Life At The Nanoscale

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Embracing Biology's Complexity, At Last -- Learning by Making: DNA and Protein Nanotechnology -- Nano in Medicine -- Recreating Tissues and Organs -- Conclusions : Life Changes Everything.

"Increasingly, scientists are gaining control over matter at the nanometer scale. Spearheaded by physical scientists operating at the interfaces of physics and biology (such as the author herself), advances in nanoscience and technology are transforming how we think about life and treat human health. This is due to a convergence of size. To do medicine, one must understand and be able to reach the nanoscale environment of healthy cells in tissues and organs, as well as other nano-sized building blocks that constitute a living organism, such as proteins and DNA. The ground-breaking advances being made at the frontiers of nanoscience and -technology, specifically in the areas of biology and medicine, are the subject of this short, popular-level book. Chapter 1 describes how nanotechnology and quantitative methods in biology are progressively being deployed to embrace life in all its multiscale, hierarchical intricacy and multiplicity. Chapters 2 through 4 review how bioinspired and biomimetic nanostructures and nanomachines are being created and integrated into strategies aimed at solving specific medical problems. In particular, Chapter 2 summarizes how scientists are seeking to build artificial nanostructures using both biological molecules and the organizational principles of biology. Chapter 3 gives an account of how nanotechnology is being used to develop drug-delivery strategies that specifically target cancer cells and tumors to improve the efficacy of current cancer chemotherapies. Chapter 4 reviews the science of one of the most potentially transformative scientific fields: tissue engineering. In a concluding chapter (Chapter 5), Contera reviews how nanotechnology, biology, and medicine will continue fusing with other sciences and technologies - incorporating more mathematical and computational modelling, as well as AI and robotics. Nanoscale devices will be used to learn biology; and biology will be used to inspire increasingly sophisticated "transmaterial" devices that mimic some of the characteristics of biology and incorporate new features that are not available in the biological world. The effects on human health and longevity will be profound. In a more personal epilogue, Contera describes the crossroads at which we find ourselves. Accessing our own biology evokes a mixture of possibility and dread. However, Contera maintains that we can create a positive transmaterial world for the benefit of humankind, and she describes ways in which scientists are proactively engaging with the public, politicians, industry, and entrepreneurs, as well as the media and the arts, to communicate the power and risks of new advances and to influence the ways in which new technologies will affect our future"--

A small revolution is remaking the world. The only problem is, we can’t see it. This book uses dazzling images and evocative descriptions to reveal the virtually invisible realities and possibilities of nanoscience. An introduction to the science and technology of small things, No Small Matter explains science on the nanoscale. Authors Felice C. Frankel and George M. Whitesides offer an overview of recent scientific advances that have given us our ever-shrinking microtechnology—for instance, an information processor connected by wires only 1,000 atoms wide. They describe the new methods used to study nanostructures, suggest ways of understanding their often bizarre behavior, and outline their uses in technology. This book explains the various means of making nanostructures and speculates about their importance for critical developments in information processing, computation, biomedicine, and other areas. No Small Matter considers both the benefits and the risks of nano/microtechnology—from the potential of quantum computers and single-molecule genomic sequencers to the concerns about self-replicating nanosystems. By making the practical and probable realities of nanoscience as comprehensible and clear as possible, the book provides a unique vision of work at the very boundaries of modern science.

Life is an enduring mystery. Yet, science tells us that living beings are merely sophisticated structures of lifeless molecules. If this view is correct, where do the seemingly purposeful motions of cells and organisms originate? In Life's Ratchet, physicist Peter M. Hoffmann locates the answer to this age-old question at the nanoscale. Below the calm, ordered exterior of a living organism lies microscopic chaos, or what Hoffmann calls the molecular storm -- specialized molecules immersed in a whirlwind of colliding water molecules. Our cells are filled with molecular machines, which, like tiny ratchets, transform random motion into ordered activity, and create the "purpose" that is the hallmark of life. Tiny electrical motors turn electrical voltage into motion, nanoscale factories custom-build other molecular machines, and mechanical machines twist, untwist, separate and package strands of DNA. The cell is like a city -- an unfathomable, complex collection of molecular workers working together to create something greater than themselves. Life, Hoffman argues, emerges from the random motions of atoms filtered through these sophisticated structures of our evolved machinery. We are agglomerations of interacting nanoscale machines more amazing than anything in science fiction. Rather than relying on some mysterious "life force" to drive them -- as people believed for centuries -- life's ratchets harness instead the second law of thermodynamics and the disorder of the molecular storm. Grounded in Hoffmann's own cutting-edge research, Life's Ratchet reveals the incredible findings of modern nanotechnology to tell the story of how the noisy world of atoms gives rise to life itself.

This book presents the perspectives of nanotechnology educators from around the world. Experts present the pressing challenges of teaching nanoscience and engineering to students in all levels of education, postsecondary and informal environments. The book was inspired by the 2014 NSF workshop for Nanoscience and Engineering Education. Since nanotechnology is a relatively new field, authors present recommendations for designing nanotechnology education programs. The chapters describe methods to teach specific topics, such as probe microscopy, size and scale, and nanomaterial safety, in classrooms around the world. Other chapters describe the ways that organizations like NNIN and the NISE Network have influenced informal nanotechnology education. Information technology plays a growing role in all types of education and several chapters are devoted to describing ways how educators can use online curricula for teaching nanotechnology to students from preschool to graduate school.

Enthusiasts look forward to a time when tiny machines reassemble matter and process information with unparalleled power and precision. But is their vision realistic? Where is the science heading? As nanotechnology (a new technology that many believe will transform society in the next one hundred years) rises higher in the news agenda and popular consciousness, there is a real need for a book which discusses clearly the science on which this technology will be based. Whilst it is most easy to simply imagine these tiny machines as scaled-down versions of the macroscopic machines we are all familiar with, the way things behave on small scales is quite different to the way they behave on large scales. Engineering on the nanoscale will use very different principles to those we are used to in our everyday lives, and the materials used in nanotehnology will be soft and mutable, rather than hard and unyielding. "Soft Machines" explains in a lively and very accessible manner why the nanoworld is so different to the macro-world which we are all familiar with. Why does nature engineer things in the way it does, and how can we learn to use these unfamiliar principles to create valuable new materials and artefacts which will have a profound effect on medicine, electronics, energy and the environment in the twenty-first century. With a firmer understanding of the likely relationship between nanotechnology and nature itself, we can gain a much clearer notion of what dangers this powerful technology may potentially pose, as well as come to realise that nanotechnology will have more in common with biology than with conventional engineering.