Morphology Methods

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.

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Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more pre

Atlas of Hematopathology: Morphology, Immunophenotype, Cytogenetics, and Molecular Approaches, Second Edition, will appeal to both a wide range of people undergoing training in a variety of medical fields and practicing non-hematopathologists. For clinicians, fellows and residents, correct diagnosis (and therefore correct treatment) of diseases depends on a strong understanding of the molecular basis for the disease, making this book a crucial resource. This atlas contains hundreds of high-quality color images that mirror the findings that fellows and clinicians encounter in practice. In addition, it provides information in a quick, simple and user-friendly manner, attracting both those in training and non- experts. Residents, fellows, practicing clinicians, and researchers in pathology, hematology and hematology/oncology will find this a useful resource. Covers both non-neoplastic hematopathology and neoplastic hematopathology in all organs and tissues Demonstrates results of important complementary diagnostic tests, such as immunophenotyping (immunohistochemical stains and flow cytometry), karyotyping, FISH and DNA/molecular studies Provides tables to summarize the most important clinicopathological features Saves clinicians and researchers time in quickly accessing the very latest details on the diverse clinical and scientific aspects of hematopathology

Over recent decades, the historico-geographical approach to urban morphology has been prominent in the debate on the physical form of our cities and on the agents and processes shaping that form over time. With origins in the work of the geographer M.R.G. Conzen, this approach has been systematically developed by researchers in different parts of the world since the 1960s. This book argues that J.W.R. Whitehand structured an innovative and comprehensive school of urban morphological thought grounded in the invaluable basis provided by Conzen. It identifies the development of several dimensions of the concepts of “fringe belt” and “morphological region” and the systematic exploration of the themes of “agents of change,” “comparative studies” and “research and practice” as key contributions by Whitehand to this school of thought. The book presents contributions from leading international experts in the field addressing these major issues.

This book investigates the historical evolution, regional differences, and quantitative measurement on street interface, which forms the street space and plays a very important role in urban form. Empirical research reveals the street interface in Chinese cities are much more complicated than European and American cities. This book explores the reason and reveals the relationship between street interface and urban form in morphology. By constructing quantitative measurement method on street interface morphology, quantitative parameters can be used in urban planning guidelines in China. Both researchers and students working in architecture, urban design, urban planning and urban studies can benefit from this book.