Monoclonal Antibody Production Techniques And Applications
Download Monoclonal Antibody Production Techniques And Applications full books in PDF, epub, and Kindle. Read online free Monoclonal Antibody Production Techniques And Applications ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Monoclonal Antibody Production Techniques and Applications
Author | : Lawrence B. Schook |
Publsiher | : New York : Dekker |
Total Pages | : 344 |
Release | : 1987 |
Genre | : Medical |
ISBN | : UOM:39015011765164 |
Download Monoclonal Antibody Production Techniques and Applications Book in PDF, Epub and Kindle
Monoclonal Antibody Production
Author | : National Research Council,Institute for Laboratory Animal Research,Committee on Methods of Producing Monoclonal Antibodies |
Publsiher | : National Academies Press |
Total Pages | : 74 |
Release | : 1999-06-06 |
Genre | : Medical |
ISBN | : 9780309064477 |
Download Monoclonal Antibody Production Book in PDF, Epub and Kindle
The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Applications And Engineering Of Monoclonal Antibodies
Author | : David J. King |
Publsiher | : CRC Press |
Total Pages | : 262 |
Release | : 1998-11-27 |
Genre | : Medical |
ISBN | : 0748404228 |
Download Applications And Engineering Of Monoclonal Antibodies Book in PDF, Epub and Kindle
A valuable resource for researchers and workers in the fields of both pharmaceuticals and biotechnology as well as undergraduates in biochemistry, applied biology, biomedical sciences and pharmacy, this book compares established techniques of antibody production with the new. Antibody structure and the implications of antibody engineering are fully discussed, and a case study approach illustrates how antibodies are finding increasing use in the diagnosis and treatment of disease. The volume ends with commercial expression, purification and large-scale manufacture of antibodies and their future potential, particularly as therapeutic agents.
Antibodies
Author | : G. Subramanian |
Publsiher | : Springer Science & Business Media |
Total Pages | : 352 |
Release | : 2013-03-07 |
Genre | : Medical |
ISBN | : 9781441988751 |
Download Antibodies Book in PDF, Epub and Kindle
If the antibody industry is to achieve its full potential in the next decade, the individual technical potentials must be exploited, the limitations must be addressed, and lessons learned must be applied both to current purification methods and to the new technologies that continue to emerge. This book presents an overview of the current advances applied in the manufacture of monoclonal antibody including: -concepts in development of manufacturing strategies, -importance of antibody fragments, -application of chromatography method development, -quality control, -effect of expression on antibody properties, -virus removal and safety, -pharmacokinetics, -regulatory aspects.
A Practical Guide to Monoclonal Antibodies
Author | : J. Eryl Liddell,A. Cryer |
Publsiher | : John Wiley & Sons |
Total Pages | : 212 |
Release | : 1991-08-26 |
Genre | : Medical |
ISBN | : 0471929050 |
Download A Practical Guide to Monoclonal Antibodies Book in PDF, Epub and Kindle
Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Monoclonal Antibody Production
Author | : Committee on Methods of Producing Monoclonal Antibodies,Institute for Laboratory Animal Research,National Research Council |
Publsiher | : National Academies Press |
Total Pages | : 57 |
Release | : 1999-05-20 |
Genre | : Science |
ISBN | : 9780309519045 |
Download Monoclonal Antibody Production Book in PDF, Epub and Kindle
The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) in early 1997 to prohibit the use of an animal in the production of mAb (monoclonal antibodies). NIH responded late in 1997, asserting that continued use of the mouse method for producing mAb was scientifically required. In a second petition, in early 1998, AAVS did not accept the NIH response. NIH asked the National Research Council to form a committee to study this issue. The Committee on Methods of Producing Monoclonal Antibodies was composed of 11 experts with extensive experience in biomedical research, laboratory animal medicine, pain research, animal welfare, and patient advocacy. The committee was asked to determine whether there is a scientific necessity for producing mAb by the mouse method and, if so, to recommend ways to minimize any pain or distress that might be associated with the method. The committee was also to determine whether there are regulatory requirements for the mouse method and to summarize the current stage of development of tissue-culture methods.
Basic Methods in Antibody Production and Characterization
Author | : Gary C. Howard,Delia R. Bethell |
Publsiher | : CRC Press |
Total Pages | : 286 |
Release | : 2000-09-21 |
Genre | : Medical |
ISBN | : 9781420036534 |
Download Basic Methods in Antibody Production and Characterization Book in PDF, Epub and Kindle
Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described
Monoclonal Antibody and Immunosensor Technology
Author | : A.M. Campbell |
Publsiher | : Elsevier |
Total Pages | : 426 |
Release | : 1991-12-12 |
Genre | : Medical |
ISBN | : 008088735X |
Download Monoclonal Antibody and Immunosensor Technology Book in PDF, Epub and Kindle
This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.