Insect Pathology

Insect Pathology is designed for a broad spectrum of readers. Is should be useful to students, lecturers, and researchers requiring information about the principles in insect pathology and the biology of pathogens. It should serve as a resource for specialists to learn about other insect pathogen systems, for generalists to become aware of advances in insect pathology, and for scientists and students, beginning or otherwise, interested in learning about insect pathology. This book was originally intended to update the 1949 test by E. A. Steinhaus entitled Principles of Insect Pathology. The purpose for this book was twofold: To serve (1) as a text for an insect pathology and/or biological control class and (2) as a comprehensive reference source. Because this book summarizes much of the available information, its usefulness as a textbook for an insect pathology class is apparent. Although the literature citations are extensive, they are far from complete. The literature in insect pathology is voluminous and for the past decade has been expanding at an almost exponential rate. A complete review of the literature is beyond the scope of the book, and an omission of a reference does not preclude its importance. Our citations, however, should serve as a good starting point for those who wish to obtain further information. We have attempted to cover equally all subdisciplines, but shortcomings are unavoidable. For these, we take full responsibility.

Principles of Insect Pathology, a text written from a pathological viewpoint, is intended for graduate-level students and researchers with a limited background in microbiology and in insect diseases. The book explains the importance of insect diseases and illuminates the complexity and diversity of insect-microbe relationships. Principles of Insect Pathology combines the disciplines of microbiology (virology, bacteriology, mycology, protozoology), pathology, and immunology within the context of the insect host, providing a format which is understandable to entomologists, microbiologists, and comparative pathologists.

Insect Pathology, 2nd ed. Fernando E. Vega and Harry K. Kaya, Editors Foreword May Berenbaum Chapter 1. Scope and Basic Principles of Insect Pathology Harry K. Kaya and Fernando E. Vega Chapter 2 - History of Insect Pathology Elizabeth W. Davidson Chapter 3 - Principles of Epizootiology and Microbial Control David I. Shapiro-Ilan, Denny Bruck, and Lawrence A. Lacey Chapter 4 - Baculoviruses and Other Occluded Insect Viruses Robert Harrison and Kelli Hoover Chapter 5 - RNA Viruses Infecting Pest Insects Yan Ping Chen, James J. Becnel, and Steven M. Valles Chapter 6 - Fungal Entomopathogens Fernando E. Vega, Nicolai V. Meyling, Janet Jennifer Luangsa-ard, and Meredith Blackwell Chapter 7 - Microsporidian Entomopathogens Leellen F. Solter, James J. Becnel, and David H. Oi Chapter 8 - Bacterial Entomopathogens Juan Luis Jurat-Fuentes and Trevor Jackson Chapter 9 - Wolbachia Infections in Arthropod Hosts Grant Hughes and Jason L. Rasgon Chapter 10 - Protistan Entomopathogens Carlos E. Lange ...

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains

This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.

Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens New edition fully updated to address changes in the taxonomy of the vast majority of taxa Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques Provides extensive supplemental literature and recipes for media, fixatives and stains

Insect Pathology, Third Edition offers the most comprehensive research on the topic of insect pathology, spanning the diversity of insects with a more diverse array of microbes. This book provides a full update on new approaches for studying the genetics, development, evolution, and ecology of insect pathogens. Written by leading researchers and experts, the book includes coverage of revolutionary genome editing tools, such as CRISPR-Cas9, which have been developed for multiple insect pathogen systems and provides insights into the molecular underpinnings of insect pathogens and their hosts. This book also updates modern molecular techniques, “omics approaches, and newly applied findings. These new and revised topics are critical for understanding current perspectives in the field, making this book a needed reference for insect pathologists, entomologists, microbiologists, mycologists, nematologists, protistologists, ecologists, and practitioners of biological control of insect pests. New and long-time students of insect pathology will also find this new edition useful. Revised to include information on new molecular approaches and technologies like CRISPR-Cas9 Highlights the importance of advances in genomics, transcriptomics, and proteomics Outlines the history and importance of all major insect pathogens