Engineering Of Microorganisms For The Production Of Chemicals And Biofuels From Renewable Resources

This book reviews state of the art regarding strategies for generating and improving microbial strains designed for utilizing renewable raw materials. It discusses methods for genetically engineering of thermophilic bacteria, Saccharomyces cerevisiae, Escherichia coli and Zymomonas mobilis, as well as approaches for obtaining useful products from these renewable raw materials based on biotechnological processes using microbes to chemically transform them. However, the efficient transformation of lignocellulosic biomass or glycerol to useful products represents a major challenge: Biomass has to be treated physically and chemically to release a mixture of sugars that potentially can be employed by the microbial production strains. These hydrolytic treatments result in diverse toxic compounds being generated and released, that negatively impact strain performance. Furthermore, most of the commonly used industrial microbes do not have the natural capacity to efficiently utilize and transform the generated sugar mixtures or glycerol. The microbial species reviewed in this book possess particular advantages as production strains and are currently employed for the synthesis of numerous biofuels and chemicals. The book reviews the general and strain-specific genetic engineering strategies for the improvement of sugar mixtures and glycerol catabolism. The issue of lignocellulosic hydrolysate toxicity is addressed in several chapters, where genetic engineering and adaptive laboratory evolution strategies are reviewed and discussed. The objective of this book is to provide the current knowledge regarding strategies for the generation and improvement of microbial strains designed for the transformation of renewable raw materials into useful products. This book aims to become a reference for researchers and students working in this field.

Discover biomolecular engineering technologies for the production of biofuels, pharmaceuticals, organic and amino acids, vitamins, biopolymers, surfactants, detergents, and enzymes In Biomolecular Engineering Solutions for Renewable Specialty Chemicals, distinguished researchers and editors Drs. R. Navanietha Krishnaraj and Rajesh K. Sani deliver a collection of insightful resources on advanced technologies in the synthesis and purification of value-added compounds. Readers will discover new technologies that assist in the commercialization of the production of value-added products. The editors also include resources that offer strategies for overcoming current limitations in biochemical synthesis, including purification. The articles within cover topics like the rewiring of anaerobic microbial processes for methane and hythane production, the extremophilic bioprocessing of wastes to biofuels, reverse methanogenesis of methane to biopolymers and value-added products, and more. The book presents advanced concepts and biomolecular engineering technologies for the production of high-value, low-volume products, like therapeutic molecules, and describes methods for improving microbes and enzymes using protein engineering, metabolic engineering, and systems biology approaches for converting wastes. Readers will also discover: A thorough introduction to engineered microorganisms for the production of biocommodities and microbial production of vanillin from ferulic acid Explorations of antibiotic trends in microbial therapy, including current approaches and future prospects, as well as fermentation strategies in the food and beverage industry Practical discussions of bioactive oligosaccharides, including their production, characterization, and applications In-depth treatments of biopolymers, including a retrospective analysis in the facets of biomedical engineering Perfect for researchers and practicing professionals in the areas of environmental and industrial biotechnology, biomedicine, and the biological sciences, Biomolecular Engineering Solutions for Renewable Specialty Chemicals is also an invaluable resource for students taking courses involving biorefineries, biovalorization, industrial biotechnology, and environmental biotechnology.

Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. Covers genetic engineering approaches for higher cellulase production from fungi Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds Describe different bioreactors used in different steps of biomass-based biofuel production Outlines future prospects and potential of biofuel production from lignocellulosic biomass

Nature offers abundant renewable resources that can be used to partially replace fossil fuels and commodity chemicals but issues of cost, technology readiness levels, and compatibility with existing distribution networks remain huge challenges. Cellulosic ethanol and biodiesel are the most immediately obvious target fuels, with hydrogen, methane and butanol as other potentially viable products. This book continues to bridge the technology gap and focus on critical aspects of lignocellulosic biomolecules and the respective mechanisms regulating their bioconversion to liquid fuels into energy and value-added products of industrial significance. This book is a collection of reviews elucidating several broad-ranging areas of progress and challenges in the utilization of sustainable resources of renewable energy, especially in biofuels. This book comes just at a time when government and industries are accelerating their efforts in the exploration of alternative energy resources, with expectations of the establishment of long-term sustainable alternatives to petroleum-based liquid fuels. Apart from liquid fuel this book also emphasizes the use of sustainable resources for value-added products, which may help in revitalizing the biotechnology industry at a broader scale. This book also provides a comprehensive review of basic literature and advance research methodologies to graduate students studying environmental microbiology, chemical engineering, bio-economy and microbial biotechnology.

The book provides an overview on various microorganisms and their industrialization in energy conversion, such as ethanol fermentation, butanol fermentation, biogas fermentation and fossil energy conversion. It also covers microbial oil production, hydrogen production and electricity generation. The content is up to date and suits well for both researchers and industrial audiences.

RENEWABLE ENERGY INNOVATIONS This critical text, designed for microbiologists, biotechnologists, entrepreneurs, process engineers, chemical engineers, electrical engineers, physicists, and environmentalists, assesses the current knowledge about lab-scale and large-scale production of renewable and sustainable fuels, chemicals, and materials. Global warming is having a huge impact on the world’s ecosystem. Glaciers have shrunk, ice on rivers and lakes is breaking up early, and plant and animal ranges have relocated. On a worldwide scale, the threat posed by climate change and pollution is obvious. A green and sustainable future necessitates using renewable resources to produce fuels, chemicals, and materials. This book investigates diverse bioprocesses that are crucial to everyday life, including the key concerns regarding the generation of biofuels, energy, and food securities, along with waste management. Commercial interest in biotechnological processes has risen to produce pharmaceuticals, health supplements, foodstuffs, biofuels, and chemicals using a biocatalyst such as enzymes, microorganisms, plant cells, or animal cells in a bioreactor. The sustainability of renewable biomass, replacement of depleted fossil fuels, and the mitigation of greenhouse gas emissions from the existing chemical and oil industries are the key benefits of switching to bioproducts. This book discusses bioprocessing to produce biofuels, biobased chemicals, bioproducts, and biomass biorefinery processes. This involves designing novel pretreatment and fractionation technologies for lignocellulose biomass into cellulose, hemicellulose, and lignin and the conversion of these streams into biofuels and biobased chemicals via biochemical and thermochemical routes. This book also covers the advancement of oleaginous microorganisms for biofuels and nutraceutical, biological wastewater treatment. Written and edited by authors from leading biotechnology research groups from across the world, this exciting new volume covers all of these technologies, including the basic concepts and the problems and solutions involved with the practical applications in the real world. Whether for the veteran engineer or scientist, student, manager, or another technician working in the field, this volume is essential for any library.

This book provides the vision of a successful biorefinery—the lignocelluloic biomass needs to be efficiently converted to its constituent monomers, comprising mainly of sugars such as glucose, xylose, mannose and arabinose. Accordingly, the first part of the book deals with aspects crucial for the pretreatment and hydrolysis of biomass to give sugars in high yield, as well as the general aspects of bioprocessing technologies which will enable the development of biorefineries through inputs of metabolic engineering, fermentation, downstream processing and formulation. The second part of the book gives the current status and future directions of the biological processes for production of ethanol (a biofuel as well as an important commodity raw material), solvents (butanol, isobutanol, butanediols, propanediols), organic acids (lactic acid, 3-hydroxy propionic acid, fumaric acid, succinic acid and adipic acid), and amino acid (glutamic acid). The commercial production of some of these commodity bioproducts in the near future will have a far reaching effect in realizing our goal of sustainable conversion of these renewable resources and realizing the concept of biorefinery. Suitable for researchers, practitioners, graduate students and consultants in biochemical/ bioprocess engineering, industrial microbiology, bioprocess technology, metabolic engineering, environmental science and energy, the book offers: Exemplifies the application of metabolic engineering approaches for development of microbial cell factories Provides a unique perspective to the industry about the scientific problems and their possible solutions in making a bioprocess work for commercial production of commodity bioproducts Discusses the processing of renewable resources, such as plant biomass, for mass production of commodity chemicals and liquid fuels to meet our ever- increasing demands Encourages sustainable green technologies for the utilization of renewable resources Offers timely solutions to help address the energy problem as non-renewable fossil oil will soon be unavailable

Biotechnology for Biofuel Production and Optimization is the compilation of current research findings that cover the entire process of biofuels production from manipulation of genes and pathways to organisms and renewable feedstocks for efficient biofuel production as well as different cultivation techniques and process scale-up considerations. This book captures recent breakthroughs in the interdisciplinary areas of systems and synthetic biology, metabolic engineering, and bioprocess engineering for renewable, cleaner sources of energy. Describes state-of-the-art engineering of metabolic pathways for the production of a variety of fuel molecules Discusses recent advances in synthetic biology and metabolic engineering for rational design, construction, evaluation of novel pathways and cell chassis Covers genome engineering technologies to address complex biofuel-tolerant phenotypes for enhanced biofuel production in engineered chassis Presents the use of novel microorganisms and expanded substrate utilization strategies for production of targeted fuel molecules Explores biohybrid methods for harvesting bioenergy Discusses bioreactor design and optimization of scale-up