raw materials. It discusses methods for genetically engineering of thermophilic bacteria, Saccharomyces cerevisiae, Escherichia coli and Zymomonas mobilis, as well as approaches for obtaining useful products from these renewable raw materials based on biotechnological processes using microbes to chemically transform them. However, the efficient transformation of lignocellulosic biomass or glycerol to useful products represents a major challenge: Biomass has to be treated physically and chemically to release a mixture of sugars that potentially can be employed by the microbial production strains. These hydrolytic treatments result in diverse toxic compounds being generated and released, that negatively impact strain performance. Furthermore, most of the commonly used industrial microbes do not have the natural capacity to efficiently utilize and transform the generated sugar mixtures or glycerol. The microbial species reviewed in this book possess particular advantages as production strains and are currently employed for the synthesis of numerous biofuels and chemicals. The book reviews the general and strain-specific genetic engineering strategies for the improvement of sugar mixtures and glycerol catabolism. The issue of lignocellulosic hydrolysate toxicity is addressed in several chapters, where genetic engineering and adaptive laboratory evolution strategies are reviewed and discussed. The objective of this book is to provide the current knowledge regarding strategies for the generation and improvement of microbial strains designed for the transformation of renewable raw materials into useful products. This book aims to become a reference for researchers and students working in this field.

Converting biomass to biofuels involves hydrolyzing cellulose to sugars using cost-intensive commercial enzymes – an expensive step that makes large-scale production economically non-viable. As such, there is a need for low-cost bioprocessing. This book critically evaluates the available bioprocessing technologies for various biofuels, and presents the latest research in the field. It also highlights the recent developments, current challenges and viable alternative approaches to reduce the overall cost of producing biofuels.

The global warming problem is becoming critical year by year, causing climate disaster all over the world, where it has been believed that the CO2 gas emitted from the factories and the burning of fossil fuels may be one of the reasons of global warming. Moreover, the global stock of fossil fuels is limited, and may run out soon within several tens of years. Although wind, geo-thermal, and tide energies have been considered as clean energy sources, those depend on the land or sea locations and subject to the climate change. Biofuel and biochemical production from renewable bio-resources has thus been paid recent attention from environmental protection and energy production points of view, where the current chemical and energy producing plants can be also utilized with slight modification. The so-called 1st generation biofuels have been produced from corn starch and sugarcane in particular in USA and Brazil. However, this causes the problem of the so-called "food and energy issues" as the production scale increases. The 2nd generation biofuel production from lingo-cellulosic biomass or wastes has thus been paid recent attention. However, it requires energy intensive pretreatment for the degradation of lingo-cellulosic biomass, and the fermentation is slow due to low growth rate, and thus the productivity of biofuels and bio-chemicals is low. The 3rd generation biofuel production from photosynthetic organisms such as cyanobacteria and algae has been also paid attention, because such organisms can grow with only sun light and CO2 in the air, but the cell growth rate and thus the productivity of the fuels is significantly low. The main part or core of such production processes is the fermentation by micro-organisms. In particular, it is critical to properly understand the cell metabolism followed by the efficient metabolic engineering. The book gives comprehensive explanation of the cell metabolism and the metabolic regulation mechanisms of a variety of micro-organisms. Then the efficient metabolic engineering approaches are explained to properly design the microbial cell factories for the efficient cell growth and biofuel and biochemical production.

Advances in Lignocellulosic Biofuel Production Systems focuses on general topics such as novel pretreatment strategies, lignocellulosic biomass as a suitable feedstock for biofuels, lifecycle assessment and integrated biorefineries. Furthermore, the book focuses on more advanced topics such as genetically engineered feedstocks, metabolically engineered microbes, bioreactor design and configuration, cell immobilization strategies, artificial intelligence applications and nanotechnology. This book will guide readers through all aspects of lignocellulosic biofuel production rather than simply covering a single topic. Provides information on the most advanced and innovative technologies for biomass valorization, including the design and configuration of bioreactors Identifies research gaps in the application of artificial intelligence, nanotechnology, cell immobilization, metabolic engineering, kinetic assessment and genetically engineered feedstocks for enhancing lignocellulosic bioprocessing and biofuel yield Presents a global overview of the supply chain for biofuels production from lignocellulosic biomass Includes techno-economic analysis, along with environmental and socioeconomic impact assessments of various technologies

This book compiles research aspects of second-generation (2G) biofuel production derived specifically from lignocellulose biomass using biorefinery methods. It focuses on the valorization of different sources of 2G biofuels and their relative importance. The constituents of lignocelluloses and their potential characteristics different methods of treating lignocellulose, various means of lignocellulose bioconversion, and biofuel production strategies are discussed. Features: Describes technological advancements for bioethanol production from lignocellulosic waste. Provides the roadmap for the production and utilization of 2G biofuels. Introduces the strategic role of metabolic engineering in the development of 2G biofuels. Discusses technological advancements, life cycle assessment, and prospects. Explores the novel potential lignocellulosic biomass for 2G biofuels. This book is aimed at researchers and professionals in renewable energy, biofuel, bioethanol, lignocellulose conversion, fermentation, and chemical engineering.