Manual Of Intracytoplasmic Sperm Injection In Human Assisted Reproduction

"Not everything in medical science has a clear beginning. The first realization of infertility and putative remedies remain shrouded in contextual history, but likely goes back to the dawn of our species, well before there was a written record. Childlessness was, and is still, considered a burden in some communities"--

Manual of Assisted Reproductive Technologies and Clinical Embryology aims to discuss the relevance of science of reproductive biology in modern-day Assisted Reproductive Technologies and their practical applications. The readers can learn and master the large number of sophisticated techniques which form the backbone of the fascinating and growing field of human assisted reproduction. The subject is vast and has been covered over 83 chapters. All the chapters are dealt by the experts of concerned fields. Principles and protocols pertaining to laboratory maintenance, culture media, cryofreezing of gametes, embryos, and genital tissues have been dealt with at length. This book is an invaluable reference book for the clinicians, reproductive biologists and embryologists.

Our knowledge of reproductive medicine has expanded rapidly since the birth of Louise Brown, the first baby to be conceived by in vitro fertilization, which was performed by Professors Patrick Steptoe and Bob Edwards in Oldham, Eng land, in 1978. Hardly a year goes by without the development of a new or a modification of an existing method of assisted reproduction. Within a relatively short period, in vitro fertilization has been introduced into the treatment of female infertility. Intracytoplasmic sperm injection has also created new opportunities for the treatment of male infertility. The first edition of this book was published in 1996. In the second edition most of the chapters have been updated and additional interest is focused on intracytoplasmic sperm injection (ICSI) in view of the risk of malformations in newborns. This manual addresses the techniques of assisted reproduction that are avail able today. Competent authors from various centers present, in a concise way, their tried-and-tested procedures, so that the latter can be readily implemented. Due to different legal regulations, the scope of assisted reproduction is much more limited in Germany than in many other countries. For example, whereas only three embryos may be created and transferred in Germany, such restric tions do not exist in several other European countries and the United States. Furthermore, heterologous fertilization, oocyte donation, and surrogate mother hood are banned in Germany.

A complete guide to sperm retrieval methods performed for men with azoospermia, aimed at andrologists and male fertility specialists.

This publication will be an invaluable guide to scores of Infertility practitioners, postgraduate students and ART specialists across the world. Manual of Intrauterine Insemination (IUI), In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI) covers all the aspects of the latest technologies, including the most recent topic of Vitrification. There are numerous photographs which makes the subject appealing as well as easy to understand. Each topic has been elaborated in depth so as to impart profound knowledge to readers. Several of them have written to me about the clarity of thought and simplicity of language that have become a hallmark of Kanthi Bansal’s style of writing. Infertility management has taken a new turn in this era. With the advent of assisted reproduction techniques (ART), there is a larger area of education that is vacant. This book depicts its simple representation of the advanced techniques. The subject was covered in-depth, keeping the length of the book minimal, to facilitate quick and complete reading at one stretch. Manual of Intrauterine Insemination (IUI), In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI) aims at simplifying the clinical and laboratory aspects of advanced techniques of intrauterine insemination (IUI), in vitro fertilization and intracytoplasmic sperm injection (ICSI). The manual will be beneficial to the clinicians already practicing ART, for those who want to set-up new units as well as those who are interested in knowing about them. The book is structured in a simple manner including case studies and is expected to be more practical and interesting.

This unique, comprehensive text on the male factor infertility experience centers on the current background, applications and techniques for intracytoplasmic sperm injection (ICSI), one of the more commonly utilized procedures in modern assisted reproduction for male infertility, offering insights into the rationale and indications for ICSI and providing an up-to-date discussion on male factor diagnostic tests and microsurgical techniques for ICSI itself. Topics covered include the role of reproductive genetics, the effects of paternal age, the assessment, development and selection of functional human spermatozoa (e.g., Comet assay), both naturally and in the laboratory, and the procedures by which these sperm are injected directly into an oocyte. Comparisons are made between efficacy rates of ICSI versus conventional IVF strategies, and clinical approaches to fertilization failures following ICSI are presented, and intracytoplasmic morphologically selected sperm injection (IMSI) is discussed in detail in a chapter of its own. Bringing together the latest evidence and written by international leaders in the field, Intracytoplasmic Sperm Injection: Indications, Techniques and Applications is a unique and accessible resource for reproductive endocrinologists, andrologists, geneticists and infertility practitioners and researchers alike.

Featuring more than 300 full-color photographs, this atlas is a comprehensive guide to the complex procedures used in assisted reproduction. It shows readers how to utilize the latest technologies and correlates the laboratory and clinical components of assisted reproduction. The book depicts oocytes, embryos, and blastocysts at various stages of division and offers guidelines for assessing oocytes and embryos. The authors give detailed instructions on ovarian stimulation, intracytoplasmic sperm injection, assisted hatching, cryopreservation, extended in vitro culturing, preimplantation genetic diagnosis, and embryo transfer techniques. Close attention is also given to assessment of oocytes and embryos from patients with endometriosis and other pathologies.

Advances in technology now offer promising solutions to deal with the chronological aging of the cell, tissue or organ to synchronize its existence and its use. This book covers the developments in and benefits of the latest vitrification technology and its extensive applications in reproductive medicine. Protocols of gametes (oocyte and sperm), embryos, blastocysts and ovarian tissue cryopreservation have been reviewed by leaders in the field. In order to address the escalation in cross-border reproductive tourism entailing the transfer of reproductive cells and tissues rather than the patient, the challenges, caution and emerging possibilities of nitrogen vapor shipment of vitrified cells have been discussed. Current perspectives on oocyte banking present the reader with options and solutions to effectively utilize these gametes despite the physiological deterrents.The versatile applications and potential of vitrification of human embryonic stem cells, discussed in the concluding chapter, is an exciting reality offered by vitrification to help overcome numerous stumbling blocks in the management of various disorders.