Gold And Silver Staining

Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know about the various staining methods, their useful applications, and the advantages and drawbacks of each process. Gold and Silver Staining: Techniques in Molecular Morphology provides a timely description of approaches, methods, protocols, and applications. The contributors cover the latest developments and a wide range of applications from highly sensitive detection of antigens to single copy detection of DNA and RNA. Some of the authors are "living legends" in the field and bring their expertise and experience to this fine collaboration. Written in one consistent style, each chapter includes a concise, but comprehensive introduction, step-by-step protocols with technical hints, and a discussion of results and critical steps. What differentiates this book from all others like it, is the status of the editors, who have worked on this technique from its inception and have produced innumerable publications on the topic. The other distinguishing feature is that all of the contributors are amongst the absolute foremost leaders from the United States and Europe. Gold and Silver Staining: Techniques in Molecular Morphology presents a complete overview and detailed descriptions of this technique that allows the visualization of molecules that have never been localized before and with hitherto unknown sensitivity. Not only does this book provide an excellent review of this field, but it also serves as a lab manual for those who want to carry out this technique in their laboratory and clinical work. Armed with this information, advances in this powerful field of research will ensue.

This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Nanotechnology is the key technology of the 21st century. The possibility to exploit the structures and processes of biomolecules for novel functional materials, biosensors, bioelectronics and medical applications has created the rapidly growing field of nanobiotechnology. Designed as a broad survey of the field, this book combines contributions from bioorganic and bioinorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of current and future developments. It is divided into four main sections: * Interphase Systems * Protein-based Nanostructures * DNA-based Nanostructures * Nanoanalytics Each chapter describes in detail currently available methods and contains numerous references to the primary literature, making this the perfect "field guide" for chemists, biologists and materials scientists who want to explore the fascinating world of nanobiotechnology.

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling

Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more pre

While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.