Molecular Diagnostic Pcr Handbook

PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.

PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.

This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.

This PCR handbook establishes an easy approach for the students going through their studies in Molecular Diagnostics. In the area of molecular diagnostics, PCR has allowed target detection to be performed with unprecedented sensitivity and ease. This handbook introduces timely topics such as Clinical Applications of PCR, Real time PCR, PCR Optimization, Application of PCR, PCR Array System Performance, Designing the primers, PCR in diagnosis of diseases and different PCR Techniques and their procedures. It also briefs the techniques which can be used in diagnosis of different diseases. Moreover it contains in-depth information about the PCR Arrays including Cancer Pathway Finder PCR Array, Cardiotoxicity PCR Array, Cell Surface Markers PCR Array, DNA Damage Signaling Pathway PCR Array, DNA Repair PCR Array, Drug Metabolism PCR Array etc. It is hoped that the step-by-step protocols and the explanatory notes will help readers to harness the power of these techniques in their laboratories. In short the aim of this handbook is to provide a meaningful and comprehensive picture of PCR and their types.

The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising: - Clinical microbiology - Clinical chemistry - Clinical genetics - Clinical pathology - Molecular hematopathology - Veterinary health - Plant health - Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory.

PREFACE The Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture is involved in agricultural research and development and assists Member States of FAO and IAEA in improving strategies to ensure food security through the use of nuclear techniques and related biotechnologies, where such techniques have a valuable and often unique role. In particular, molecular diagnostic methods have rapidly evolved in the past twenty years, since the advent of the Polymerase Chain Reaction (PCR). They are used in a wide range of agricultural areas such as, improving soil and water management; producing better crop varieties; diagnosing plant and animal diseases; controlling insect pests and improving food quality and safety. The uses of nucleic acid-directed methods have increased significantly in the past five years and have made important contributions to disease control country programmes for improving national and international trade. These developments include the more routine use of PCR as a diagnostic tool in veterinary diagnostic laboratories. However, there are many problems associated with the transfer and particularly, the application of this technology. These include lack of consideration of: the establishment of quality-assured procedures, the required set-up of the laboratory and the proper training of staff. This can lead to a situation where results are not assured. This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR laboratory; Good Laboratory Practice and standardised of PCR protocols.

Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.